FastQCFastQC Report
Tue 19 Nov 2019
HWC7VAFXY_n01_AH4890-Myc.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC7VAFXY_n01_AH4890-Myc.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7181114
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTATG115400.1606993009719662TruSeq Adapter, Index 20 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCACG23150.035.76826528
GCACACG23650.035.579211
ACGTGGC24700.033.70565832
GATCGGA24950.033.6433941
CGTGGCC24650.033.59146533
CACGTGG24750.033.54712331
ACGTCTG25650.033.1558515
CGTCTGA26450.031.8978416
CACACGT26400.031.7878112
ACACGTC26750.031.70832313
GTCACGT26350.031.59527629
CACGTCT27250.030.96139314
TCACGTG27200.030.69086330
AGCACAC27800.030.42977510
GGCCTTA27600.029.67228736
CAGTCAC28350.029.60423327
GTGGCCT28050.029.27728534
TCCAGTC29350.028.44105525
GAGCACA30300.028.4388399
CCAGTCA30350.027.8004926
Produced by FastQC (version 0.11.5)