FastQCFastQC Report
Tue 19 Nov 2019
HWC7VAFXY_n01_AH5184-3h-Top2chip-spike.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC7VAFXY_n01_AH5184-3h-Top2chip-spike.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5558562
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATG148850.26778508542317236TruSeq Adapter, Index 14 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG38450.038.4440511
CGTCTGA39250.037.25855616
ACGTCTG39450.037.01263415
GATCGGA39900.036.8320121
CACGTCT39950.036.7183514
ACACGTC40100.036.58099713
GAACTCC39900.036.14800321
CACACGT40750.036.10791812
CCGTATC38850.035.5010638
AGTTCCG40300.035.34032434
AGCACAC42150.035.1761210
TCCAGTC41050.035.02255225
GAGCACA42300.034.8918159
CCAGTCA41950.034.5399626
GTTCCGT41400.034.34667235
CAGTCAC42250.034.18973527
TCGGAAG43750.033.9426353
AGAGCAC43850.033.7097788
GTCTGAA43750.033.32699217
GTCACAG43800.032.92875329
Produced by FastQC (version 0.11.5)