FastQCFastQC Report
Tue 19 Nov 2019
HWC7VAFXY_n01_AH5187-rep1-3h-Top2chip-spike.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC7VAFXY_n01_AH5187-rep1-3h-Top2chip-spike.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5235883
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG56050.040.7833111
GATCGGA56100.040.235581
ACGTCTG57650.039.37785715
CGTCTGA57750.039.3486316
CACGTCT59050.038.63476614
ACACGTC59400.038.6343813
TCCAGTC58350.038.59846525
GAACTCC58500.038.54016521
CCAGTCA58500.038.4229426
AGCACAC60050.038.36642510
CACACGT59750.038.2951112
CAGTCAC59150.038.07751527
TCGGAAG60150.038.0055773
GAGCACA60900.037.7200979
GTCTGAA60500.037.63802717
AGTCACA61050.037.1504528
CACATGT60100.037.13938531
ACTCCAG60700.037.03211223
AGAGCAC62150.036.92568
GTCACAT61700.036.5767429
Produced by FastQC (version 0.11.5)