FastQCFastQC Report
Thu 11 Jul 2019
HWC5JBCX2_l02_n02_SM2_9932_1_P2_DM2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l02_n02_SM2_9932_1_P2_DM2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2087785
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAAT127840.6123235869593852No Hit
CATTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAGCTCTGCTT117810.5642822417059228No Hit
GTAAGAAATACAGTAAAAATTGAATTTAATGCGATTCTTCTACCTCCAAA107230.5136065255761488No Hit
ACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGC107090.5129359584439969No Hit
ATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAG100060.4792639088795063No Hit
GCTTTAGCACTTCCATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGA96980.4645114319721619No Hit
CATTCACACAGAGCTCATAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGT85560.40981231304947585No Hit
GTGTTAAGTCATCCATGCTTTGACATTTTTCAGGAAACCTTCTATCTTAG83100.39802949058451903No Hit
GTCCTATTGTCCAAGCCATGAAATGGAGAGCTGATAAAATAAAAGAACAA82010.3928086464841926No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT70280.33662470034031283No Hit
CTTCTGACTTGCCCCAAGGGATCTCAATACTCCAATGTTGCTTTGCAGTT68820.3296316431050132No Hit
GTAGTAACAAGAGCATCATCACTTCTGTTGGATGTTTGTGAGATGTCATC66970.32077057743014725No Hit
GTTCATAGCTGAGACCATCTGCATTTCCCGTCTCACTCCTGGCACTGAGG66700.31947734081813983No Hit
GCTATGAACACAGCAAAAACAATGAATGGAATGGGGAAAGGAGAAGACGT65960.3159329145481934No Hit
ATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGACTTGCCCCAAGGGA65040.3115263305369087No Hit
GTCATGTACCTGAATCCTGGAAATTATTCAATGCAAGTAAAACTAGGAAC64500.3089398573128938No Hit
GCTCATAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGTGAGACGGGAAAT63810.3056349193044303No Hit
GTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAGTAAAA61040.2923672696182797No Hit
CTATTGTCCAAGCCATGAAATGGAGAGCTGATAAAATAAAAGAACAAATT56440.2703343495618562No Hit
GAATAAAGGGGCCAAATAAAGAGACAATAAACAGAGAGGTATCAATTTTG56080.2686100340791796No Hit
ATCAAAGGCTGTGCCCTATCCTGGCTTAGGTCTTACCTCTCTGACCGACT55160.2642034500678949No Hit
GTCCAAGCCATGAAATGGAGAGCTGATAAAATAAAAGAACAAATTGAAAG54880.26286231580359093No Hit
AGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACATTGATTACAAT52500.2514626745570066No Hit
GATATACAGAAAGCACTAATTGGTGCCTCTATCTGCTTTTTAAAACCCAA51010.24432592436481726No Hit
ATATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTCAT50940.24399064079874125No Hit
ACTCTGTGCTTTGTGCGAAAAACAAGCATCACATTCACACAGAGCTCATA50680.24274530183903037No Hit
CTCCAATGTTGCTTTGCAGTTCTTCTGCCAGTTTTTGGACGTCTTCTCCT48650.2330220784228261No Hit
GGTTCAAACATTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAG47820.22904657328221056No Hit
AGTAGAAACAACGCACTTTGGTTGTTGGTATCAAAGGCTGTGCCCTATCC47740.22866339206383798No Hit
GTTCAAACATTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAGC47700.2284718014546517No Hit
CAATACTCCAATGTTGCTTTGCAGTTCTTCTGCCAGTTTTTGGACGTCTT47030.22526265875078133No Hit
ATCCTGGCTTAGGTCTTACCTCTCTGACCGACTTCAATTTGTACATGTCC44990.2154915376822805No Hit
GATTCTGGCTGAGAGAAAAATGAGAAAATGTGTGAGCTTTCATGAAGCAT44730.21424619872256961No Hit
GTGTGAATGTGATGCTTGTTTTTCGCACAAAGCACAGAGTGTTCCTAGTT43990.21070177245262325No Hit
TTCACACAGAGCTCATAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGTGA43150.20667836965971112No Hit
CCATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGACTTGCCCCAAGG42720.20461877061095848No Hit
GCTATGAGCTCTGTGTGAATGTGATGCTTGTTTTTCGCACAAAGCACAGA39740.19034527022657985No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG39170.18761510404567522No Hit
ATTTAAATGTCCTATTGTCCAAGCCATGAAATGGAGAGCTGATAAAATAA36870.17659864401746347No Hit
GCGCTACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTC36530.17497012383938002No Hit
TTGCAATTCCTTCCCCATTCTTCTGACTTGCCCCAAGGGATCTCAATACT36070.17276683183373767No Hit
GAAATGGAGAGCTGATAAAATAAAAGAACAAATTGAAAGAATCTGGAATG34610.16577377459843806No Hit
CTTCCATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGACTTGCCCCA34170.16366627789738886No Hit
CCTTAAGGCTCAGTGCCTGGGCCCCTGCTCTTCTCTTTGTACATGCTACC34150.16357048259279572No Hit
AAATTATTCAATGCAAGTAAAACTAGGAACACTCTGTGCTTTGTGCGAAA33670.16127139528256024No Hit
ATCTTAGGTAGTTTAGGTTGGTATAATTTCATCAGGTTTCATGGATAGGT32970.15791855962180013No Hit
TCTCAATACTCCAATGTTGCTTTGCAGTTCTTCTGCCAGTTTTTGGACGT32280.1546136216133366No Hit
GTTTATTGTCTCTTTATTTGGCCCCTTTATTCTTATTTTCATGTTTACTC31870.15264981786917714No Hit
ATTACTATGTGGTCACTCAATACTTCCATGTTGTTAGAGAGTACTTCCTT31460.15068601412501764No Hit
GCCCCAAGGGATCTCAATACTCCAATGTTGCTTTGCAGTTCTTCTGCCAG31320.15001544699286565No Hit
CCTATAATGTTTGAACCATTCCAGATTCTTTCAATTTGTTCTTTTATTTT31030.14862641507626503No Hit
ATCTGGAATGGTTCAAACATTATAGGTATTTCTTCACAAGAGCTGAATTT30610.146614713679809No Hit
GCCTTGGAATGGATAAAAAACAAAAGATGCTTAACTGATATACAGAAAGC29750.14249551558230372No Hit
GTGCCAGGAGTGAGACGGGAAATGCAGATGGTCTCAGCTATGAACACAGC29710.14230392497311745No Hit
TTCATGAAGCATTTGAAATAGCAGAAGGCCATGAAAGCTCAGCGCTACTA29390.14077120009962713No Hit
CATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGACTTGCCCCAAGGG29330.14048381418584768No Hit
ATTTTGATCTGTGTTAAGTCATCCATGCTTTGACATTTTTCAGGAAACCT28970.13875949870317109No Hit
TTGTTAGAGAGTACTTCCTTCATTGCTTCCTTAGCCTGAATTTCTTTTTG28440.1362209231314527No Hit
CTCTGTGCTTTGTGCGAAAAACAAGCATCACATTCACACAGAGCTCATAG27990.13406552877810696No Hit
CCTTTATTCTTATTTTCATGTTTACTCTTCTTTTTATTTGATTTAAATGT27740.13286808747069262No Hit
CCCCAAGGGATCTCAATACTCCAATGTTGCTTTGCAGTTCTTCTGCCAGT27540.13191013442476118No Hit
TTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAGCTCTGCTTTA27500.13171854381557488No Hit
GGGGAAAGGAGAAGACGTCCAAAAACTGGCAGAAGAACTGCAAAGCAACA27430.13138326024949887No Hit
ACTAGGAACACTCTGTGCTTTGTGCGAAAAACAAGCATCACATTCACACA27010.1293715588530428No Hit
CTATAGAACATGCTTGTAAATTCAAACATTCCAGTTTCGTTACAGTAACT26800.1283657081548148No Hit
CCTTTCCCCATTCCATTCATTGTTTTTGCTGTGTTCATAGCTGAGACCAT26780.12826991285022166No Hit
CAATTACTATGTGGTCACTCAATACTTCCATGTTGTTAGAGAGTACTTCC26690.12783883397955248No Hit
TAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGTGAGACGGGAAATGCAGA26680.1277909363272559No Hit
GCCCTATCCTGGCTTAGGTCTTACCTCTCTGACCGACTTCAATTTGTACA26650.1276472433703662No Hit
CTTCACAAGAGCTGAATTTCCCATAGAGCTCTGCTTTAGCACTTCCATTA26400.1264498020629519No Hit
CCATAGAGCTCTGCTTTAGCACTTCCATTACATCCTTTGCAATTCCTTCC25820.12367173822975067No Hit
GCACAGAGTGTTCCTAGTTTTACTTGCATTGAATAATTTCCAGGATTCAG25750.12333645466367466No Hit
TGTCCAAAGTGAGTCATCCAGTCATACAACAGTAAGTCAAGGAGTTCCTT25050.11998361900291457No Hit
AAGAAATACAGTAAAAATTGAATTTAATGCGATTCTTCTACCTCCAAAAC25040.119935721350618No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA24520.11744504343119622No Hit
AGCAGGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT24340.1165828856898579No Hit
GGAGTATTGAGATCCCTTGGGGCAAGTCAGAAGAATGGGGAAGGAATTGC24130.11557703499162988No Hit
GAGTGTTCCTAGTTTTACTTGCATTGAATAATTTCCAGGATTCAGGTACA23890.11442749133651214No Hit
GTCATACAACAGTAAGTCAAGGAGTTCCTTAAGGCTCAGTGCCTGGGCCC23490.11251158524464924No Hit
GTAGAAACAACAGCAACAAAAAAGAAGGGCCTGATTCTGGCTGAGAGAAA23440.11227209698316638No Hit
CTTTGGTTGTTGGTATCAAAGGCTGTGCCCTATCCTGGCTTAGGTCTTAC23190.11107465567575205No Hit
CTAGGAACACTCTGTGCTTTGTGCGAAAAACAAGCATCACATTCACACAG23180.11102675802345546No Hit
ACATTGGAGTATTGAGATCCCTTGGGGCAAGTCAGAAGAATGGGGAAGGA23010.11021249793441373No Hit
CTTCTGTTGGATGTTTGTGAGATGTCATCGAGTGTGAGTAAATGGCTGGA22910.10973352141144802No Hit
GTTTAGTACTTACAAACTATTTTGATCTGTGTTAAGTCATCCATGCTTTG22690.10867977306092341No Hit
GTTTTTGCTGTGTTCATAGCTGAGACCATCTGCATTTCCCGTCTCACTCC22680.10863187540862684No Hit
ACCCTTAGGTGCTGTAATGAGGAAGCATGGGTTTCCATTGCTATGCTGAT22660.1085360801040337No Hit
GACAATAAACAGAGAGGTATCAATTTTGAGACACAGTTACCAAAAAGAAA22200.10633278809839136No Hit
GATTACAATTTGCTTAAATGCATAGTAAGAAATACAGTAAAAATTGAATT22070.10571011861853592No Hit
TCCCCATTCCATTCATTGTTTTTGCTGTGTTCATAGCTGAGACCATCTGC22000.10537483505245991No Hit
GGTCTCAGCTATGAACACAGCAAAAACAATGAATGGAATGGGGAAAGGAG21740.104129496092749No Hit
GCCTGATTCTGGCTGAGAGAAAAATGAGAAAATGTGTGAGCTTTCATGAA21660.10374631487437642No Hit
ACAATAAACAGAGAGGTATCAATTTTGAGACACAGTTACCAAAAAGAAAT21360.10230938530547924No Hit
ATGAAATGGAGAGCTGATAAAATAAAAGAACAAATTGAAAGAATCTGGAA21210.10159092052103066No Hit
GCTTAAATGCATAGTAAGAAATACAGTAAAAATTGAATTTAATGCGATTC21100.10106404634576836No Hit
AAAAAGAAGGGCCTGATTCTGGCTGAGAGAAAAATGAGAAAATGTGTGAG20990.10053717217050606No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGA100.007112963144.852895
CCCTGCC2900.0115.067986145
AGCACGC8550.0107.573081
TGACGCC700.0103.63265145
GCACGCA8950.0103.5769962
CACGCAC9100.0101.8745653
CGGTGCA650.0100.277972
GGCACCC900.096.72381145
CTAGGCC259.366173E-487.05142145
GCGAGAA1200.084.63333145
GCAGCCC801.8189894E-1281.61071145
AGCCACC554.3788532E-879.137665145
CGACGAA1200.078.5881145
GGTGCAT851.8189894E-1276.686833
AACACGT3800.076.2456746
GAAACAC9000.074.035924
AAACACG9200.073.213695
GCTGCCC1400.072.542854145
GGTCACC502.2990334E-672.542854145
GCCGACC406.645397E-572.542854145
Produced by FastQC (version 0.11.5)