FastQCFastQC Report
Tue 2 Jul 2019
HWC5JBCX2_l02_n02_SM2_9932_2_P2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l02_n02_SM2_9932_2_P2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1857783
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG79660.42879066069610927No Hit
GTATAAATACTGGGACAATTGACAAAACACCGGAAGAAATAACTTCTGGA67560.36365926483340627No Hit
GTATTTATACCGTCAATATCCATGTTGGACATTTTGATTTTCAGTTCTTT55010.29610562697580933No Hit
TCATAATCCTCTGCTGTGTCCCTCCCAAAGAAGAAATTGGGGATGGTCTG49190.26477796384184804No Hit
CAGTTATAGTGTCTATTTTGTTGCTTTAATAATCGAGGTCATCATAATCC40450.21773264154101962No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT38520.20734391476291905No Hit
CTACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAAT34320.18473632281057584No Hit
ATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATGTT32700.17601625162895776No Hit
GATATACAGAAAGCACTAATTGGTGCCTCTATCTGCTTTTTAAAACCCAA32180.1732172164348581No Hit
CATTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAGCTCTGCTT30510.16422800725380735No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG30220.16266700685709795No Hit
ACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGC28520.1535163148763876No Hit
CTATAGAACATGCTTGTAAATTCAAACATTCCAGTTTCGTTACAGTAACT28310.15238593527877045No Hit
GTCTTATCATCTCTTACCATCTTGTAAAAGGTGCCTCCTGTTTTGTTGTG27560.14834886528728058No Hit
ATATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTCAT27030.14549600249329442No Hit
TTCTTATAGGGCTGAAGTAGATGGTCTTATCATCTCTTACCATCTTGTAA26770.1440964848962446No Hit
GCTTTAGCACTTCCATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGA26300.14156658770157765No Hit
ATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAG26280.14145893250180455No Hit
GTCTAAGCAGGTCTGGTTGCATTTGTGTTTGGTTTCGAAGCATCCGTTTC25460.137045069311109No Hit
GTAAGAAATACAGTAAAAATTGAATTTAATGCGATTCTTCTACCTCCAAA24970.13440751691666894No Hit
CCATATAAACAATAAAAATAGCTAGCATTAATGTTACAGCCAAACTAGAA24700.13295417171973262No Hit
GTTATAGTGTCTATTTTGTTGCTTTAATAATCGAGGTCATCATAATCCTC24190.1302089641255195No Hit
GAATAAACACCCACATTTCAAACGTATTGAGACAATCACAGTTATAGTGT23060.12412644533834145No Hit
TCTGGGGACTGTGCTTCCCGCAAAAGTGCTGATTAATGAAGGATCAAGTC23040.1240187901385684No Hit
ACCATAGAGTACTCTTCAACATTGAACCCTAGTTGAGGTATTTTGGCGTA22910.12331903134004349No Hit
GTTGTAGACGCTCTTCTTTATCTCTGTCGGGGTCTGTTTCTTTTGGGTTT22200.11949727174809975No Hit
TTTTAATGCTGATCTAGGCTTGAATTCTGTGCCTGTTAATGCAGACAAAA21750.11707502975320584No Hit
GTTCATAGCTGAGACCATCTGCATTTCCCGTCTCACTCCTGGCACTGAGG21510.11578316735592908No Hit
CATTCACACAGAGCTCATAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGT21510.11578316735592908No Hit
GCATTAAAGGTGCCAGCAGCTATCCTGTCTAAGCAGGTCTGGTTGCATTT20170.10857026897113387No Hit
GTCTACAGCAGAAGCACAGCATTTTCTTGTGAACTTCAAGCACCAGTAAA20070.10803199297226855No Hit
CTATAAGAATTACCTTTTTGAAAGAAGAGGTGAAAACAATGTACAAAACC20070.10803199297226855No Hit
TGATAAGACCATCTACTTCAGCCCTATAAGAATTACCTTTTTGAAAGAAG19950.10738606177363018No Hit
GTCCCCAGAAGATCAGGTGCGACTGGTGTTGCAATCAAAGGAGGCGGAAC19690.10598654417658038No Hit
CTATCTGCCATTGCTCTTCCTATAAATCGAATGGCTTCAGCCACTAAAGT19680.10593271657669383No Hit
GTTCTATAGAGATGGATTTGTATCTAACTTTGCAATGGAAATTCCTTCAT19330.10404875058066523No Hit
CTTCTGACTTGCCCCAAGGGATCTCAATACTCCAATGTTGCTTTGCAGTT19250.10361812978157299No Hit
TCATTAATCAGCACTTTTGCGGGAAGCACAGTCCCCAGAAGATCAGGTGC19190.10329516418225379No Hit
GCATTAATGTTACAGCCAAACTAGAAGCAGCAGTTGAGTAATAGAGCAGT19170.10318750898248073No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC19160.10313368138259421No Hit
GTAAAGAGCCATGGCTTCATACCCAACCATAGAGTACTCTTCAACATTGA18990.10221861218452316No Hit
CCTTCTACCTGTTCCTTTGCTGGAACATGGAAACCCTTGCATTTTAATGC18900.10173416378554438No Hit
GTGTGAATGTGATGCTTGTTTTTCGCACAAAGCACAGAGTGTTCCTAGTT18840.1014111981862252No Hit
GAGTAATAGAGCAGTATAGTATGGTTATCCAATCCATCATCATTTAAAGA18730.10081909458747335No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGCA100.007116313144.829671
AGCACGC5000.0117.312031
GCACGCA5050.0116.150512
CACGCAC5150.0113.90133
TCGCCCC203.8548466E-4108.833145145
GGCACCC850.0102.4312145
CGGTGCA750.096.553112
GCGGTGC1700.093.713311
ACGGTAA353.425045E-582.920494145
AAACACG3250.082.448165
ACGTGCA1150.081.860242
ATAAGCC1100.079.15138145
ACGCACT7850.076.570124
CGCACTT7800.075.206095
CAACGCA2700.075.125179
ACAACGC2700.075.1211248
TGACGCC406.639502E-572.55543145
CGCACCC300.001930143372.55543145
GAGCGTT502.3191042E-672.436287
CAGAGCG406.7009336E-572.420685
Produced by FastQC (version 0.11.5)