FastQCFastQC Report
Tue 19 Nov 2019
HWC7VAFXY_n01_AH4890-input-resonic.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC7VAFXY_n01_AH4890-input-resonic.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6169022
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCACGG7000.017.036429
CACGGCT7250.016.13858231
GCACACG7400.015.506000511
AGTCACG7850.015.47833128
CGGCTAC8100.014.16723833
TCACGGC8300.014.09695330
ACGTCTG8100.013.88787315
ACGGCTA9000.013.25053532
GGCTACA10000.012.37489734
CACACGT9550.012.01482912
CACGTCT10050.011.86480414
AGCACAC10150.011.74819510
ACACGTC10600.011.46142613
CATCTCG9900.011.13627139
GTATGCC10400.010.60164645
GATCGGA10200.010.5908961
CGTCTGA11050.010.587460516
CCAGTCA12350.010.38436126
CAGTCAC11500.010.36981627
GAACTCC12250.010.28596221