FastQCFastQC Report
Tue 2 Jul 2019
HWC5JBCX2_l02_n02_SM2_DEMUX_P2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l02_n02_SM2_DEMUX_P2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences148
Sequences flagged as poor quality0
Sequence length151
%GC48

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCACGTGGTCGTCGGTGAGGTCGCCGGTGGCGATGACCTTGATGCCGGCC21.3513513513513513No Hit
AAGGTTTCCTGAGCAACGCTAATCGGATCAGGGTTAGTCGGGGCCTAAGG10.6756756756756757No Hit
GCAGTACCCTGAGTACCGCGGGGTCGGAGACGCCCTGTGGGAATCTGTCA10.6756756756756757No Hit
AGGGAAAGGTGAAAAGAACCTCGAACAGAGGAGTGAAAAGAACCTGAAAC10.6756756756756757No Hit
GACTTGCCCCAAGGGATCTCAATACTCCAATGTTGCTTTGCAGTTCTTCT10.6756756756756757No Hit
TTGGAATATGGGACGGAGAAGAGGAGTTCCATGTGAGATGTGGTGAATGC10.6756756756756757No Hit
CTGCTGAGGGTCGAGAAGAAATACAGCGCCAACGACACCGCCCGCGTGCT10.6756756756756757No Hit
CCTCCACTCCGCTCCGTGACTCTCCCATTCGCCATGTTTCCGATTTGTTT10.6756756756756757No Hit
GGTCAATGTCACTGTCGTGATACCACGGACAACAACACCCACAAAAACTT10.6756756756756757No Hit
ATACGTGGCTGAACCACTTAACCTTGCCAGACAAGAGTAACTCGTAGGCT10.6756756756756757No Hit
AAGCGGAGCGATACACGGTGATAGCCCTGTACACGTATAGAATGCTAGCC10.6756756756756757No Hit
GAGCTGAATTGATCTCTTATCTCCACATAATTTATGACAGCTCCTGGGAC10.6756756756756757No Hit
CTTTGATTATCATTGAAGGGAGTGGTTCGAATGATGAAATTTCCGGTTCA10.6756756756756757No Hit
GTTTATGTGGTTGATTTATAGTGTCAATGGTCTGTGTACTTGAGTGTGTT10.6756756756756757No Hit
GACTCCGGTTTGCCAAAGGCGGTACGTGCGCTGTGGCTGAAGTGAGCCCG10.6756756756756757No Hit
TTTCTAGCCATTATTTCTTCAAAATTTTTTCTACTTTGCTTTTTCTCTAC10.6756756756756757No Hit
TCCTTTTACTTTCAGGCTCACTTTTGTTGTGGGTCTTTATTCTTGACTCT10.6756756756756757No Hit
GAACCTACTTCTCCAGCCTCAAGTCATGTACTTTATGCTCTAATAATACC10.6756756756756757No Hit
ACGACACCAAATTAGCGCGAAATGTTGAAGAGAAGGGAATCGTGGATTGA10.6756756756756757No Hit
AATGCAATTAGAGAGACTCATACTACTGATACGTCAATCGTGTGCGACAC10.6756756756756757No Hit
TTTTAATTCATTTTGATTCGATTCAATTCTTTTCGATTTCATTCGATTCG10.6756756756756757No Hit
CGACTGTTGCCACTACCACCCATTCTATCCCGGACAGTACTTCTACATCC10.6756756756756757No Hit
AGGACAGAGAACTGGTCAGAGGCTGAGATGGACAAATTGACAGAAGTAAG10.6756756756756757No Hit
AGTGAACAGGCAGCAGAGGCCATGGAGGTTGCTAATAAGACTAGGCAGAT10.6756756756756757No Hit
ATCCAGAACAGCATAACAAAAGAAAGGACGATGCTTCTGACTTTGAATGA10.6756756756756757No Hit
GCCTTCCAGCGCGAAGGCGAAGAACATGGTGTTCTCGCGGCCATAGCGAT10.6756756756756757No Hit
CACTGTCTATGTGCGAGTCCTCAAGCCCCCAGAAATCCTAAAGGTCTTCG10.6756756756756757No Hit
CTTTACTTCCCTCCTTTTCAAAACCCAACCATTCATTGAACCAGTATGCA10.6756756756756757No Hit
GATCTATACTAACAAAAACAGAAAAGAAAAAGAACACAGATGTATTCTCC10.6756756756756757No Hit
TATGTACCCAATCTCACCAAGGTGAGCCATTGCTTTCTCAAAATCATCTT10.6756756756756757No Hit
GTAGTAACAAGAGCACCTTAGGATTCTCTCCTCGACTACCTGTGTCGGTT10.6756756756756757No Hit
TGTCGTATCCTTGTATCGAGGATTCTTGTTCAACAATTGTTTCCATTTGT10.6756756756756757No Hit
CTGCTACTCCATGTGCCCCATGGGATGTGTATCCGTGCCAACCTGCAATC10.6756756756756757No Hit
GTGTGAATGTGATGCTTGTTTCTCGCATAAAGCACAGAGCGTTCCTAGTT10.6756756756756757No Hit
TCAACGATAACATGTGGAGCACTTTTTCCAGAATGAAAATTTGAGCCTCC10.6756756756756757No Hit
GAATGGGGCGGTCCGGCGGCGTACCAGTTCGGCAACGTCGCCTGCGGTCA10.6756756756756757No Hit
CGCCACGAAGGCGGCCAGCACGCGCTGCAGCTCGGGAAAGCGCTCCCAGC10.6756756756756757No Hit
GCCCAAGGGTAGATCACAAGGTTTCGCGTCTACCTCATCTGACTATGCGC10.6756756756756757No Hit
GTTCTCTAACGATTAGATACAAGAATGACACAGACACCCCCCTGTGGTGA10.6756756756756757No Hit
GGTAGGCACAGTAGCTGAGAGTCCGGTAGGTGGTGCGCTCGCTGCCGTGA10.6756756756756757No Hit
GTCTTAATAGGGCGCATAGTCAGATGAGGTAGACGCGAAACCTTGTGATC10.6756756756756757No Hit
CCATGGTTCCACGGTGGTTTCACTAAGATATTCCACTGGTACAGTGGCTA10.6756756756756757No Hit
AGGACCGACAACCTAATATCACACAGGGCCGTGCCCTTCTTAAACAACGC10.6756756756756757No Hit
CACATGGGGACTGGTGGGGAGTGAAAGGATACAGGAGAAATGAGAGGACC10.6756756756756757No Hit
GGTGAGGACCAGCCCATCGTTCAGCTTCGGTGGGGTCACGTACAAAAGGA10.6756756756756757No Hit
GTATATACTGCTTGTTCCACCAGCAACTGGGAGAAATCTTGTTTTCCGCA10.6756756756756757No Hit
GTCAGAGAGTACTTCCTTCATTGTTTCTTTGGCCTGGATTTCTTTTTGGT10.6756756756756757No Hit
GCCTAGGCATAAGCATAAACCAGTCTCGTGACATTTCCTCGAGGGTCATG10.6756756756756757No Hit
GTAAATATTAGCAGTGCCATTACACAATCGATCCTGCTGGGTACACATAT10.6756756756756757No Hit
GCCGACCGATGTGTCGGAGGCAGCCCGCATTGACGGTGCTAGCGCATGGC10.6756756756756757No Hit
GCAGACCAGACGCCTTGGTCCACGTCACTACCCGCGCGAAGCACCCGGAT10.6756756756756757No Hit
GAAGAGCCCGAGGACTTCAACCGCGCGGTCACCGACTTCCTGGCGCGCAC10.6756756756756757No Hit
GTAGAAACACGTGCTATTTCCCAGTTTGATTAGCCTTTCACCCCTACCCT10.6756756756756757No Hit
TCTTCATCCTCCACTGTAAGATCATCAGTAGCAACAAGTTTAGCAACAAG10.6756756756756757No Hit
ATTGAGATCCCTTGGGGCAAGTCAGAAAAATGGGGAAGGAATTGCAAAGG10.6756756756756757No Hit
TTTATAACCTGAAGCTTAGCGGGTTTTCTTGGAAGTCTGTTTACCTGCTC10.6756756756756757No Hit
CCCGCGCGCGATAGGCCGCAATCATGAACATCTTCCGGATACTGGGTATG10.6756756756756757No Hit
GTTACTGTGAGAGTTAAATGAAATAATATGTGCAAAGCATGTCTTACTAC10.6756756756756757No Hit
ATGAAGCACTCCCAAGGCATGCAGAACCTTTATCTTGCCTGAAGGGCCCG10.6756756756756757No Hit
TATCCCTTCAAACTTCTGACCTAGCTGTTCTCGCCATTTCCCGTTTCTGT10.6756756756756757No Hit
GGATTTGCCTACCGTCCCTATACCTACGCCTTTTAACGAACTATTCCGTC10.6756756756756757No Hit
CCACCTTATTTCCTCAAATTTCCGTCCCAATTGTTCCCGCCACTTCTCAT10.6756756756756757No Hit
GATCCTGATCGTCCCGATTTTCGGCACTGCGATCGTCTCGCTGCTGTACG10.6756756756756757No Hit
ACCCTTGGGCAGGTTGAAGTTGCAGTAACATGTAATGGAGGACCGAACCG10.6756756756756757No Hit
GGAGGCAGCAGTTGAGTAGTAAAGCAGTATAGTATGATTATCCAATCCAT10.6756756756756757No Hit
GCTCATCGCTCCAATAAAAGGCACTAGTTGATCAAGTGATCGGAAGCAGA10.6756756756756757No Hit
GATCTGCTCGCCCCAGCGTTCCTTGACATAGGAGCGCAGGCGCAGCTCTT10.6756756756756757No Hit
GGTTAAACGAGGACGTAAACACCCAGAAACAAGGGAAACTCCGCTAGTCA10.6756756756756757No Hit
CTTCCACTGTTTGTGGGTTCGTTGCATTTTTGTAGTTGTCCTTTGGAACA10.6756756756756757No Hit
CTACAGCAGAGGGGACCAGCATTTTCTTCAGCTTTCTCTCAAGCGCCAAG10.6756756756756757No Hit
CCAAAACTGTTTCACCCATTTTTATTATCTCTTCGGCAGAAAGCCCCTCA10.6756756756756757No Hit
CTAGGAACGCTCTGTGCTTTGTGCGAAAAACAAGCATCACATTCACACAG10.6756756756756757No Hit
CCACATAAGTATGTACTGGCAGAGAAGGTAATGGTGAAATTTCTCCAACT10.6756756756756757No Hit
CTTACCTACCGTCGTCTAACAGCCATTCACAGATCTCTGATTTGGCCAGC10.6756756756756757No Hit
TTGGTGATGTCGTTTCGACGTTTGACGCTGGAGGCGCCGCATTGTTTGCA10.6756756756756757No Hit
CTCGTAGGCTCATTATGCAAAAGGCACGCCGTCACTGGACCTGCCAGCTC10.6756756756756757No Hit
CTGTAGGGTCCTTGTGTAGCAATTTGACTTCGACTTTTCCTTGTCATTAA10.6756756756756757No Hit
AGCAGGTGCCCGATGTGCGCGCTCGGGGCGGTCGGGTCGATCCCGGAGTA10.6756756756756757No Hit
ATAATGAGATCAGATGCACCCATTGGCAAATGCAAGTCTGAATGCATCAC10.6756756756756757No Hit
TGTGACGAGACTGGTTTCTGCTTATGCCTAGGCAAAAGATAATCGGCCCT10.6756756756756757No Hit
GTGGTGACGGAGTAGTGACACTGCCGCGGACTGACGGAATAGTTCGTTAA10.6756756756756757No Hit
CCCATACATTAAAGAATTTAAGTTGTTCTGTAAGGTGATAAATATTTTCA10.6756756756756757No Hit
GCAGATAGCCGGGCTCCACGGGCCGTTCCCGTGGAGCCCGGCTATCTGCC10.6756756756756757No Hit
CTCAAGTACCCCTCGGTGGAGTCGGTAACGCTGGTGGAACTGGACCCGGC10.6756756756756757No Hit
TCCGACATCTCATTCCACGAGTTGATTGTGAGTCGCTCGATCTTTGCGAG10.6756756756756757No Hit
GTTATTCCCGTTACCCCTTCCGGAGGCTAGACCCACATGGCGCTCACCCT10.6756756756756757No Hit
GTTTGTTCAAGTGGAAAATCAAAGGACATCAGACTATCACGATTCAAATA10.6756756756756757No Hit
AACAATCACACACTCGACACGAATTCGACCAGACAAACCGTAGACCCTGA10.6756756756756757No Hit
GTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTC10.6756756756756757No Hit
GATTACCAACGTAACTCTCTGGTTATTCCTCACATTCATGTACCTTTCCT10.6756756756756757No Hit
ACTCCTGAGAGACCGATAGTGAACTAGTACCGTGAGGGAAAGGTGAAAAG10.6756756756756757No Hit
AGGCAATACCATTCAGGACATAGGCGTGGGCAAGGACTTCATGTCTAAAA10.6756756756756757No Hit
GGGAATCTAATTGCTCCTAGGGTTTACTTCAAAATACGAAATGGGAAAAG10.6756756756756757No Hit
GAGAGGTATCAATTTTGAGACACAGTTACCAAAAAGAAATTCAGGCTAAG10.6756756756756757No Hit
GAAGATCGACGCGGCCACGGGCCTGGAGATCTCCGTGGGCAAGCAGCCCG10.6756756756756757No Hit
GCCAAGACCAACCAACAGTGGCACTGTTTACACAACAAAAGGATATGCTG10.6756756756756757No Hit
TAGGTTGGAGTGTGGCAATAAGGATAAAAGAAAGGGGTTGAGGTGAGTGG10.6756756756756757No Hit
GGGCAGGGATAACAAAATCTCCAAGAAGAGGGGTGAGCCATGCTATTGGA10.6756756756756757No Hit
ATCTCTGCTCACTGCAACCTCTACCTCCCGGGTTCAAGTGATTCTCCCGC10.6756756756756757No Hit
ATGCAGTGCACGCTCAACGGGCATGGCCAGCGTGACCACAAAGCCTAAAA10.6756756756756757No Hit
CCGCGGTGGCTACAGACATGTGCTCATTCTGTCACCCAGGTGAGACACTC10.6756756756756757No Hit
ATATAGGGCGATGCATAGAGCAGGCCGGCCGCAGCCACGCAGAGGGCGGC10.6756756756756757No Hit
GCGGGAACAATTGGGGCGGAAAATGGAGGAAATAAGGTGGTTAATTGAAG10.6756756756756757No Hit
AACACGAGCTATTTCCCAGTTTGATTAGCCTTTCACCCCTACCCTCCGGT10.6756756756756757No Hit
AGCATCTCTAGCATTGGAAGGACAAGGGCAAGAACAATCCTTGAGACCAC10.6756756756756757No Hit
CCCTTGGTCTTTCGGAGGACGTGTTTCTCACACGTCTTTCGCTACTCATG10.6756756756756757No Hit
AGCGGAGCATTTTCTAATATCCACAAAATGAAGGCAATAATTGTACTACT10.6756756756756757No Hit
GAACTGGAGTGCCTCGCGCGTCACGTCCGTCTCTGCCAGCATCAAGACGG10.6756756756756757No Hit
GTTTCCTAACAGAATCATTAGCAAGATATTGTCTGCCCCTCGGTTCTATC10.6756756756756757No Hit
GGTCAAGACCGTCTAAACAGACTAAAGAGGAAATTAGAGTCAAGAATAAA10.6756756756756757No Hit
CATGTTGCACACACCAGGCCAAATGCCACTTCAGTGGTTACAGCCCCCAT10.6756756756756757No Hit
TCCAAGGGCTTGGGGCAGGATCCCTGCTGGTCCAGGCTGGATCCCCCCAT10.6756756756756757No Hit
ATACTGACTTAGCCAATAGAGTCCTACAGCCTTTCCCCGTGGCACCTTCT10.6756756756756757No Hit
CCGCATGCCCCGCGGAACCAAGCCATCGGTGGCACTTTCCTGACAACAGG10.6756756756756757No Hit
ATTAAGACTCGCTTTCGCTTCGGATACGTGGCTGAACCACTTAACCTTGC10.6756756756756757No Hit
GCCTTCAGGATTGCGGCTTGCGCCTGACCTTCAGCGCGCAGAATCGCAGC10.6756756756756757No Hit
CGATGGGGTTAGGCGGCATGGAGGATCGTCGGATTGGTCAGCTCTCCGGG10.6756756756756757No Hit
TCTTTTCAGTCTTCAGAATTATCAATTACTATGAAAATTTACTTGCATTT10.6756756756756757No Hit
CTCAGGATGTTTCATTCCTCTCGGCTTTTGTGTGTGTGTGTGTGTGTGTG10.6756756756756757No Hit
CTCCATCAGAAGGTAGATGCCTATCTTTTTGTGGAGAAAACAAGATACAG10.6756756756756757No Hit
CTCAAACTGTGTGGCCCTGGGCAAATTCTTTAATCTTTTTTGGGTTTCAA10.6756756756756757No Hit
GCCATATTGCCCAGGCTGGTCTTGATCTCCTAGGCTCAAGCAATCCTAGG10.6756756756756757No Hit
GCATGAACCAGTCTCGTGACATTTCCTCGAGGGTCATGTCACGAGACTGG10.6756756756756757No Hit
GTCTTATAGAGGTAGAGCTACCGATTGGGTGCGGGGGAGTCAAATCCTAC10.6756756756756757No Hit
CCTCGGTTTACTCCAGCTACCTTATGTGTTGGCCATCACTGGCCTGCTCT10.6756756756756757No Hit
CCTCCCGTACTACTAGTACGAACCATCAGTACGCCTACAACTCACTTGCC10.6756756756756757No Hit
TTTTTACTCCAATTGCTTTTCTCTCTTCAAGAGACATCCTTTTACTTTCA10.6756756756756757No Hit
CCATTATCACATTGCTCGCCTCATCAGGAGGCATTTCCTTGGTGAGAGGG10.6756756756756757No Hit
GGTTAAGTGGTTCAGCCACGTATCCGAAGCGAAAGCGAGACTTAATAGGG10.6756756756756757No Hit
GTTCTTATCCATGACCGCCTGGTCCAATCGCACGCAAAGAGGGCCTATTA10.6756756756756757No Hit
GCAACGCACCGGCCATGACCGACGTGATCGGCGGCCAGCTGAGCATGATG10.6756756756756757No Hit
GTTGTGAGAGACTTTGAAGTACACTATACAAACAAATAAACAAATAAGTT10.6756756756756757No Hit
ATACTGAAATATGTATGTCTGGTAGGTGAGCATTCTATTTACGGCCAAGC10.6756756756756757No Hit
TGCACAGCTCATAACGGGCGACGTTGTTCTCTTATAAACCTTAACGTACA10.6756756756756757No Hit
GTAGAAACACGTGCTATTTCCCAGTTTGATTAGCCTTTCACCACTACCCT10.6756756756756757No Hit
CAACGCACTTTTTCCAGTTTATTTGCTGACATTGATTACAATTTGCTTAA10.6756756756756757No Hit
TACTTGTAGTTGGGCCATTCCTATTCCACCATGTTACGGCCAGATGTGAT10.6756756756756757No Hit
CATCTGACTATGCGCCCTATTAAGACTCGCTTTCGCTTCGGATACGTGGC10.6756756756756757No Hit
ATAGTGTGCAATAAAAAAAGAAGTAAACTCCGTGATCAATAACATGAAAG10.6756756756756757No Hit
GTCTGAACTAAGACACGGCGCTTGGAAGTCTGCCCTCAACGAGCGCGACA10.6756756756756757No Hit
GTCCTCCGCTCGCCAGCACGCTCAGGTACGGAAAGGCGGGTTTCAGCTTC10.6756756756756757No Hit
ATGTTGCCCTACTGGTTTCCTGTATGATGCACTGTTAAAACAGGCCTCAA10.6756756756756757No Hit
GCACAGCGATACACGGTGATAGCCCTGTACACGTATAGAATGCTAGCCTA10.6756756756756757No Hit
AGGCCCGCGGCGAGCACATCAGCGACATGCTCGTGAACCAGCGCGAGGAC10.6756756756756757No Hit
AAACAGAATCCAACTGAGGAGCAAGCCGCAGACATATGCAAGGCCGCAAA10.6756756756756757No Hit
GTTCTCTAACGATTAGATACAAGAATGACACAGACACCCCCCGGTGGTGA10.6756756756756757No Hit
GTGATAGCCCTGTACACGTATAGAATGCTAGCCTAGCAGTACCCTGAGTA10.6756756756756757No Hit

[WARN]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers