FastQCFastQC Report
Tue 19 Nov 2019
HWC7VAFXY_n01_AH5187-rep1-i3h-spike.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC7VAFXY_n01_AH5187-rep1-i3h-spike.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6446809
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGTAATCTCGTATG231960.35980591328205935TruSeq Adapter, Index 22 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTACG43350.040.2257733
GCACACG45300.038.6895811
CGTACGT45300.038.39217834
GATCGGA45250.038.199921
ACGTCTG46800.037.2085815
CACACGT46950.037.13762712
ACACGTC47200.037.0839213
GAGCACA48050.036.5221189
CACGTCT48100.036.43681314
GTACGTA47950.036.3170435
AGCACAC48450.036.26703310
ACCGTAC48700.035.8996832
CGTCTGA49100.035.60307716
AGAGCAC49550.035.598138
CAGTCAC49300.035.55405427
TCCAGTC49650.035.21059425
GAACTCC50100.035.03204721
AGTCACC50800.034.5928128
CCAGTCA50800.034.591226
TCGGAAG51500.034.515033
Produced by FastQC (version 0.11.5)