FastQCFastQC Report
Thu 11 Jul 2019
HWC5JBCX2_l02_n02_SM2_9839_P2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l02_n02_SM2_9839_P2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences272095
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATATTACTGCTGCATCTTTAAATGACGATGGATTGGATAATCATACTA7150.2627758687223212No Hit
ATTATTATGTGGTCATTCAATACCTCCATGTTGTCAGAGAGCACTTCCTT6300.23153677943365367No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC6170.2267590363659751No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA5110.18780205442951908No Hit
AGCACGCACTTTCTTAAAATGTCACTGTTTGGAGACACAATTGCCTACTT5070.18633197963946416No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC4950.18192175526929932No Hit
TCATAATCCTCTGCTGTGTCCCTCCCAAAGAAGAAATTGGGGATGGTCTG4630.17016115694885978No Hit
ATACTATACTGCTTTACTACTCAACTGCTGCCTCCAGTTTGGCTGTAACA4520.16611845127620867No Hit
GTATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTCAT4380.16097318951101638No Hit
GTATAGTATGATTATCCAATCCATCGTCATTTAAAGATGCAGCAGTAATA4150.15252025946820044No Hit
GGTCATATGCATTCAATCTATGCAGAGTTGATAAGGACTTGTATCCATTG4060.14921259119057684No Hit
GTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCGAT3890.14296477333284333No Hit
CTGTAGAACATGCTTGTAAATTCAAACATTCCAGTCTCATTACAGTAACT3790.13928958635770597No Hit
ATCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG3630.13340928719748618No Hit
CAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGCAAACAAG3610.1326742498024587No Hit
CCATTAGAAAGATATAATGAAGAAACAAGGGCAAAATTGAAGAAGCTAAA3570.13120417501240378No Hit
GTATTTACACAGTCTCTCCAAAGCTTCCATAAGAAATTTTCGTTAGCTTT3360.1234862823646153No Hit
CAATTATTATGTGGTCATTCAATACCTCCATGTTGTCAGAGAGCACTTCC3240.11907605799445047No Hit
GTTTAATATGCTATCTACCGTGTTGGGAGTAGCTGCACTAGGTATCAAGA3150.11576838971682683No Hit
CTACTATACTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCGAT3120.11466583362428563No Hit
GTAATATTCAGTGAATCAAAGGTGGGGAGAGAAAATTCTCCTGCATCAAA3070.11282824013671695No Hit
GTGTGAATGTGATGCTTGTTTCTCGCATAAAGCACAGAGCGTTCCTAGTT3060.11246072143920323No Hit
CTCAAGGACAATACATTACGCATATCCCTTTTTATTGTCAAACGGAACTT3050.11209320274168948No Hit
GGAAAATACAGGGCTTAACTTTCCTTATAGACAAATGGAGCAAGAAACAT3030.11135816534666203No Hit
GTTCTACAGAGATGGATTTGTATCTAATTTTGCAATGGAACTCCCTTCGT2980.10952057185909334No Hit
TCTATATGCTTTTTAAAACCCAAAGACCAGGAAAGAAAAAGAAGATTCAT2870.10547786618644224No Hit
GAATAAACACCCACATTCCAAACGTATTGAAACAATCACAGTCATAGTGT2850.10474282879141476No Hit
ATATTACTCTTAGTGCTTTTCCAAAGGATCCTTTTACTAGTTTTCCTTCT2790.10253771660633235No Hit
GAATACAACAATAACCTCTTTTAGGTTGAATGATTTAAATGGAGCCGACA2780.10217019790881861No Hit
GTAAGAAATACAGTAAAATTAGATTTAATGCAATTCTTCTATCTCCAAAA2780.10217019790881861No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAAGCGG151.2344675E-4144.839371
CACGCAC600.0120.699473
GCACGCA600.0120.699472
AGCACGC600.0120.699471
GAAACAC1100.0111.9213264
AAACACG1250.098.490775
ACGCACT750.096.559584
TAGAAAC1500.091.73162
GTAACAA2600.089.131914
ACGTGCA259.420741E-486.903622
ACGAGCC608.3309715E-1084.489639
ACACGAG1400.082.765357
AACACGA1400.082.765356
GCGGAGC451.2386045E-680.466311
GAGAATA554.4161425E-879.003299
CACGAGC1500.077.2476658
GGAGCAT300.001942746172.4196853
CGCACTT1000.072.4196855
CGGAGCA300.001942746172.4196852
TAGTAAC3400.070.289692
Produced by FastQC (version 0.11.5)