FastQCFastQC Report
Thu 11 Jul 2019
HWC5JBCX2_l02_n02_SM2_9906_P2_DM2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l02_n02_SM2_9906_P2_DM2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1976797
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT99240.5020242341525204No Hit
CTACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAAT56530.2859676537348044No Hit
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG54930.27787375233774636No Hit
CATTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAGCTCTGCTT54630.276356145825798No Hit
GATATACAGAAAGCACTAATTGGTGCCTCTATCTGCTTTTTAAAACCCAA51580.26092714628765623No Hit
ATATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTCAT50060.2532379399604512No Hit
ACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGC48640.2460546024705622No Hit
ATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAG44800.22662923911762312No Hit
GCTTTAGCACTTCCATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGA40890.2068497675785627No Hit
GTCCTATTGTCCAAGCCATGAAATGGAGAGCTGATAAAATAAAAGAACAA40100.20285340376376532No Hit
CATTCACACAGAGCTCATAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGT38420.19435480729685448No Hit
GTTCATAGCTGAGACCATCTGCATTTCCCGTCTCACTCCTGGCACTGAGG36850.1864126665509913No Hit
GTGTGAATGTGATGCTTGTTTTTCGCACAAAGCACAGAGTGTTCCTAGTT33020.16703789008178382No Hit
CTTCTGACTTGCCCCAAGGGATCTCAATACTCCAATGTTGCTTTGCAGTT32330.16354739510430258No Hit
GTAAGAAATACAGTAAAAATTGAATTTAATGTGATTCTTCTACCTCCAAA31590.15980396570816324No Hit
CTATAGAACATGCTTGTAAATTCAAACATTCCAGTTTCGTTACAGTAACT28980.14660078905421245No Hit
GCTATGAACACAGCAAAAACAATGAATGGAATGGGGAAAGGAGAAGACGT28380.14356557603031572No Hit
GTCATGTACCTGAATCCTGGAAATTATTCAATGCAAGTAAAACTAGGAAC28340.14336322849538924No Hit
GCTATGAGCTCTGTGTGAATGTGATGCTTGTTTTTCGCACAAAGCACAGA28310.14321146784419442No Hit
GTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAGTAAAA28010.14169386133224604No Hit
GTATAAATACTGGGACAATTGACAAAACACCGGAAGAAATAACTTCTGGA27830.14078329742507703No Hit
ATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGACTTGCCCCAAGGGA27750.14037860235522412No Hit
AGCAGGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT27670.13997390728537124No Hit
GCTGTAGACATAGGAAACGGATGCTTCGAAACCAAACACAAATGCAACCA27020.13668575984281645No Hit
GCTCATAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGTGAGACGGGAAAT26910.1361293041217687No Hit
GCCTTGGAATGGATAAAAAACAAAAGATGCTTAACTGATATACAGAAAGC26730.13521874021459968No Hit
CTATTGTCCAAGCCATGAAATGGAGAGCTGATAAAATAAAAGAACAAATT26560.13435876319116227No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC26030.13167765835338682No Hit
GTATAGATCTGTTCCTTTTGGTTCTCCAGCTATGAGTTCCAGACACTGCA25870.13086826821368103No Hit
CTCCAATGTTGCTTTGCAGTTCTTCTGCCAGTTTTTGGACGTCTTCTCCT24440.12363434384006046No Hit
GTCCAAGCCATGAAATGGAGAGCTGATAAAATAAAAGAACAAATTGAAAG24120.12201556356064888No Hit
ATCCAATGATGACCAATAACCCCATGGACATCTTCGGAGCTTATGTGTAC24000.12140852095586951No Hit
TCATAATCCTCTGCTGTGTCCCTCCCAAAGAAGAAATTGGGGATGGTCTG23700.11989091444392114No Hit
ACTCTGTGCTTTGTGCGAAAAACAAGCATCACATTCACACAGAGCTCATA22870.11569220309419732No Hit
CCTAAACTCTGCCTTGGAATGGATAAAAAACAAAAGATGCTTAACTGATA22060.11159466551193674No Hit
ATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATGTT21990.11124055732581543No Hit
CAGTTATAGTGTCTATTTTGTTGCTTTAATAATCGAGGTCATCATAATCC20770.10506895751055875No Hit
GCACAGAGTGTTCCTAGTTTTACTTGCATTGAATAATTTCCAGGATTCAG20620.10431015425458455No Hit
GGTTCAAACATTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAG20450.10345017723114715No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG20420.10329841657995233No Hit
GATCTATACTAAATACTGATCAGAGGAACATGATTCTTGAAGAACAATGC20070.10152787564934589No Hit
GTTCAAACATTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAGC20020.10127494123068781No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTTTATCACATGTAT19990.10112318057949299No Hit
CAATACTCCAATGTTGCTTTGCAGTTCTTCTGCCAGTTTTTGGACGTCTT19910.10071848550964009No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCACC100.007076587145.10132145
GCCGACC256.4440283E-6116.08106145
CGGTGCA1250.0115.88142
AGCACGC16450.0115.3530651
GCACGCA16600.0113.874412
CACGCAC16700.0113.626233
GGTGCAT1350.0107.29763
AACGGCC501.9463187E-10101.57093145
GAAACAC6200.099.296054
GGCACCC950.099.279854145
CGCACCC451.0868462E-896.734215145
ACGCACT21700.091.786264
GCTGCCC950.091.64294145
AAACACG6750.091.2075655
CGCACTT22100.090.127255
GCGGTGC3050.083.1116641
GAGCGTT755.638867E-1177.2562264
GCGAGAA809.822543E-1172.55066145
CGACGAA809.822543E-1172.55066145
CGTTACC300.001930665372.55066145
Produced by FastQC (version 0.11.5)