FastQCFastQC Report
Tue 19 Nov 2019
HWC7VAFXY_n01_AH4890-input.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC7VAFXY_n01_AH4890-input.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6961283
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG7450.010.87179111
ACACGTC8900.09.60599213
CACACGT9400.09.33437712
CGTCTGA10100.08.24193116
CACGTCT11250.07.79939114
ACGTCTG10400.07.787853215
CAGTCAC12200.07.192888327
GAACTCC12650.06.937412721
TCCAGTC13000.06.750200325
CACTAGC13000.06.57716531
GAGCACA13750.06.3814129
GATCGGA12250.06.24620531
CCAGTCA14000.06.10736826
AGGGGGG6657.159435E-66.09150031
TCACTAG15550.05.932694430
ACTAGCT15250.05.90151832
AGAGCAC15100.05.8108888
ATCGGGG4700.0055102135.7444840
AGCACAC14100.05.74431510
GTCACTA17900.05.5309329
Produced by FastQC (version 0.11.5)