FastQCFastQC Report
Thu 11 Jul 2019
HWC5JBCX2_l02_n02_SM2_9841_P2_DM2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l02_n02_SM2_9841_P2_DM2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences655235
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCTATAGTAGATCTATAGAAGTATAAATCTATAAATGAAACCCAATCA84081.2832037360641602No Hit
GATTACGTGATCGTTTGGGATAATGTAACTGCCGTTTTCCATCAGACCCA80211.224140957061207No Hit
AGTAGAAACAACGCACTGATTACGTGATCGTTTGGGATAATGTAACTGCC67161.024975772051249No Hit
CTGATTACGTGATCGTTTGGGATAATGTAACTGCCGTTTTCCATCAGACC56560.8632017520431601No Hit
ATCTATAGTAGATCTATAGAAGTATAAATCTATAAATGAAACCCAATCAG56050.855418285042771No Hit
GTATAAATCTATAAATGAAACCCAATCAGCATCGTTCTCAATACTGTTAC55670.8496188390424809No Hit
CTATAGAAGTATAAATCTATAAATGAAACCCAATCAGCATCGTTCTCAAT55080.8406144360420307No Hit
GGATAATGTAACTGCCGTTTTCCATCAGACCCAGTTCACTCACCGTCCCA42220.6443489740322175No Hit
ACAACGCACTGATTACGTGATCGTTTGGGATAATGTAACTGCCGTTTTCC41010.6258823170312942No Hit
CAACAGCACTGATTACGTGATCGTTTGGGATAATGTAACTGCCGTTTTCC40710.6213038070310652No Hit
ATAGTAGATCTATAGAAGTATAAATCTATAAATGAAACCCAATCAGCATC33430.51019863102551No Hit
GTATTTGGCAAAGGCACATGTCTTGTGTTTTTAATCAGTCCAGAGTGGAG33190.5065358230253267No Hit
GTTTGGGATAATGTAACTGCCGTTTTCCATCAGACCCAGTTCACTCACCG32600.4975314200248766No Hit
TCTCAATACTGTTACTCTCTGGTCTGCATCAGGATCACAAGACAGAACTC31860.4862377620243119No Hit
GTCCAGAATGGAAAGGAGACAATCACTCCAATTCCAAACCCAAATGTGCA30090.45922455302296117No Hit
CTGTAGAACTGCTTTCACAGTCCAGAATGGAAAGGAGACAATCACTCCAA29410.4488465970224423No Hit
GCACTGATTACGTGATCGTTTGGGATAATGTAACTGCCGTTTTCCATCAG29030.4430471510221524No Hit
GTCCTATTTATGAGAAGAAAAACCAATCAGGGTATCAGAGAGCATTCTTC28520.4352636840217632No Hit
GATCTATAGAAGTATAAATCTATAAATGAAACCCAATCAGCATCGTTCTC27370.41771272902088563No Hit
CTATAAATGAAACCCAATCAGCATCGTTCTCAATACTGTTACTCTCTGGT27210.4152708570207635No Hit
TGGTAGTACCATACGAGGACAGCATCAGTGTGGGGTTCTTCTTTCAGACT25260.38551054201927554No Hit
GACAGGGACTGTCCACCACTGACATCCGGAGGATCAACAAACTCTATGGA23860.3641441620182072No Hit
GAATAAAACGTATTCATCAAGAGCTGTTTTACCTGTTTACATTACTAAAT23510.35880256701794017No Hit
ATATTGAGGTCATCTCAGTGATTTATTTTCCAGTATTGTGAATATTTTAG23150.3533083550176654No Hit
ATCTATAGAAGTATAAATCTATAAATGAAACCCAATCAGCATCGTTCTCA22680.3461353560173068No Hit
AAACAACGCACTGATTACGTGATCGTTTGGGATAATGTAACTGCCGTTTT22570.3444565690172228No Hit
GTCCACCACTGACATCCGGAGGATCAACAAACTCTATGGATGCTGTGAGT21540.32873701801643684No Hit
GTGTGGGGTTCTTCTTTCAGACTCGATCTGTCCTATTTATGAGAAGAAAA21140.3226323380161316No Hit
GGGTATCAGAGAGCATTCTTCCATCGTGATTCCTGTCATTTCAGTGTCCC21100.32202187001610105No Hit
CTCCATATGACTACACTTCTGTGATGCATTATGGGAGGTAAGAAAATGGG20750.316680275015834No Hit
GTTCACTCACCGTCCCACAGTATTTGGCAAAGGCACATGTCTTGTGTTTT20500.31286485001564324No Hit
CTCTATGGATGCTGTGAGTATGGTGTTCAAACTCCATCAGATACATGGAA20500.31286485001564324No Hit
GTAGAAACACGTGCTAATCTGACAGATCTATAGTAGATCTATAGAAGTAT19700.3006554900150328No Hit
TACGAGGACAGCATCAGTGTGGGGTTCTTCTTTCAGACTCGATCTGTCCT18820.28722519401436125No Hit
GTAGAAACACGAGCTAATCTGACAGATCTATAGTAGATCTATAGAAGTAT18110.2763893870138195No Hit
GGGATATTGAGGTCATCTCAGTGATTTATTTTCCAGTATTGTGAATATTT17810.2718108770135905No Hit
ATATAAATCATCATTTAGCAAGTTGTCATGTGGTAGTACCATACGAGGAC17350.2647904950132395No Hit
ATGTAACTGCCGTTTTCCATCAGACCCAGTTCACTCACCGTCCCACAGTA17130.26143292101307164No Hit
CCATATGACTACACTTCTGTGATGCATTATGGGAGGTAAGAAAATGGGAC16470.251360199012568No Hit
CAACGCACTGATTACGTGATCGTTTGGGATAATGTAACTGCCGTTTTCCA16060.24510290201225515No Hit
GATTACAAGAGCATCATAAACATCCAAACACTCTATGTGTACAGACAAGA16060.24510290201225515No Hit
GTACTTCCTTCATTGCTTCCTTAGCCTGAATTTCTTTTTGGTAACTGTGT15160.23136737201156837No Hit
CTATAGTAGATCTATAGAAGTATAAATCTATAAATGAAACCCAATCAGCA15110.2306042870115302No Hit
CTCCAATTCCAAACCCAAATGTGCAAATTGGACAGAGACAGGGACTGTCC14780.22556792601127837No Hit
GCATCAGTGTGGGGTTCTTCTTTCAGACTCGATCTGTCCTATTTATGAGA14560.22221035201111053No Hit
TTCCAGTATTGTGAATATTTTAGATACGATGCTCACAAGACAAATAAATA14510.22144726701107237No Hit
GAGTGTTCCTAGTTTTACTTGCATTGAATAATTTCCAGGATTCAGGTACA14410.21992109701099607No Hit
CTTTCCATGTATCTGATGGAGTTTGAACACCATACTCACAGCATCCATAG14140.21580043801079002No Hit
AATCAGCATCGTTCTCAATACTGTTACTCTCTGGTCTGCATCAGGATCAC13820.21091669401054583No Hit
CATGGAAAGAGGGCATCATTTTGTTTGTCTTTGGGGACACTGAAATGACA13800.21061146001053058No Hit
GCTTTCACAGTCCAGAATGGAAAGGAGACAATCACTCCAATTCCAAACCC13620.20786435401039324No Hit
TCCATAGAGTTTGTTGATCCTCCGGATGTCAGTGGTGGACAGTCCCTGTC13390.2043541630102177No Hit
GTTCTCAATACTGTTACTCTCTGGTCTGCATCAGGATCACAAGACAGAAC13370.20404892901020244No Hit
GTTTTACCTGTTTACATTACTAAATAAAAATATGTTTTATAAAACTTTTA13310.20313322701015668No Hit
GTTCAAATTTCCTTCTGTAGAACTGCTTTCACAGTCCAGAATGGAAAGGA13200.20145444001007273No Hit
CTGTTACTCTCTGGTCTGCATCAGGATCACAAGACAGAACTCCACTCTGG12950.19763901500988193No Hit
GTAGAAACAACGCACTGATTACGTGATCGTTTGGGATAATGTAACTGCCG12940.19748639800987433No Hit
GTATCAGAGAGCATTCTTCCATCGTGATTCCTGTCATTTCAGTGTCCCCA12840.19596022800979798No Hit
AATCTGACAGATCTATAGTAGATCTATAGAAGTATAAATCTATAAATGAA12480.1904660160095233No Hit
GTCCCACAGTATTTGGCAAAGGCACATGTCTTGTGTTTTTAATCAGTCCA12210.18634535700931726No Hit
GTCCCTGTCTCTGTCCAATTTGCACATTTGGGTTTGGAATTGGAGTGATT12040.18375086800918755No Hit
GTCATGTGGTAGTACCATACGAGGACAGCATCAGTGTGGGGTTCTTCTTT11980.18283516600914174No Hit
CTTCAGCACAGGTGGGGCCGTTGTAGGGCAGATCACACAGGCAGGTGAAG11930.1820720810091036No Hit
CAATACTGTTACTCTCTGGTCTGCATCAGGATCACAAGACAGAACTCCAC11460.17489908200874496No Hit
GTGCTAATCTGACAGATCTATAGTAGATCTATAGAAGTATAAATCTATAA11430.17444123100872205No Hit
GTGGTAGTACCATACGAGGACAGCATCAGTGTGGGGTTCTTCTTTCAGAC11140.17001533800850077No Hit
ACGTGATCGTTTGGGATAATGTAACTGCCGTTTTCCATCAGACCCAGTTC11060.16879440200843973No Hit
GGGATAATGTAACTGCCGTTTTCCATCAGACCCAGTTCACTCACCGTCCC11050.16864178500843208No Hit
GTACCATACGAGGACAGCATCAGTGTGGGGTTCTTCTTTCAGACTCGATC10770.16436850900821842No Hit
TTGTAGGGCAGATCACACAGGCAGGTGAAGCTGGCCACACCATCCACACA10680.16299495600814976No Hit
GTTTACATTACTAAATAAAAATATGTTTTATAAAACTTTTACAAATCACT10660.16268972200813447No Hit
TAGTAGATCTATAGAAGTATAAATCTATAAATGAAACCCAATCAGCATCG10550.16101093500805053No Hit
TTCCTAGAGTGGCCACTAGGAGGAGTTCCAGCCTCAGTCAGTAATGATCT10530.16070570100803527No Hit
GTCCAGAGTGGAGTTCTGTCTTGTGATCCTGATGCAGACCAGAGAGTAAC10520.16055308400802765No Hit
TTATAAAACTTTTACAAATCACTGTTCAAATTTCCTTCTGTAGAACTGCT10430.15917953100795898No Hit
TTACGTGATCGTTTGGGATAATGTAACTGCCGTTTTCCATCAGACCCAGT10430.15917953100795898No Hit
CAACAGCAAAGAGGGATATTGAGGTCATCTCAGTGATTTATTTTCCAGTA10420.15902691400795133No Hit
CTCTAGGAATAGCCAGTTGGAGAGGTGGTGTTCTCAGGGTAGAGTAGCCT10400.15872168000793607No Hit
GTGAGTATGGTGTTCAAACTCCATCAGATACATGGAAAGAGGGCATCATT10150.15490625500774532No Hit
GTTACTCTCTGGTCTGCATCAGGATCACAAGACAGAACTCCACTCTGGAC10120.15444840400772242No Hit
AATAAATATAAATCATCATTTAGCAAGTTGTCATGTGGTAGTACCATACG10090.15399055300769954No Hit
GAGACAGGGACTGTCCACCACTGACATCCGGAGGATCAACAAACTCTATG10090.15399055300769954No Hit
GTAGTAACAAGAGCAACAACAGCAACAACATAACAATGTAAGAGACCTTG9670.14758063900737903No Hit
CCATACTCACAGCATCCATAGAGTTTGTTGATCCTCCGGATGTCAGTGGT9550.14574923500728748No Hit
GCAAAGAGGGATATTGAGGTCATCTCAGTGATTTATTTTCCAGTATTGTG9500.14498615000724932No Hit
GCATTATGGGAGGTAAGAAAATGGGACCCACTGAATAAAACGTATTCATC9480.14468091600723404No Hit
GAGGGATATTGAGGTCATCTCAGTGATTTATTTTCCAGTATTGTGAATAT9250.14117072500705855No Hit
GTAGAAACAACAGCAAAGAGGGATATTGAGGTCATCTCAGTGATTTATTT9250.14117072500705855No Hit
ATTGTGAATATTTTAGATACGATGCTCACAAGACAAATAAATATAAATCA9220.14071287400703564No Hit
CACGTGCTAATCTGACAGATCTATAGTAGATCTATAGAAGTATAAATCTA9070.13842361900692118No Hit
CTATGGATGCTGTGAGTATGGTGTTCAAACTCCATCAGATACATGGAAAG8980.1370500660068525No Hit
GTATTGTGAATATTTTAGATACGATGCTCACAAGACAAATAAATATAAAT8760.13369249200668462No Hit
AGTAGAAACAACGCACCAACAACCTGAACACTCCATATGACTACACTTCT8610.13140323700657014No Hit
GAGTATGGTGTTCAAACTCCATCAGATACATGGAAAGAGGGCATCATTTT8460.1291139820064557No Hit
GTAGTAACAAGAGCATGTGAGATTATTTTTGAATTGACAATTATTTTAAA8340.12728257800636414No Hit
GTTGTCATGTGGTAGTACCATACGAGGACAGCATCAGTGTGGGGTTCTTC8250.12590902500629544No Hit
CTCTATGTGTACAGACAAGATCAACTCTAAATGACTGAGCATTCAGATGC8220.12545117400627256No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG8180.12484070600624203No Hit
GTGGTGGACAGTCCCTGTCTCTGTCCAATTTGCACATTTGGGTTTGGAAT8010.12224621700611231No Hit
ATTTATGAGAAGAAAAACCAATCAGGGTATCAGAGAGCATTCTTCCATCG8000.12209360000610468No Hit
GCATCGTTCTCAATACTGTTACTCTCTGGTCTGCATCAGGATCACAAGAC7960.12148313200607416No Hit
ACAACAGCACTGATTACGTGATCGTTTGGGATAATGTAACTGCCGTTTTC7840.11965172800598259No Hit
GGATTGAAAGGAAGAAAGAGTGACGGAGCAAAAAAGCGAAAGGGAGAGTG7780.1187360260059368No Hit
CTTTTCATACAGTATGGCTCAAACCCTTCAATTCCAGCAGATGGATTCAT7710.11766770700588339No Hit
CTAAATGACTGAGCATTCAGATGCTTGCTTTTCTGTGAACGGCCTCAGCT7660.11690462200584523No Hit
GATATACAGAAAGCACTAATTGGTGCCTCTATCTGCTTTTTAAAACCCAA7650.11675200500583761No Hit
AACAACGCACTGATTACGTGATCGTTTGGGATAATGTAACTGCCGTTTTC7650.11675200500583761No Hit
GTTCTACCCTCAGGCACATGGTCATGTTGAGATCAAGGCTACTCTACCCT7510.11461536700573076No Hit
TAATGTAACTGCCGTTTTCCATCAGACCCAGTTCACTCACCGTCCCACAG7490.1143101330057155No Hit
ATACTGTTACTCTCTGGTCTGCATCAGGATCACAAGACAGAACTCCACTC7420.11324181400566209No Hit
TCTCAGTGATTTATTTTCCAGTATTGTGAATATTTTAGATACGATGCTCA7380.11263134600563156No Hit
CTTCTGTGATGCATTATGGGAGGTAAGAAAATGGGACCCACTGAATAAAA7370.11247872900562393No Hit
GTTCAAACTCCATCAGATACATGGAAAGAGGGCATCATTTTGTTTGTCTT7240.11049470800552474No Hit
GTAGTACCATACGAGGACAGCATCAGTGTGGGGTTCTTCTTTCAGACTCG7070.10790021900539501No Hit
GTAGAACTGCTTTCACAGTCCAGAATGGAAAGGAGACAATCACTCCAATT7000.1068319000053416No Hit
ATCAGACCCAGTTCACTCACCGTCCCACAGTATTTGGCAAAGGCACATGT6990.10667928300533397No Hit
CTGATACCCTGATTGGTTTTTCTTCTCATAAATAGGACAGATCGAGTCTG6940.10591619800529581No Hit
ATTTTAGATACGATGCTCACAAGACAAATAAATATAAATCATCATTTAGC6910.10545834700527291No Hit
CAATCCTGTTTTACTCTTTGCGCAACTGAAGATCAAAACCTGTGTACTGT6890.10515311300525766No Hit
GGCACATGTCTTGTGTTTTTAATCAGTCCAGAGTGGAGTTCTGTCTTGTG6850.10454264500522714No Hit
GGAGACAATCACTCCAATTCCAAACCCAAATGTGCAAATTGGACAGAGAC6830.10423741100521186No Hit
AACCCAATCAGCATCGTTCTCAATACTGTTACTCTCTGGTCTGCATCAGG6820.10408479400520423No Hit
CCATCAGATACATGGAAAGAGGGCATCATTTTGTTTGTCTTTGGGGACAC6810.10393217700519662No Hit
ATAGAAGTATAAATCTATAAATGAAACCCAATCAGCATCGTTCTCAATAC6730.10271124100513557No Hit
GTAGAAACAACAGCACTGATTACGTGATCGTTTGGGATAATGTAACTGCC6660.10164292200508214No Hit
TGACTACACTTCTGTGATGCATTATGGGAGGTAAGAAAATGGGACCCACT6630.10118507100505925No Hit
GACCCAGTTCACTCACCGTCCCACAGTATTTGGCAAAGGCACATGTCTTG6610.10087983700504399No Hit
CCATCAGACCCAGTTCACTCACCGTCCCACAGTATTTGGCAAAGGCACAT6580.1004219860050211No Hit
ACTCTGGACTGATTAAAAACACAAGACATGTGCCTTTGCCAAATACTGTG6570.10026936900501347No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCAGCC100.007072678145.12077145
GATCCCC100.007072678145.12077145
AGACGCC100.007072678145.12077145
AAGTCGG100.007072678145.12077145
CGATACC151.233223E-4144.899267
GGACGGG1250.0139.31593145
ACTATAA650.0133.95764145
GAGAGCC458.367351E-11112.87171145
GCCGACC458.367351E-11112.87171145
TGACGCC501.9463187E-10101.58455145
TAACACG501.9645086E-10101.421745
TATCCCC950.099.29316145
GTGGTCC259.3542953E-487.07247145
CGATATT502.2544555E-887.07247145
GGGGCGG259.3542953E-487.07247145
ATATGCC353.422225E-582.926155145
GCACGCA2550.082.381122
TCTTCCC451.2263208E-680.62265145
CGACGAA950.076.37936145
TAACCTT502.293973E-672.560394145
Produced by FastQC (version 0.11.5)