FastQCFastQC Report
Thu 11 Jul 2019
HWC5JBCX2_l02_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l02_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14462406
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN3474732.402594699664772No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGCCC3950.064.653114145
TAGTAAC18300.052.1622282
GCGTCGG4050.046.842148145
AGCGGCC7950.042.21912145
CGCGTCC3400.040.774925145
CACGCAC7200.040.200293
CGCACTT8000.039.0259785
ACGGCCC5550.038.1262145
TAACAAG26200.036.3032345
TCGGCGG3450.035.954094145
ACGCACT8700.035.7853664
GCGCTCC4700.035.7066145
CGTCGCC3300.035.37729145
GCCGCCC6850.035.15134145
AGGCGCC5700.034.56268145
GGCGCCC13100.034.533367145
GTACCCC2551.8189894E-1234.33678145
TTAGGGG3650.033.984005145
TCAAGCC10200.032.906082145
CACGAGC9150.032.640198
Produced by FastQC (version 0.11.5)