FastQCFastQC Report
Tue 2 Jul 2019
HWC5JBCX2_l02_n02_SM2_9932_1_P2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l02_n02_SM2_9932_1_P2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2076940
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAAT127470.6137394436045336No Hit
CATTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAGCTCTGCTT117490.5656879832830992No Hit
GTAAGAAATACAGTAAAAATTGAATTTAATGCGATTCTTCTACCTCCAAA106790.5141698845416814No Hit
ACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGC106780.5141217367858484No Hit
ATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAG99740.48022571667934555No Hit
GCTTTAGCACTTCCATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGA96700.46558879890608296No Hit
CATTCACACAGAGCTCATAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGT85270.41055591398884894No Hit
GTGTTAAGTCATCCATGCTTTGACATTTTTCAGGAAACCTTCTATCTTAG82890.39909674810057105No Hit
GTCCTATTGTCCAAGCCATGAAATGGAGAGCTGATAAAATAAAAGAACAA81730.39351160842393135No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT70000.33703429083170433No Hit
CTTCTGACTTGCCCCAAGGGATCTCAATACTCCAATGTTGCTTTGCAGTT68620.33038990052673645No Hit
GTAGTAACAAGAGCATCATCACTTCTGTTGGATGTTTGTGAGATGTCATC66810.32167515672094527No Hit
GTTCATAGCTGAGACCATCTGCATTTCCCGTCTCACTCCTGGCACTGAGG66560.32047146282511774No Hit
GCTATGAACACAGCAAAAACAATGAATGGAATGGGGAAAGGAGAAGACGT65770.3166677901143028No Hit
ATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGACTTGCCCCAAGGGA64880.31238263984515685No Hit
GTCATGTACCTGAATCCTGGAAATTATTCAATGCAAGTAAAACTAGGAAC64250.30934933122767144No Hit
GCTCATAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGTGAGACGGGAAAT63580.30612343158685373No Hit
GTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAGTAAAA60850.29297909424441726No Hit
CTATTGTCCAAGCCATGAAATGGAGAGCTGATAAAATAAAAGAACAAATT56240.27078297880535784No Hit
GAATAAAGGGGCCAAATAAAGAGACAATAAACAGAGAGGTATCAATTTTG55890.26909780735119937No Hit
ATCAAAGGCTGTGCCCTATCCTGGCTTAGGTCTTACCTCTCTGACCGACT55010.2648608048378865No Hit
GTCCAAGCCATGAAATGGAGAGCTGATAAAATAAAAGAACAAATTGAAAG54750.2636089631862259No Hit
AGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACATTGATTACAAT52380.252197945053781No Hit
GATATACAGAAAGCACTAATTGGTGCCTCTATCTGCTTTTTAAAACCCAA50870.24492763392298283No Hit
ATATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTCAT50750.24434986085298563No Hit
ACTCTGTGCTTTGTGCGAAAAACAAGCATCACATTCACACAGAGCTCATA50550.2433869057363236No Hit
CTCCAATGTTGCTTTGCAGTTCTTCTGCCAGTTTTTGGACGTCTTCTCCT48520.23361291130220418No Hit
GGTTCAAACATTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAG47720.22976109083555615No Hit
AGTAGAAACAACGCACTTTGGTTGTTGGTATCAAAGGCTGTGCCCTATCC47620.22927961327722518No Hit
GTTCAAACATTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAGC47500.22870184020722795No Hit
CAATACTCCAATGTTGCTTTGCAGTTCTTCTGCCAGTTTTTGGACGTCTT46790.2252833495430778No Hit
ATCCTGGCTTAGGTCTTACCTCTCTGACCGACTTCAATTTGTACATGTCC44840.21589453715562318No Hit
GATTCTGGCTGAGAGAAAAATGAGAAAATGTGTGAGCTTTCATGAAGCAT44560.21454639999229635No Hit
GTGTGAATGTGATGCTTGTTTTTCGCACAAAGCACAGAGTGTTCCTAGTT43850.2111279093281462No Hit
TTCACACAGAGCTCATAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGTGA43070.20737238437316435No Hit
CCATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGACTTGCCCCAAGG42570.20496499658150935No Hit
GCTATGAGCTCTGTGTGAATGTGATGCTTGTTTTTCGCACAAAGCACAGA39590.19061696534324535No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG39050.18801698652825793No Hit
ATTTAAATGTCCTATTGTCCAAGCCATGAAATGGAGAGCTGATAAAATAA36710.17675041166331237No Hit
GCGCTACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTC36490.17569116103498414No Hit
TTGCAATTCCTTCCCCATTCTTCTGACTTGCCCCAAGGGATCTCAATACT36020.17342821651082843No Hit
GAAATGGAGAGCTGATAAAATAAAAGAACAAATTGAAAGAATCTGGAATG34530.16625420089169643No Hit
CTTCCATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGACTTGCCCCA34100.1641838473908731No Hit
CCTTAAGGCTCAGTGCCTGGGCCCCTGCTCTTCTCTTTGTACATGCTACC34020.1637986653442083No Hit
AAATTATTCAATGCAAGTAAAACTAGGAACACTCTGTGCTTTGTGCGAAA33580.1616801640875519No Hit
ATCTTAGGTAGTTTAGGTTGGTATAATTTCATCAGGTTTCATGGATAGGT32880.15830982117923484No Hit
TCTCAATACTCCAATGTTGCTTTGCAGTTCTTCTGCCAGTTTTTGGACGT32220.1551320692942502No Hit
GTTTATTGTCTCTTTATTTGGCCCCTTTATTCTTATTTTCATGTTTACTC31820.15320615906092616No Hit
ATTACTATGTGGTCACTCAATACTTCCATGTTGTTAGAGAGTACTTCCTT31330.15084691902510425No Hit
GCCCCAAGGGATCTCAATACTCCAATGTTGCTTTGCAGTTCTTCTGCCAG31240.15041358922260634No Hit
CCTATAATGTTTGAACCATTCCAGATTCTTTCAATTTGTTCTTTTATTTT30940.1489691565476133No Hit
ATCTGGAATGGTTCAAACATTATAGGTATTTCTTCACAAGAGCTGAATTT30520.14694695080262307No Hit
GCCTTGGAATGGATAAAAAACAAAAGATGCTTAACTGATATACAGAAAGC29670.14285439155680954No Hit
GTGCCAGGAGTGAGACGGGAAATGCAGATGGTCTCAGCTATGAACACAGC29650.14275809604514333No Hit
TTCATGAAGCATTTGAAATAGCAGAAGGCCATGAAAGCTCAGCGCTACTA29300.1410729245909848No Hit
CATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGACTTGCCCCAAGGG29280.14097662907931863No Hit
ATTTTGATCTGTGTTAAGTCATCCATGCTTTGACATTTTTCAGGAAACCT28900.1391470143576608No Hit
TTGTTAGAGAGTACTTCCTTCATTGCTTCCTTAGCCTGAATTTCTTTTTG28360.13654703554267336No Hit
CTCTGTGCTTTGTGCGAAAAACAAGCATCACATTCACACAGAGCTCATAG27940.13452482979768313No Hit
CCTTTATTCTTATTTTCATGTTTACTCTTCTTTTTATTTGATTTAAATGT27670.13322484039018942No Hit
CCCCAAGGGATCTCAATACTCCAATGTTGCTTTGCAGTTCTTCTGCCAGT27470.13226188527352742No Hit
TTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAGCTCTGCTTTA27430.132069294250195No Hit
GGGGAAAGGAGAAGACGTCCAAAAACTGGCAGAAGAACTGCAAAGCAACA27390.1318767032268626No Hit
ACTAGGAACACTCTGTGCTTTGTGCGAAAAACAAGCATCACATTCACACA26970.1298544974818724No Hit
CTATAGAACATGCTTGTAAATTCAAACATTCCAGTTTCGTTACAGTAACT26760.12884339460937727No Hit
CCTTTCCCCATTCCATTCATTGTTTTTGCTGTGTTCATAGCTGAGACCAT26700.12855450807437865No Hit
TAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGTGAGACGGGAAATGCAGA26640.12826562153938006No Hit
CAATTACTATGTGGTCACTCAATACTTCCATGTTGTTAGAGAGTACTTCC26590.12802488276021456No Hit
GCCCTATCCTGGCTTAGGTCTTACCTCTCTGACCGACTTCAATTTGTACA26590.12802488276021456No Hit
CTTCACAAGAGCTGAATTTCCCATAGAGCTCTGCTTTAGCACTTCCATTA26280.12653230232938842No Hit
CCATAGAGCTCTGCTTTAGCACTTCCATTACATCCTTTGCAATTCCTTCC25800.12422121004939961No Hit
GCACAGAGTGTTCCTAGTTTTACTTGCATTGAATAATTTCCAGGATTCAG25670.12359528922356929No Hit
TGTCCAAAGTGAGTCATCCAGTCATACAACAGTAAGTCAAGGAGTTCCTT25010.12041753733858465No Hit
AAGAAATACAGTAAAAATTGAATTTAATGCGATTCTTCTACCTCCAAAAC24980.12027309407108536No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA24440.11767311525609792No Hit
AGCAGGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT24290.1169508989186014No Hit
GGAGTATTGAGATCCCTTGGGGCAAGTCAGAAGAATGGGGAAGGAATTGC24070.1158916482902732No Hit
GAGTGTTCCTAGTTTTACTTGCATTGAATAATTTCCAGGATTCAGGTACA23820.11468795439444567No Hit
GTCATACAACAGTAAGTCAAGGAGTTCCTTAAGGCTCAGTGCCTGGGCCC23440.11285833967278784No Hit
GTAGAAACAACAGCAACAAAAAAGAAGGGCCTGATTCTGGCTGAGAGAAA23360.11247315762612305No Hit
CTAGGAACACTCTGTGCTTTGTGCGAAAAACAAGCATCACATTCACACAG23100.11122131597446243No Hit
CTTTGGTTGTTGGTATCAAAGGCTGTGCCCTATCCTGGCTTAGGTCTTAC23060.11102872495113003No Hit
ACATTGGAGTATTGAGATCCCTTGGGGCAAGTCAGAAGAATGGGGAAGGA22970.11059539514863212No Hit
CTTCTGTTGGATGTTTGTGAGATGTCATCGAGTGTGAGTAAATGGCTGGA22840.10996947432280182No Hit
GTTTAGTACTTACAAACTATTTTGATCTGTGTTAAGTCATCCATGCTTTG22660.109102814717806No Hit
GTTTTTGCTGTGTTCATAGCTGAGACCATCTGCATTTCCCGTCTCACTCC22620.10891022369447359No Hit
ACCCTTAGGTGCTGTAATGAGGAAGCATGGGTTTCCATTGCTATGCTGAT22620.10891022369447359No Hit
GACAATAAACAGAGAGGTATCAATTTTGAGACACAGTTACCAAAAAGAAA22170.10674357468198409No Hit
GATTACAATTTGCTTAAATGCATAGTAAGAAATACAGTAAAAATTGAATT22010.10597321058865446No Hit
TCCCCATTCCATTCATTGTTTTTGCTGTGTTCATAGCTGAGACCATCTGC21940.10563617629782275No Hit
GGTCTCAGCTATGAACACAGCAAAAACAATGAATGGAATGGGGAAAGGAG21660.10428803913449594No Hit
GCCTGATTCTGGCTGAGAGAAAAATGAGAAAATGTGTGAGCTTTCATGAA21610.10404730035533044No Hit
ACAATAAACAGAGAGGTATCAATTTTGAGACACAGTTACCAAAAAGAAAT21300.10255471992450431No Hit
ATGAAATGGAGAGCTGATAAAATAAAAGAACAAATTGAAAGAATCTGGAA21170.10192879909867401No Hit
GCTTAAATGCATAGTAAGAAATACAGTAAAAATTGAATTTAATGCGATTC21070.101447321540343No Hit
AAAAAGAAGGGCCTGATTCTGGCTGAGAGAAAAATGAGAAAATGTGTGAG20940.1008214007145127No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACGC8550.0116.88971
GTGACCC256.441289E-6116.09127145
GCACGCA8950.0111.665582
GCAGCCC850.0110.9696145
CACGCAC9150.0109.22483
ATAAGCC750.0106.417145
CGTTACC352.7498936E-7103.652916145
GCGAGAA1300.0100.4636145
GGCACCC650.0100.4636145
CGACGAA950.099.28858145
CCCTGCC3300.096.74273145
TGACGCC607.2759576E-1296.74273145
CGCACCC301.5936303E-596.74273145
GTCGCCC451.0863005E-896.74272145
GCCGACC406.0912134E-790.696304145
CGGTGCG750.086.911235
AACAACG21300.079.9143457
ACAACGC22350.076.163658
TAGTAAC23550.074.419672
CAACGCA22950.074.172449
Produced by FastQC (version 0.11.5)