FastQCFastQC Report
Tue 19 Nov 2019
HWC7VAFXY_n01_AH4890-Myc-resonic.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC7VAFXY_n01_AH4890-Myc-resonic.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7247358
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG13700.030.21692511
AGTCACG15200.027.68209628
GATCGGA15600.026.6908251
GTCACGT16400.025.38217529
CACACGT16800.025.04294212
CGTTTCG16400.024.96919433
ACACGTC17050.024.67574113
CGTCTGA17000.024.48363316
CACGTCT17500.024.2983514
ACGTCTG17650.023.83690815
AGCACAC18950.021.96420710
CAGTCAC20450.020.68533127
GAACTCC20550.020.4761721
GTTTCGG20400.020.2931634
TCCAGTC21400.019.97734625
ATCGGGG9850.019.87188542
TCGGGGG5500.019.63597743
GAGCACA21250.019.5869069
AGAGCAC21900.019.005828
CCAGTCA22650.018.87523526