FastQCFastQC Report
Tue 2 Jul 2019
HWC5JBCX2_l02_n02_SM2_9841_P2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l02_n02_SM2_9841_P2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences650782
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCTATAGTAGATCTATAGAAGTATAAATCTATAAATGAAACCCAATCA83771.2872206053640083No Hit
GATTACGTGATCGTTTGGGATAATGTAACTGCCGTTTTCCATCAGACCCA79891.2276000258150963No Hit
AGTAGAAACAACGCACTGATTACGTGATCGTTTGGGATAATGTAACTGCC66841.0270720456312559No Hit
CTGATTACGTGATCGTTTGGGATAATGTAACTGCCGTTTTCCATCAGACC56320.865420371184206No Hit
ATCTATAGTAGATCTATAGAAGTATAAATCTATAAATGAAACCCAATCAG55830.8578909680968435No Hit
GTATAAATCTATAAATGAAACCCAATCAGCATCGTTCTCAATACTGTTAC55460.8522055004594473No Hit
CTATAGAAGTATAAATCTATAAATGAAACCCAATCAGCATCGTTCTCAAT54780.8417565329096379No Hit
GGATAATGTAACTGCCGTTTTCCATCAGACCCAGTTCACTCACCGTCCCA42090.6467603590756966No Hit
ACAACGCACTGATTACGTGATCGTTTGGGATAATGTAACTGCCGTTTTCC40810.6270917142760556No Hit
CAACAGCACTGATTACGTGATCGTTTGGGATAATGTAACTGCCGTTTTCC40570.6234038433761229No Hit
ATAGTAGATCTATAGAAGTATAAATCTATAAATGAAACCCAATCAGCATC33290.5115384260781645No Hit
GTATTTGGCAAAGGCACATGTCTTGTGTTTTTAATCAGTCCAGAGTGGAG33070.5081578777532262No Hit
GTTTGGGATAATGTAACTGCCGTTTTCCATCAGACCCAGTTCACTCACCG32500.49939918436588593No Hit
TCTCAATACTGTTACTCTCTGGTCTGCATCAGGATCACAAGACAGAACTC31760.4880282490910935No Hit
GTCCAGAATGGAAAGGAGACAATCACTCCAATTCCAAACCCAAATGTGCA29990.46083020120408985No Hit
CTGTAGAACTGCTTTCACAGTCCAGAATGGAAAGGAGACAATCACTCCAA29290.4500739110792861No Hit
GCACTGATTACGTGATCGTTTGGGATAATGTAACTGCCGTTTTCCATCAG28880.44377379829190117No Hit
GTCCTATTTATGAGAAGAAAAACCAATCAGGGTATCAGAGAGCATTCTTC28460.43732002421701893No Hit
GATCTATAGAAGTATAAATCTATAAATGAAACCCAATCAGCATCGTTCTC27280.4191879922923498No Hit
CTATAAATGAAACCCAATCAGCATCGTTCTCAATACTGTTACTCTCTGGT27120.41672941169239475No Hit
TGGTAGTACCATACGAGGACAGCATCAGTGTGGGGTTCTTCTTTCAGACT25170.38676546063044154No Hit
GACAGGGACTGTCCACCACTGACATCCGGAGGATCAACAAACTCTATGGA23720.36448457394334816No Hit
GAATAAAACGTATTCATCAAGAGCTGTTTTACCTGTTTACATTACTAAAT23370.3591064288809463No Hit
ATATTGAGGTCATCTCAGTGATTTATTTTCCAGTATTGTGAATATTTTAG23000.35342096124355005No Hit
ATCTATAGAAGTATAAATCTATAAATGAAACCCAATCAGCATCGTTCTCA22570.34681352588117065No Hit
AAACAACGCACTGATTACGTGATCGTTTGGGATAATGTAACTGCCGTTTT22440.3448159291437071No Hit
GTCCACCACTGACATCCGGAGGATCAACAAACTCTATGGATGCTGTGAGT21420.32914247781899314No Hit
GTGTGGGGTTCTTCTTTCAGACTCGATCTGTCCTATTTATGAGAAGAAAA21100.3242253166190829No Hit
GGGTATCAGAGAGCATTCTTCCATCGTGATTCCTGTCATTTCAGTGTCCC21030.3231496876066025No Hit
CTCCATATGACTACACTTCTGTGATGCATTATGGGAGGTAAGAAAATGGG20640.3171568973942119No Hit
CTCTATGGATGCTGTGAGTATGGTGTTCAAACTCCATCAGATACATGGAA20430.31393001035677076No Hit
GTTCACTCACCGTCCCACAGTATTTGGCAAAGGCACATGTCTTGTGTTTT20410.3136226877817764No Hit
GTAGAAACACGTGCTAATCTGACAGATCTATAGTAGATCTATAGAAGTAT19580.3008688009195092No Hit
TACGAGGACAGCATCAGTGTGGGGTTCTTCTTTCAGACTCGATCTGTCCT18730.2878075914822475No Hit
GTAGAAACACGAGCTAATCTGACAGATCTATAGTAGATCTATAGAAGTAT18020.2768976400699466No Hit
GGGATATTGAGGTCATCTCAGTGATTTATTTTCCAGTATTGTGAATATTT17690.2718268175825392No Hit
ATATAAATCATCATTTAGCAAGTTGTCATGTGGTAGTACCATACGAGGAC17300.26583402737014855No Hit
ATGTAACTGCCGTTTTCCATCAGACCCAGTTCACTCACCGTCCCACAGTA17100.2627608016202046No Hit
CCATATGACTACACTTCTGTGATGCATTATGGGAGGTAAGAAAATGGGAC16430.2524654953578925No Hit
GATTACAAGAGCATCATAAACATCCAAACACTCTATGTGTACAGACAAGA16010.2460117212830103No Hit
CAACGCACTGATTACGTGATCGTTTGGGATAATGTAACTGCCGTTTTCCA15990.2457043987080159No Hit
GTACTTCCTTCATTGCTTCCTTAGCCTGAATTTCTTTTTGGTAACTGTGT15120.23233586669575987No Hit
CTATAGTAGATCTATAGAAGTATAAATCTATAAATGAAACCCAATCAGCA15060.2314138989707767No Hit
CTCCAATTCCAAACCCAAATGTGCAAATTGGACAGAGACAGGGACTGTCC14750.22665039905836365No Hit
GCATCAGTGTGGGGTTCTTCTTTCAGACTCGATCTGTCCTATTTATGAGA14510.22296252815843096No Hit
TTCCAGTATTGTGAATATTTTAGATACGATGCTCACAAGACAAATAAATA14470.22234788300844213No Hit
GAGTGTTCCTAGTTTTACTTGCATTGAATAATTTCCAGGATTCAGGTACA14390.22111859270846457No Hit
CTTTCCATGTATCTGATGGAGTTTGAACACCATACTCACAGCATCCATAG14060.21604777022105714No Hit
AATCAGCATCGTTCTCAATACTGTTACTCTCTGGTCTGCATCAGGATCAC13760.21143793159614124No Hit
CATGGAAAGAGGGCATCATTTTGTTTGTCTTTGGGGACACTGAAATGACA13710.21066962515865528No Hit
GCTTTCACAGTCCAGAATGGAAAGGAGACAATCACTCCAATTCCAAACCC13590.20882568970868892No Hit
TCCATAGAGTTTGTTGATCCTCCGGATGTCAGTGGTGGACAGTCCCTGTC13340.20498415752125904No Hit
GTTCTCAATACTGTTACTCTCTGGTCTGCATCAGGATCACAAGACAGAAC13310.20452317365876743No Hit
GTTTTACCTGTTTACATTACTAAATAAAAATATGTTTTATAAAACTTTTA13270.20390852850877866No Hit
GTTCAAATTTCCTTCTGTAGAACTGCTTTCACAGTCCAGAATGGAAAGGA13130.20175727048381792No Hit
CTGTTACTCTCTGGTCTGCATCAGGATCACAAGACAGAACTCCACTCTGG12880.19791573829638803No Hit
GTAGAAACAACGCACTGATTACGTGATCGTTTGGGATAATGTAACTGCCG12850.19745475443389646No Hit
GTATCAGAGAGCATTCTTCCATCGTGATTCCTGTCATTTCAGTGTCCCCA12800.19668644799641044No Hit
AATCTGACAGATCTATAGTAGATCTATAGAAGTATAAATCTATAAATGAA12410.19069365778401984No Hit
GTCCCACAGTATTTGGCAAAGGCACATGTCTTGTGTTTTTAATCAGTCCA12170.18700578688408714No Hit
GTCCCTGTCTCTGTCCAATTTGCACATTTGGGTTTGGAATTGGAGTGATT11970.18393256113414322No Hit
GTCATGTGGTAGTACCATACGAGGACAGCATCAGTGTGGGGTTCTTCTTT11960.18377889984664603No Hit
CTTCAGCACAGGTGGGGCCGTTGTAGGGCAGATCACACAGGCAGGTGAAG11870.18239594825917127No Hit
CAATACTGTTACTCTCTGGTCTGCATCAGGATCACAAGACAGAACTCCAC11430.17563485160929465No Hit
GTGCTAATCTGACAGATCTATAGTAGATCTATAGAAGTATAAATCTATAA11350.17440556130931711No Hit
GTGGTAGTACCATACGAGGACAGCATCAGTGTGGGGTTCTTCTTTCAGAC11090.17041036783439No Hit
ACGTGATCGTTTGGGATAATGTAACTGCCGTTTTCCATCAGACCCAGTTC10980.16872009367192084No Hit
GGGATAATGTAACTGCCGTTTTCCATCAGACCCAGTTCACTCACCGTCCC10980.16872009367192084No Hit
GTACCATACGAGGACAGCATCAGTGTGGGGTTCTTCTTTCAGACTCGATC10740.16503222277198817No Hit
TTGTAGGGCAGATCACACAGGCAGGTGAAGCTGGCCACACCATCCACACA10640.1634956098970162No Hit
GTTTACATTACTAAATAAAAATATGTTTTATAAAACTTTTACAAATCACT10620.1631882873220218No Hit
TAGTAGATCTATAGAAGTATAAATCTATAAATGAAACCCAATCAGCATCG10530.16180533573454703No Hit
TTCCTAGAGTGGCCACTAGGAGGAGTTCCAGCCTCAGTCAGTAATGATCT10510.16149801315955267No Hit
GTCCAGAGTGGAGTTCTGTCTTGTGATCCTGATGCAGACCAGAGAGTAAC10450.1605760454345695No Hit
TTATAAAACTTTTACAAATCACTGTTCAAATTTCCTTCTGTAGAACTGCT10420.1601150615720779No Hit
TTACGTGATCGTTTGGGATAATGTAACTGCCGTTTTCCATCAGACCCAGT10390.1596540777095863No Hit
CAACAGCAAAGAGGGATATTGAGGTCATCTCAGTGATTTATTTTCCAGTA10380.15950041642208912No Hit
CTCTAGGAATAGCCAGTTGGAGAGGTGGTGTTCTCAGGGTAGAGTAGCCT10370.1593467551345919No Hit
GTGAGTATGGTGTTCAAACTCCATCAGATACATGGAAAGAGGGCATCATT10090.15504423908467044No Hit
GTTACTCTCTGGTCTGCATCAGGATCACAAGACAGAACTCCACTCTGGAC10060.15458325522217886No Hit
GAGACAGGGACTGTCCACCACTGACATCCGGAGGATCAACAAACTCTATG10050.15442959393468167No Hit
AATAAATATAAATCATCATTTAGCAAGTTGTCATGTGGTAGTACCATACG10010.15381494878469287No Hit
GTAGTAACAAGAGCAACAACAGCAACAACATAACAATGTAAGAGACCTTG9610.14766849728480505No Hit
CCATACTCACAGCATCCATAGAGTTTGTTGATCCTCCGGATGTCAGTGGT9530.14643920698482749No Hit
GCATTATGGGAGGTAAGAAAATGGGACCCACTGAATAAAACGTATTCATC9470.1455172392598443No Hit
GCAAAGAGGGATATTGAGGTCATCTCAGTGATTTATTTTCCAGTATTGTG9450.14520991668484992No Hit
GTAGAAACAACAGCAAAGAGGGATATTGAGGTCATCTCAGTGATTTATTT9240.1419830296474088No Hit
GAGGGATATTGAGGTCATCTCAGTGATTTATTTTCCAGTATTGTGAATAT9230.1418293683599116No Hit
ATTGTGAATATTTTAGATACGATGCTCACAAGACAAATAAATATAAATCA9200.14136838449742No Hit
CACGTGCTAATCTGACAGATCTATAGTAGATCTATAGAAGTATAAATCTA9030.13875614260996771No Hit
CTATGGATGCTGTGAGTATGGTGTTCAAACTCCATCAGATACATGGAAAG8940.13737319102249296No Hit
GTATTGTGAATATTTTAGATACGATGCTCACAAGACAAATAAATATAAAT8660.13307067497257144No Hit
AGTAGAAACAACGCACCAACAACCTGAACACTCCATATGACTACACTTCT8590.1319950459600911No Hit
GAGTATGGTGTTCAAACTCCATCAGATACATGGAAAGAGGGCATCATTTT8420.12938280407263877No Hit
GTAGTAACAAGAGCATGTGAGATTATTTTTGAATTGACAATTATTTTAAA8290.12738520733517522No Hit
GTTGTCATGTGGTAGTACCATACGAGGACAGCATCAGTGTGGGGTTCTTC8250.12677056218518643No Hit
CTCTATGTGTACAGACAAGATCAACTCTAAATGACTGAGCATTCAGATGC8200.12600225574770046No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG8110.12461930416022571No Hit
GTGGTGGACAGTCCCTGTCTCTGTCCAATTTGCACATTTGGGTTTGGAAT8010.12308269128525375No Hit
ATTTATGAGAAGAAAAACCAATCAGGGTATCAGAGAGCATTCTTCCATCG7980.12262170742276216No Hit
GCATCGTTCTCAATACTGTTACTCTCTGGTCTGCATCAGGATCACAAGAC7930.12185340098527617No Hit
ACAACAGCACTGATTACGTGATCGTTTGGGATAATGTAACTGCCGTTTTC7790.11970214296031544No Hit
GGATTGAAAGGAAGAAAGAGTGACGGAGCAAAAAAGCGAAAGGGAGAGTG7760.11924115909782386No Hit
CTTTTCATACAGTATGGCTCAAACCCTTCAATTCCAGCAGATGGATTCAT7690.11816553008534349No Hit
CTAAATGACTGAGCATTCAGATGCTTGCTTTTCTGTGAACGGCCTCAGCT7620.1170899010728631No Hit
GATATACAGAAAGCACTAATTGGTGCCTCTATCTGCTTTTTAAAACCCAA7610.11693623978536591No Hit
AACAACGCACTGATTACGTGATCGTTTGGGATAATGTAACTGCCGTTTTC7600.11678257849786873No Hit
GTTCTACCCTCAGGCACATGGTCATGTTGAGATCAAGGCTACTCTACCCT7470.11478498176040518No Hit
TAATGTAACTGCCGTTTTCCATCAGACCCAGTTCACTCACCGTCCCACAG7450.11447765918541078No Hit
ATACTGTTACTCTCTGGTCTGCATCAGGATCACAAGACAGAACTCCACTC7370.11324836888543321No Hit
TCTCAGTGATTTATTTTCCAGTATTGTGAATATTTTAGATACGATGCTCA7340.11278738502294164No Hit
CTTCTGTGATGCATTATGGGAGGTAAGAAAATGGGACCCACTGAATAAAA7340.11278738502294164No Hit
GTTCAAACTCCATCAGATACATGGAAAGAGGGCATCATTTTGTTTGTCTT7210.11078978828547809No Hit
GTAGTACCATACGAGGACAGCATCAGTGTGGGGTTCTTCTTTCAGACTCG7050.10833120768552296No Hit
GTAGAACTGCTTTCACAGTCCAGAATGGAAAGGAGACAATCACTCCAATT6970.1071019173855454No Hit
ATCAGACCCAGTTCACTCACCGTCCCACAGTATTTGGCAAAGGCACATGT6940.10664093352305379No Hit
CTGATACCCTGATTGGTTTTTCTTCTCATAAATAGGACAGATCGAGTCTG6920.1063336109480594No Hit
ATTTTAGATACGATGCTCACAAGACAAATAAATATAAATCATCATTTAGC6900.10602628837306502No Hit
CAATCCTGTTTTACTCTTTGCGCAACTGAAGATCAAAACCTGTGTACTGT6880.10571896579807062No Hit
GGCACATGTCTTGTGTTTTTAATCAGTCCAGAGTGGAGTTCTGTCTTGTG6830.10495065936058465No Hit
AACCCAATCAGCATCGTTCTCAATACTGTTACTCTCTGGTCTGCATCAGG6810.10464333678559026No Hit
GGAGACAATCACTCCAATTCCAAACCCAAATGTGCAAATTGGACAGAGAC6790.10433601421059586No Hit
CCATCAGATACATGGAAAGAGGGCATCATTTTGTTTGTCTTTGGGGACAC6770.10402869163560148No Hit
ATAGAAGTATAAATCTATAAATGAAACCCAATCAGCATCGTTCTCAATAC6700.10295306262312111No Hit
GTAGAAACAACAGCACTGATTACGTGATCGTTTGGGATAATGTAACTGCC6630.10187743361064074No Hit
TGACTACACTTCTGTGATGCATTATGGGAGGTAAGAAAATGGGACCCACT6620.10172377232314356No Hit
GACCCAGTTCACTCACCGTCCCACAGTATTTGGCAAAGGCACATGTCTTG6560.10080180459816036No Hit
ACTCTGGACTGATTAAAAACACAAGACATGTGCCTTTGCCAAATACTGTG6540.10049448202316598No Hit
CCATCAGACCCAGTTCACTCACCGTCCCACAGTATTTGGCAAAGGCACAT6530.10034082073566877No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCCC202.1245578E-6145.0751145
AGCGTGA100.0071103186144.863313
CGTGAAG100.0071103186144.863315
GCGTGAA100.0071103186144.863314
TAACACG600.0132.791355
GGACGGG1150.0132.45987145
TAACCTT301.09786924E-7120.89593145
ACGATCC256.4465403E-6116.06008145
GGTGCCC256.493541E-6115.890643
CGGTGCC256.4960223E-6115.881732
TGACGCC650.0111.596245145
TGGAACC650.0100.43662145
ACTATAA750.096.716736145
CGACGAA1050.096.716736145
CCTTAGA451.0988515E-896.568122
GCCTTAG451.0988515E-896.568121
GCCGACC554.3763066E-879.13188145
ATATGCC300.001931464372.53756145
GAGAGCC300.001931464372.53756145
GGACGGC300.001931464372.53756145
Produced by FastQC (version 0.11.5)