FastQCFastQC Report
Tue 2 Jul 2019
HWC5JBCX2_l02_n02_SM2_9906_P2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l02_n02_SM2_9906_P2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1964690
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT98840.5030819111411978No Hit
CTACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAAT56320.28666099995419125No Hit
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG54630.27805913401096355No Hit
CATTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAGCTCTGCTT54440.2770920603250385No Hit
GATATACAGAAAGCACTAATTGGTGCCTCTATCTGCTTTTTAAAACCCAA51330.26126259104489763No Hit
ATATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTCAT49890.2539331904778871No Hit
ACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGC48500.24685828298611992No Hit
ATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAG44620.22710962034723034No Hit
GCTTTAGCACTTCCATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGA40770.20751365355348683No Hit
GTCCTATTGTCCAAGCCATGAAATGGAGAGCTGATAAAATAAAAGAACAA39990.20354356157968942No Hit
CATTCACACAGAGCTCATAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGT38260.194738101176267No Hit
GTTCATAGCTGAGACCATCTGCATTTCCCGTCTCACTCCTGGCACTGAGG36720.18689971445876957No Hit
GTGTGAATGTGATGCTTGTTTTTCGCACAAAGCACAGAGTGTTCCTAGTT32910.16750734212522078No Hit
CTTCTGACTTGCCCCAAGGGATCTCAATACTCCAATGTTGCTTTGCAGTT32230.16404623630191023No Hit
GTAAGAAATACAGTAAAAATTGAATTTAATGTGATTCTTCTACCTCCAAA31460.16012704294316152No Hit
CTATAGAACATGCTTGTAAATTCAAACATTCCAGTTTCGTTACAGTAACT28820.1466898085703088No Hit
GCTATGAACACAGCAAAAACAATGAATGGAATGGGGAAAGGAGAAGACGT28290.14399218197272853No Hit
GTCATGTACCTGAATCCTGGAAATTATTCAATGCAAGTAAAACTAGGAAC28180.143432297207193No Hit
GCTATGAGCTCTGTGTGAATGTGATGCTTGTTTTTCGCACAAAGCACAGA28130.14317780413194955No Hit
GTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAGTAAAA27920.1421089332159272No Hit
GTATAAATACTGGGACAATTGACAAAACACCGGAAGAAATAACTTCTGGA27740.14119275814505086No Hit
ATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGACTTGCCCCAAGGGA27630.14063287337951536No Hit
AGCAGGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT27540.1401747858440772No Hit
GCTGTAGACATAGGAAACGGATGCTTCGAAACCAAACACAAATGCAACCA26960.13722266617125348No Hit
GCTCATAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGTGAGACGGGAAAT26740.13610289664018244No Hit
GCCTTGGAATGGATAAAAAACAAAAGATGCTTAACTGATATACAGAAAGC26670.13574660633484162No Hit
CTATTGTCCAAGCCATGAAATGGAGAGCTGATAAAATAAAAGAACAAATT26480.13477953264891662No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC25920.13192921020619028No Hit
GTATAGATCTGTTCCTTTTGGTTCTCCAGCTATGAGTTCCAGACACTGCA25810.13136932544065477No Hit
CTCCAATGTTGCTTTGCAGTTCTTCTGCCAGTTTTTGGACGTCTTCTCCT24290.12363273595325472No Hit
GTCCAAGCCATGAAATGGAGAGCTGATAAAATAAAAGAACAAATTGAAAG24020.12225847334694023No Hit
ATCCAATGATGACCAATAACCCCATGGACATCTTCGGAGCTTATGTGTAC23930.12180038581150207No Hit
TCATAATCCTCTGCTGTGTCCCTCCCAAAGAAGAAATTGGGGATGGTCTG23600.12012073151489548No Hit
ACTCTGTGCTTTGTGCGAAAAACAAGCATCACATTCACACAGAGCTCATA22810.1160997409260494No Hit
CCTAAACTCTGCCTTGGAATGGATAAAAAACAAAAGATGCTTAACTGATA21950.11172246003186254No Hit
ATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATGTT21880.11136616972652175No Hit
CAGTTATAGTGTCTATTTTGTTGCTTTAATAATCGAGGTCATCATAATCC20730.10551282899592303No Hit
GCACAGAGTGTTCCTAGTTTTACTTGCATTGAATAATTTCCAGGATTCAG20540.10454575530999802No Hit
GGTTCAAACATTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAG20370.10368047885417038No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG20360.10362958023912168No Hit
GATCTATACTAAATACTGATCAGAGGAACATGATTCTTGAAGAACAATGC19970.10164453425222299No Hit
GTTCAAACATTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAGC19960.10159363563717431No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTTTATCACATGTAT19940.10149183840707694No Hit
CAATACTCCAATGTTGCTTTGCAGTTCTTCTGCCAGTTTTTGGACGTCTT19860.10108464948668747No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCACCC306.475602E-10145.10478145
GGCACCC850.0128.03363145
AGCACGC14900.0108.883291
GCACGCA15200.0106.7342762
CACGCAC15400.0105.823813
CAGCGCC352.750876E-7103.64628145
GCCGACC352.750876E-7103.64628145
ACTCGCC301.5941161E-596.73652145
CGGTGCA900.096.569112
ACGTGCA2900.089.909172
CTACGCC259.361203E-487.06287145
GAAACAC6150.085.9814
TCGCCGG608.258212E-1084.644455145
CGATAAA353.425806E-582.91702145
ACAACGC5600.081.5030068
AAACACG6600.081.216185
ACGCACT21350.081.087664
CGCACTT21300.080.5978555
AACACGT2700.080.490656
GCGGTGC2350.080.131811
Produced by FastQC (version 0.11.5)