FastQCFastQC Report
Tue 19 Nov 2019
HWC7VAFXY_n01_AH5184-i3h-spike.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC7VAFXY_n01_AH5184-i3h-spike.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5569031
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG34200.039.2080911
ACACGTC35100.038.39470713
CACACGT35500.037.89871612
ACGTCTG35850.037.52871715
GATCGGA35400.037.3843231
CACGTCT37300.036.06982414
AGCACAC37750.035.64017510
CGTCTGA38000.035.34617216
GAGCACA38350.035.1412359
CAGTCAC38300.034.66060327
GAACTCC38650.034.23275821
TCCAGTC39150.033.79282825
AGTCACC39600.033.57987228
CCAGTCA39600.033.57957526
AGAGCAC40100.033.4393238
TCGGAAG41900.032.059073
CCAGATC40950.031.9774733
GTCTGAA41850.031.93751717
ACTCCAG41700.031.62123
ATCGGAA44500.030.3898472
Produced by FastQC (version 0.11.5)