FastQCFastQC Report
Thu 14 Dec 2017
HWC5LBGX3_n01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5LBGX3_n01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences305662541
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGCTGTGTCGGTCTCGTAGTACGGACTTCCAGTTATGCTATCGATGAATTCGAGCTCGTATCTCGTATGCCGTC19281320.6308041520861399No Hit
TATGGGTGTCGGTCTCGTAGTACGGACTTCCAGTTATGCTATCGATGAATTCGAGCTCGTATCTCGTATGCCGTC17465920.571411856449888No Hit
GCAGCGTGTCGGTCTCGTAGAACCACTGCGGACATAGGAGATCGATGAATTCGAGCTCGTATCTCGTATGCCGTC11578020.3787843928183532No Hit
CCGCTGTCCACGAGGTCTCTTTATAGAGGCTCCCACTCGGCGTACGCTGCAGGTCGACATCTCGTATGCCGTCTT9259850.30294356546620477Illumina PCR Primer Index 11 (95% over 21bp)
AGGTTGTGTCGGTCTCGTAGTACGGACTTCCAGTTATGCTATCGATGAATTCGAGCTCGTATCTCGTATGCCGTC9230540.30198466484645237No Hit
ATACCGTCCACGAGGTCTCTTTATAGAGGCTCCCACTCGGCGTACGCTGCAGGTCGACATCTCGTATGCCGTCTT7215880.23607341535513832Illumina PCR Primer Index 11 (95% over 21bp)
GCGGTGTGTCGGTCTCGTAGTACGGACTTCCAGTTATGCTATCGATGAATTCGAGCTCGTATCTCGTATGCCGTC7052590.23073124946638457No Hit
TATGGGTCCACGAGGTCTCTTTATAGAGGCTCCCACTCGGCGTACGCTGCAGGTCGACATCTCGTATGCCGTCTT6980650.2283776735337681Illumina PCR Primer Index 11 (95% over 21bp)
CAGACGTGTCGGTCTCGTAGTACGGACTTCCAGTTATGCTATCGATGAATTCGAGCTCGTATCTCGTATGCCGTC6673510.21832933725431536No Hit
AGCACGTGTCGGTCTCGTAGAACCACTGCGGACATAGGAGATCGATGAATTCGAGCTCGTATCTCGTATGCCGTC6048630.1978858770267175No Hit
GCGGTGTCCACGAGGTCTCTTTATAGAGGCTCCCACTCGGCGTACGCTGCAGGTCGACATCTCGTATGCCGTCTT5993870.19609435884392518Illumina PCR Primer Index 11 (95% over 21bp)
TGCGGGTGTCGGTCTCGTAGTACGGACTTCCAGTTATGCTATCGATGAATTCGAGCTCGTATCTCGTATGCCGTC4663260.15256236451950456No Hit
CTCAGGTGTCGGTCTCGTAGAACCACTGCGGACATAGGAGATCGATGAATTCGAGCTCGTATCTCGTATGCCGTC4648300.15207293588519896No Hit
CAGACGTCCACGAGGTCTCTTTATAGAGGCTCCCACTCGGCGTACGCTGCAGGTCGACATCTCGTATGCCGTCTT4250150.13904713302766136Illumina PCR Primer Index 11 (95% over 21bp)
GCAGCGTCCACGAGGTCTCTACGACCGTCTAACTATGCAGCGTACGCTGCAGGTCGACATCTCGTATGCCGTCTT3755180.1228537846906141Illumina PCR Primer Index 11 (95% over 21bp)
TGCGGGTCCACGAGGTCTCTTTATAGAGGCTCCCACTCGGCGTACGCTGCAGGTCGACATCTCGTATGCCGTCTT3680110.12039780824827993Illumina PCR Primer Index 11 (95% over 21bp)
GCTCGGTGTCGGTCTCGTAGTACGGACTTCCAGTTATGCTATCGATGAATTCGAGCTCGTATCTCGTATGCCGTC3479510.11383501519736433No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTCGG116123200.068.82466
TGTCGGT116184500.068.797247
TCGGTCT116369750.068.774589
CGGTCTC116348900.068.7589410
GTCGGTC116316050.068.757148
CTCGTAG113648900.068.66209414
ACGAGGT104752300.068.6564410
GGTCTCG116488150.068.61777511
GGTCTCT103815950.068.5879714
CGAGGTC105401900.068.5702611
TCGTAGA34229900.068.5685615
AGGTCTC105120900.068.5381413
TCGTAGC23141150.068.5199915
CCACGAG106160950.068.515598
CACGAGG105049150.068.515269
GTCTCGT116053600.068.51269512
GAGGTCT105355050.068.4788712
CCTGTGT4486950.068.46163
CGTAGGG4740150.068.44337516
CGCCGGT2346800.068.437391
Produced by FastQC (version 0.11.2)