FastQCFastQC Report
Thu 11 Jul 2019
HWC5JBCX2_l02_n02_SM2_9932_2_P2_DM2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l02_n02_SM2_9932_2_P2_DM2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1869067
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG79920.4275930183348163No Hit
GTATAAATACTGGGACAATTGACAAAACACCGGAAGAAATAACTTCTGGA67790.36269432823970466No Hit
GTATTTATACCGTCAATATCCATGTTGGACATTTTGATTTTCAGTTCTTT55250.2956020303178003No Hit
TCATAATCCTCTGCTGTGTCCCTCCCAAAGAAGAAATTGGGGATGGTCTG49330.2639284734041102No Hit
CAGTTATAGTGTCTATTTTGTTGCTTTAATAATCGAGGTCATCATAATCC40600.21722067748240165No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT38660.2068411672775775No Hit
CTACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAAT34420.18415605219074543No Hit
ATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATGTT32790.1754351235134963No Hit
GATATACAGAAAGCACTAATTGGTGCCTCTATCTGCTTTTTAAAACCCAA32240.17249247886779873No Hit
CATTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAGCTCTGCTT30570.1635575396708625No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG30310.1621664712928964No Hit
ACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGC28600.15301752157627307No Hit
CTATAGAACATGCTTGTAAATTCAAACATTCCAGTTTCGTTACAGTAACT28450.15221498212744647No Hit
GTCTTATCATCTCTTACCATCTTGTAAAAGGTGCCTCCTGTTTTGTTGTG27650.14793477173370456No Hit
ATATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTCAT27140.1452061376076941No Hit
TTCTTATAGGGCTGAAGTAGATGGTCTTATCATCTCTTACCATCTTGTAA26860.14370806396988442No Hit
GCTTTAGCACTTCCATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGA26360.14103293247379575No Hit
ATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAG26360.14103293247379575No Hit
GTCTAAGCAGGTCTGGTTGCATTTGTGTTTGGTTTCGAAGCATCCGTTTC25530.13659221419028852No Hit
GTAAGAAATACAGTAAAAATTGAATTTAATGCGATTCTTCTACCTCCAAA25090.13423809847373047No Hit
CCATATAAACAATAAAAATAGCTAGCATTAATGTTACAGCCAAACTAGAA24760.13247251168631194No Hit
GTTATAGTGTCTATTTTGTTGCTTTAATAATCGAGGTCATCATAATCCTC24240.12969037493037971No Hit
TCTGGGGACTGTGCTTCCCGCAAAAGTGCTGATTAATGAAGGATCAAGTC23140.12380508563898458No Hit
GAATAAACACCCACATTTCAAACGTATTGAGACAATCACAGTTATAGTGT23130.12375158300906282No Hit
ACCATAGAGTACTCTTCAACATTGAACCCTAGTTGAGGTATTTTGGCGTA22960.12284203830039266No Hit
GTTGTAGACGCTCTTCTTTATCTCTGTCGGGGTCTGTTTCTTTTGGGTTT22290.11925736209563381No Hit
TTTTAATGCTGATCTAGGCTTGAATTCTGTGCCTGTTAATGCAGACAAAA21850.11690324637907577No Hit
GTTCATAGCTGAGACCATCTGCATTTCCCGTCTCACTCCTGGCACTGAGG21580.11545867537118787No Hit
CATTCACACAGAGCTCATAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGT21560.11535167011134431No Hit
GCATTAAAGGTGCCAGCAGCTATCCTGTCTAAGCAGGTCTGGTTGCATTT20200.10807531244198308No Hit
CTATAAGAATTACCTTTTTGAAAGAAGAGGTGAAAACAATGTACAAAACC20120.1076472914026089No Hit
GTCTACAGCAGAAGCACAGCATTTTCTTGTGAACTTCAAGCACCAGTAAA20090.10748678351284358No Hit
TGATAAGACCATCTACTTCAGCCCTATAAGAATTACCTTTTTGAAAGAAG20050.10727277299315648No Hit
GTCCCCAGAAGATCAGGTGCGACTGGTGTTGCAATCAAAGGAGGCGGAAC19760.10572119672542504No Hit
CTATCTGCCATTGCTCTTCCTATAAATCGAATGGCTTCAGCCACTAAAGT19720.10550718620573793No Hit
GTTCTATAGAGATGGATTTGTATCTAACTTTGCAATGGAAATTCCTTCAT19380.10368809678839763No Hit
CTTCTGACTTGCCCCAAGGGATCTCAATACTCCAATGTTGCTTTGCAGTT19310.10331357837894521No Hit
GCATTAATGTTACAGCCAAACTAGAAGCAGCAGTTGAGTAATAGAGCAGT19290.10320657311910168No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC19270.10309956785925813No Hit
TCATTAATCAGCACTTTTGCGGGAAGCACAGTCCCCAGAAGATCAGGTGC19240.1029390599694928No Hit
GTAAAGAGCCATGGCTTCATACCCAACCATAGAGTACTCTTCAACATTGA19090.1021365205206662No Hit
CCTTCTACCTGTTCCTTTGCTGGAACATGGAAACCCTTGCATTTTAATGC18960.10144098633168314No Hit
GTGTGAATGTGATGCTTGTTTTTCGCACAAAGCACAGAGTGTTCCTAGTT18950.10138748370176136No Hit
GAGTAATAGAGCAGTATAGTATGGTTATCCAATCCATCATCATTTAAAGA18770.10042443636316943No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGCC151.2267528E-4145.10144145
GGCACCC650.0133.93979145
CACGCAC5150.0129.383263
GCACGCA5100.0129.224692
AGCACGC5150.0126.5638051
GCCTCCC301.5942787E-596.73429145
ACGTGCA1350.091.198852
CGGTGCA651.6370905E-1189.135542
AGACGCC259.3619863E-487.06086145
ATAAGCC1100.085.74175145
ACGCACT8550.080.4760364
CGCACTT8650.078.710465
ACAACGC3150.073.585978
GCTACCC300.00193064372.55072145
GCGGTGC2050.070.656221
GCCATCC651.3919635E-766.969894145
CAACGCA3500.066.229149
ACTCGCC451.19000324E-464.489525145
AAACACG3100.063.084515
CGGTGCC1150.062.976192
Produced by FastQC (version 0.11.5)