FastQCFastQC Report
Thu 11 Jul 2019
HWC5JBCX2_l02_n02_SM2_9971_P2_DM2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l02_n02_SM2_9971_P2_DM2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2099636
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG83680.39854527165661097No Hit
CTACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAAT57340.2730949555065735No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT56630.2697134169922787No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA52410.24961469511858245No Hit
CATTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAGCTCTGCTT50100.23861278812136963No Hit
GATATACAGAAAGCACTAATTGGTGCCTCTATCTGCTTTTTAAAACCCAA48930.23304039366823584No Hit
GGTCAAGACCGTCTAAACAGACTAAAGAGGAAATTAGAGTCAAGAATAAA47530.2263725712456826No Hit
GTATCCATTGGGGTGTTTGAGGAATGTTCCGTTTACTAATACTCTCAGGG47330.22542002518531784No Hit
GTATGAAAAGTTCCTCAGAGAGCAACTGTCCGAAATACAGTTGGATTGAT46850.22313391464044244No Hit
CTTTTCATACAGTATGGCTCAAACCCTTCAATTCCAGCTGATGGATTCAT45820.21822830242956398No Hit
ACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGC45190.21522778233941503No Hit
GCTTTAGCACTTCCATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGA43430.20684537700820524No Hit
ATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAG42940.2045116391603116No Hit
CTATAGAACATGCTTGTAAATTCAAACATTCCAGTTTCGTTACAGTAACT39710.18912802028542092No Hit
GTAAGAAATACAGTAAAAATTGAATTTAATGTGATTCTTCTACCTCCAAA39330.18731818277072787No Hit
ATATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTCAT39290.18712767355865492No Hit
CAATATGACCACAACACAAATTGAGGTGGGTCCGGGAGCAACCAATGCCA38920.18536546334698015No Hit
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG38210.18198392483268527No Hit
GTCCTATTGTCCAAGCCATGAAATGGAGAGCTGATAAAATAAAAGAACAA36990.17617339386446032No Hit
TGACTACCCTGGTCAAGACCGTCTAAACAGACTAAAGAGGAAATTAGAGT35950.17122015435056362No Hit
GAGCCATACTGTATGAAAAGTTCCTCAGAGAGCAACTGTCCGAAATACAG34040.16212333947408028No Hit
CATTCACACAGAGCTCATAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGT33540.1597419743231684No Hit
CCCCTAGACCAAATGATGGAAGCATAACAGGGCCTTGCGAATCTGATGGA32830.15636043580887354No Hit
GTTCATAGCTGAGACCATCTGCATTTCCCGTCTCACTCCTGGCACTGAGG32810.15626518120283708No Hit
CTCTTGAAGAGAGAAAAGCAATTGGAGTAAAAATGATGAAAGTACTTCTA31830.1515977055070498No Hit
ATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGACTTGCCCCAAGGGA30630.1458824291448613No Hit
GTATGGCTCAAACCCTTCAATTCCAGCTGATGGATTCATAAATAGAAGTA29700.14145308996416522No Hit
GTGTGAATGTGATGCTTGTTTTTCGCACAAAGCACAGAGTGTTCCTAGTT29650.14121495344907403No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC29360.13983376166154513No Hit
GCCTTGGAATGGATAAAAAACAAAAGATGCTTAACTGATATACAGAAAGC29280.13945274323739926No Hit
CTTCTGACTTGCCCCAAGGGATCTCAATACTCCAATGTTGCTTTGCAGTT29260.13935748863136277No Hit
ACATTACGCATATCCCTTCTTATTGTCAAACGGAACTTCCCTTCTTTCTG28430.13540442248084905No Hit
GAGTGTTCCTAGTTTTACTTGCATTGAATAATTTCCAGGATTCAGGTACA28320.13488052214764845No Hit
GGTCATATGCATTCAATCTATGCAGAGTTGATAAGGACTTGTATCCATTG28140.13402323069332017No Hit
GTCTAGGGGTGTCCAAATAAGTCTTTGTGCACATCAATCTTATTTCCGCT27950.13311831193597365No Hit
GTTCTATAGAGATGGATTTGTATCTAACTTTGCAATGGAAATTCCTTCAT27660.13173712014844477No Hit
CTGTAAGATCATCAGTAGCAACAAGTTTAGCAACAAGCCTTCCACTCTGG26770.12749829017982164No Hit
GTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAGTAAAA26270.12511692502890978No Hit
GTCATGTACCTGAATCCTGGAAATTATTCAATGCAAGTAAAACTAGGAAC26050.12406912436250855No Hit
TGATACAACCATGTCAGCTATTATGGAGCTGTTAGCTATTACTGTTTTGT25950.12359285133232618No Hit
GCTCATAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGTGAGACGGGAAAT25580.1218306411206514No Hit
AATGAATACAAGTCCTTATCAACTCTGCATAGATTGAATGCATATGACCA25290.1204494493331225No Hit
GTATTGTCCCTGAGAGTATTAGTAAACGGAACATTCCTCAAACACCCCAA25280.12040182203010427No Hit
CTATTGTCCAAGCCATGAAATGGAGAGCTGATAAAATAAAAGAACAAATT25190.11997317630294013No Hit
CTCTTAAGGTCTGCCGCCACTGCCACTCCATGTGCTCCGTGAGATGTGTA25010.11911588484861185No Hit
TCTGTGACCAGTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTT24610.11721079272788235No Hit
GCTATGAGCTCTGTGTGAATGTGATGCTTGTTTTTCGCACAAAGCACAGA24540.11687740160675468No Hit
GTCCAAGCCATGAAATGGAGAGCTGATAAAATAAAAGAACAAATTGAAAG24480.11659163778864527No Hit
GTAGTAACAAGAGCGACCCTTGCAGGCGATATAGAGAATGAGCTGACCTG24360.11602011015242643No Hit
GTATATGCTTCTCCATATTTTATTTTGAGCAATGCATTACTGTCTGGGCC24270.11559146442526229No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC23990.11425789994075164No Hit
CTCAGGGACAATACATTACGCATATCCCTTCTTATTGTCAAACGGAACTT23360.11125737985060266No Hit
GTTCAAACATTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAGC23200.11049534300231088No Hit
CTTTACATACAGTCCCATCCCCATTCTTTGAGTTTTAGACATCGTCCGAG23010.10959042424496437No Hit
CAACAAAAGTGAGCCTGAAAGTAAAAGGATGTCTCTTGAAGAGAGAAAAG22450.10692329527594306No Hit
ATACTATACTGCTCTATTACTCAACTGCTGCTTCTAGTTTGGCTGTAACA22370.10654227685179717No Hit
GGTTCAAACATTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAG22140.10544684888237771No Hit
GCTATGAACACAGCAAAAACAATGAATGGAATGGGGAAAGGAGAAGACGT22140.10544684888237771No Hit
GTCCGGGAGCAACCAATGCCACCATAAACTTCGAAGCAGGAATTCTGGAG21550.10263683800430169No Hit
GTAAAAGGATGTCTCTTGAAGAGAGAAAAGCAATTGGAGTAAAAATGATG21270.10130327351979104No Hit
CCATAGAGGCTCTTCATTTGGGTTTTATTATCCGAATGGAACCCCCAAAC21160.10077937318659043No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGAGCT100.0071072564144.891829
GGCACCC700.0114.01195145
GCTAGCC203.8554185E-4108.82959145
GGTGCAT2450.0106.425773
CGCACCC900.0104.79886145
AGCACGC8200.0100.6862951
GCACGCA8300.099.4779362
GCCGACC301.594069E-596.73741145
CACGCAC8650.095.455093
GAAACAC5950.094.9529954
GAGCCCC502.2584572E-887.06367145
AAACACG6500.086.918525
TCCGATA259.4258814E-486.91232
GGAGGCC353.425705E-582.91778145
CAACGCA2950.081.0411769
CGGTGCA3250.080.2267462
ACGCACT10800.078.468114
ACAACGC3050.078.3840948
CGCACTT10750.077.4855045
GCGGTGC4000.074.234061
Produced by FastQC (version 0.11.5)