FastQCFastQC Report
Tue 2 Jul 2019
HWC5JBCX2_l02_n02_SM2_9861_P2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l02_n02_SM2_9861_P2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1635832
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT73820.4512688344524377No Hit
CTATAGAACATGCTTGTAAATTCAAACATTCCAGTTTCGTTACAGTAACT35160.21493649714640622No Hit
CATTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAGCTCTGCTT34680.21200221049594337No Hit
CTACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAAT34200.20906792384548048No Hit
GATATACAGAAAGCACTAATTGGTGCCTCTATCTGCTTTTTAAAACCCAA34040.20808982829532618No Hit
ATATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTCAT33500.20478875581355543No Hit
ACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGC30980.18938375089862527No Hit
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG28420.17373422209615658No Hit
GTAAGAAATACAGTAAAAATTGAATTTAATGCGATTCTTCTACCTCCAAA28280.17287838848977158No Hit
ATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAG27580.16859922045784653No Hit
GCTTTAGCACTTCCATTACATCCTTTGCAATTCCTTCTCCATTCTTCTGA27320.1670098151888458No Hit
GTCCTATTGTCCAAGCCATGAAATGGAGAGCTGATAAAATAAAAGAACAA24010.14677546349502882No Hit
GTTCATAGCTGAGACCATCTGCATTTCCCGTCTCACTCCTGGCACTGAGG23730.14506379628225882No Hit
CATTCACACAGAGCTCATAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGT22840.13962313978452556No Hit
AGCAGGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT21470.1312481966363294No Hit
GTGTGAATGTGATGCTTGTTTTTCGCACAAAGCACAGAGTGTTCCTAGTT21300.13020897011429045No Hit
GTTCTATAGAGATGGATTTGTATCTAACTTTGCAATGGAAATTCCTTCAT21010.1284361719296358No Hit
GTATAAATACTGGGACAATTGACAAAACACCGGAAGAAATAACTTCTGGA19930.12183402696609431No Hit
GCCTTGGAATGGATAAAAAACAAAAGATGCTTAACTGATATACAGAAAGC19530.11938878809070858No Hit
CTTCTGACTTGCCCCAAGGGATCTCAATACTCCAATGTTGCTTTGCAGTT19310.11804390670924643No Hit
GTCATGTACCTGAATCCTGGAAATTATTCAATGCAAGTAAAACTAGGAAC17990.1099746184204735No Hit
ATTACATCCTTTGCAATTCCTTCTCCATTCTTCTGACTTGCCCCAAGGGA17990.1099746184204735No Hit
GCTATGAACACAGCAAAAACAATGAATGGAATGGGAAGAGGAGAAGACGT17940.10966896356105027No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC17490.10691806982624133No Hit
GCTATGAGCTCTGTGTGAATGTGATGCTTGTTTTTCGCACAAAGCACAGA17480.10685693885435668No Hit
GTCATAATGGGAGCCAATGACGTAAGTGAATTAGAATCACAAGCACAACT17220.10526753358535594No Hit
GTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAGTAAAA17120.10465622386650952No Hit
GCTCATAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGTGAGACGGGAAAT16690.10202759207546985No Hit
GAATAGAGGAGACCCTTTCCTTACTTCGATGAAATTTTCTCCTCCTCCCT16470.1006827106940077No Hit
GTATTTACACAGTCTCTCCAAAGTTTCCATAGGAAATTTTCGTTAGCTTT16390.10019366291893055No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG16360.10001027000327663No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCCGG100.007078207145.08945145
AGCACGC9800.0131.551471
GCACGCA9850.0130.883682
CACGCAC9950.0130.300163
CGGTGCA950.0121.9826662
TCGCGCC203.8570256E-4108.81709145
ACGCACT12600.0103.483254
CGCACTT12700.0103.238815
AACGGCC501.9463187E-10101.56263145
AAACACG3450.0100.783695
GGCACCC750.096.72631145
GCGGTGC2500.092.7068251
GAAACAC3800.091.5009844
TGACGCC651.6370905E-1189.28582145
GGTGCAT1350.085.842283
ACGTGCA1900.083.863082
CGATAAA451.2287219E-680.605255145
ACACGTG1150.075.592397
ACAACGC2700.075.1280758
AACACGA3500.074.51256
Produced by FastQC (version 0.11.5)