FastQCFastQC Report
Tue 2 Jul 2019
HWC5JBCX2_l02_n02_SM2_9974_P2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l02_n02_SM2_9974_P2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1891839
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG90610.47895196155698244No Hit
GATATACAGAAAGCACTAATTGGTGCCTCTATCTGCTTTTTAAAACCCAA75790.40061548577865236No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT73130.3865550926902342No Hit
ATATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTCAT62620.33100068240479236No Hit
GTGTTAAGTCATCCATGCTTTGACATTTTTCAGGAAACCTTCTATCTTAG61560.3253976686176783No Hit
GAGTGTTCCTAGTTTTACTTGCATTGAATAATTTCCAGGATTCAGGTACA59080.3122887307006569No Hit
CTTTTCATACAGTATGGCTCAAACCCTTCAATTCCAGCTGATGGATTCAT57300.30287989622795597No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA56830.3003955410581979No Hit
CATTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAGCTCTGCTT53370.2821064583191276No Hit
CTACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAAT53020.28025640659696727No Hit
GGTCAAGACCGTCTAAACAGACTAAAGAGGAAATTAGAGTCAAGAATAAA49900.26376451695942416No Hit
GTGTGAATGTGATGCTTGTTTTTCGCACAAAGCACAGAGTGTTCCTAGTT48910.2585315135167422No Hit
GTATGAAAAGTTCCTCAGAGAGCAACTGTCCGAAATACAGTTGGATTGAT47900.2531927928327939No Hit
GCTTTAGCACTTCCATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGA46330.2448939893933892No Hit
GCCTTGGAATGGATAAAAAACAAAAGATGCTTAACTGATATACAGAAAGC43600.23046358596053892No Hit
CAATATGACCACAACACAAATTGAGGTGGGTCCGGGAGCAACCAATGCCA42940.22697491699875094No Hit
ATCAAAGGCTGTGCCCTATCCTGGCTTAGGTCTTACCTCTCTGACCGACT42120.22264051010683256No Hit
ACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGC41330.2184646790768136No Hit
GTTCATAGCTGAGACCATCTGCATTTCCCGTCTCACTCCTGGCACTGAGG41140.21746036528478377No Hit
TGACTACCCTGGTCAAGACCGTCTAAACAGACTAAAGAGGAAATTAGAGT39180.2071000756406861No Hit
GTATGGCTCAAACCCTTCAATTCCAGCTGATGGATTCATAAATAGAAGTA39080.2065714894343546No Hit
ATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAG39060.20646577219308831No Hit
GTAGTAACAAGAGCATCATCACTTCTGTTGGATGTTTGTGAGATGTCATC38510.203558548058265No Hit
GAGCCATACTGTATGAAAAGTTCCTCAGAGAGCAACTGTCCGAAATACAG36200.19134820669200708No Hit
CTCTTGAAGAGAGAAAAGCAATTGGAGTAAAAATGATGAAAGTACTTCTA35640.1883881239365506No Hit
GTCCTATTGTCCAAGCCATGAAATGGAGAGCTGATAAAATAAAAGAACAA35440.1873309515238876No Hit
GGTCATATGCATTCAATCTATGCAGAGTTGATAAGGACTTGTATCCATTG35350.18685522393818924No Hit
GCTATGAGCTCTGTGTGAATGTGATGCTTGTTTTTCGCACAAAGCACAGA34500.1823622411843714No Hit
AGTAGAAACAACGCACTTTGGTTGTTGGTATCAAAGGCTGTGCCCTATCC34040.17993074463524644No Hit
CATTCACACAGAGCTCATAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGT33760.17845070325751822No Hit
ATCCTGGCTTAGGTCTTACCTCTCTGACCGACTTCAATTTGTACATGTCC33470.17691780325915682No Hit
CTTCTGACTTGCCCCAAGGGATCTCAATACTCCAATGTTGCTTTGCAGTT32640.1725305377466053No Hit
ACATTACGCATATCCCTTCTTATTGTCAAACGGAACTTCCCTTCTTTCTG31730.16772040326898854No Hit
CTGTAAGATCATCAGTAGCAACAAGTTTAGCAACAAGCCTTCCACTCTGG29370.15524576879956486No Hit
ATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGACTTGCCCCAAGGGA29230.15450574811070075No Hit
GTAAGAAATACAGTAAAAATTGAATTTAATGTGATTCTTCTACCTCCAAA29210.15440003086943443No Hit
AGCAGGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT29120.1539243032837361No Hit
GTATTGTCCCTGAGAGTATTAGTAAACGGAACATTCCTCAAACACCCCAA29100.15381858604246978No Hit
GCACTAATTGGTGCCTCTATCTGCTTTTTAAAACCCAAAGACCAGGAAAG28260.14937846190928508No Hit
CTATAGAACATGCTTGTAAATTCAAACATTCCAGTTTCGTTACAGTAACT28170.14890273432358672No Hit
CTGATATACAGAAAGCACTAATTGGTGCCTCTATCTGCTTTTTAAAACCC26840.14187253777937764No Hit
AAATTACACTGTTGGTTCGGTGGGAAAGAATTTGACCTAGACTCTGCCTT26580.1404982136429157No Hit
CTCAGGGACAATACATTACGCATATCCCTTCTTATTGTCAAACGGAACTT26490.14002248605721734No Hit
TCTGTGACCAGTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTT26100.13796099985252444No Hit
GTCATGTACCTGAATCCTGGAAATTATTCAATGCAAGTAAAACTAGGAAC26040.13764384812872554No Hit
GCACAGAGTGTTCCTAGTTTTACTTGCATTGAATAATTTCCAGGATTCAG25420.13436661364947017No Hit
GCTCATAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGTGAGACGGGAAAT25360.13404946192567124No Hit
ATCTTAGGTAGTTTAGGTTGGTATAATTTCATCAGGTTTCATGGATAGGT24910.13167082399717947No Hit
GCTATGAACACAGCAAAAACAATGAATGGAATGGGGAAAGGAGAAGACGT24860.13140653089401372No Hit
GTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAGTAAAA24820.1311950964114811No Hit
CTCCAATGTTGCTTTGCAGTTCTTCTGCCAGTTTTTGGACGTCTTCTCCT24630.13019078261945122No Hit
CAACAAAAGTGAGCCTGAAAGTAAAAGGATGTCTCTTGAAGAGAGAAAAG24500.12950362055122025No Hit
GTCCGGGAGCAACCAATGCCACCATAAACTTCGAAGCAGGAATTCTGGAG24010.12691354814019587No Hit
GACCTAGACTCTGCCTTGGAATGGATAAAAAACAAAAGATGCTTAACTGA23910.12638496193386436No Hit
GTAAAAGGATGTCTCTTGAAGAGAGAAAAGCAATTGGAGTAAAAATGATG23350.12342487917840789No Hit
GTCCAAGCCATGAAATGGAGAGCTGATAAAATAAAAGAACAAATTGAAAG23130.12226198952447857No Hit
CTATTGTCCAAGCCATGAAATGGAGAGCTGATAAAATAAAAGAACAAATT22920.12115195849118239No Hit
CCTTAAGGCTCAGTGCCTGGGCCCCTGCTCTTCTCTTTGTACATGCTACC22750.12025336194041883No Hit
CTTCTGTTGGATGTTTGTGAGATGTCATCGAGTGTGAGTAAATGGCTGGA22450.11866760332142427No Hit
TGATACAACCATGTCAGCTATTATGGAGCTGTTAGCTATTACTGTTTTGT22400.11840331021825852No Hit
ATTCTGGAGTGCTATGAAAGACTTTCATGGCAAAGGGCCCTTGACTACCC22350.11813901711509277No Hit
ATTCATCACAGAGCCCTTATCGGGAATGGGAACAACAGCAACAAAAAAGA22280.11776900677066071No Hit
GCGTAATGTATTGTCCCTGAGAGTATTAGTAAACGGAACATTCCTCAAAC22110.11687041021989715No Hit
GTTCAAACATTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAGC22050.11655325849609824No Hit
CAATACTCCAATGTTGCTTTGCAGTTCTTCTGCCAGTTTTTGGACGTCTT22030.11644754125483193No Hit
GGTTCAAACATTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAG21970.11613038953103302No Hit
TACTAATACTCTCAGGGACAATACATTACGCATATCCCTTCTTATTGTCA21780.11512607573900316No Hit
CCTTAATACTATTTCAGTTGGGCCATCAACATCATCTGGTTCTTCTTCTA21530.11380461022317438No Hit
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG20800.10994593091695436No Hit
GAATAAAGACCCACAACAAAAGTGAGCCTGAAAGTAAAAGGATGTCTCTT20710.10947020333125598No Hit
CATATGACCAGAGTGGAAGGCTTGTTGCTAAACTTGTTGCTACTGATGAT20690.1093644860899897No Hit
GCCCTATCCTGGCTTAGGTCTTACCTCTCTGACCGACTTCAATTTGTACA20310.10735585850592995No Hit
CCATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGACTTGCCCCAAGG20230.10693298954086473No Hit
GGACAATACATTACGCATATCCCTTCTTATTGTCAAACGGAACTTCCCTT20000.10571724126630226No Hit
TCTTCATCCTCCACTGTAAGATCATCAGTAGCAACAAGTTTAGCAACAAG19880.10508293781870445No Hit
GTCCCTGAGAGTATTAGTAAACGGAACATTCCTCAAACACCCCAATGGAT19740.10434291712984033No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGGG151.2272484E-4145.08679145
CGCACCC253.6994606E-8145.08679145
CGATAAA650.0122.76575145
AGCACGC10350.0119.676911
GCACGCA10600.0116.170982
CACGCAC10750.0114.556053
AGACGCC553.6379788E-12105.51766145
TGACGCC800.099.74717145
CCCTGCC1850.098.031624145
GGCACCC451.0875738E-896.724525145
GCCGACC451.0875738E-896.724525145
CGGTGCA406.1518404E-790.545042
AAACACG2100.082.7905965
GACGACC451.2289311E-680.603775145
ACAACGC9650.077.343768
GAAACAC2300.075.589424
CAACGCA10150.073.5337149
CTGTAAA608.00228E-872.543396145
GCAGCCC300.001931419672.543396145
GGTGCAT406.69218E-572.439863
Produced by FastQC (version 0.11.5)