FastQCFastQC Report
Tue 19 Nov 2019
HWC7VAFXY_n01_AH5187-rep2-Top2chip-spike.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC7VAFXY_n01_AH5187-rep2-Top2chip-spike.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6035822
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATATCTCGTATG319560.5294390722589235TruSeq Adapter, Index 7 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGAGT43750.038.7788531
AGTCACG44250.038.5942828
GCACACG45600.038.0903111
GTCACGA44900.038.0859929
TCACGAG44950.037.6935430
ACGTCTG46000.037.61142315
CACACGT46750.037.24865712
ACACGTC46500.037.15861513
CACGTCT46700.037.0958314
ACGAGTG46050.036.98737332
CGAGTGG46000.036.78270333
GATCGGA46700.036.7701721
CGTCTGA47900.036.1195316
GAGCACA48300.036.1007849
GAACTCC48250.035.8625721
TCCAGTC48450.035.43382325
AGCACAC49200.035.34894610
TATCTCG46600.035.2454141
CAGTCAC49750.034.9607627
CCAGTCA49650.034.94024726
Produced by FastQC (version 0.11.5)