FastQCFastQC Report
Thu 11 Jul 2019
HWC5JBCX2_l02_n02_SM2_9839_P2_DM2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l02_n02_SM2_9839_P2_DM2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1946361
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATATTACTGCTGCATCTTTAAATGACGATGGATTGGATAATCATACTA50740.26069161887234693No Hit
ATTATTATGTGGTCATTCAATACCTCCATGTTGTCAGAGAGCACTTCCTT43810.22508671310204018No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC42720.21948651868795152No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA38310.1968288513795745No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGACAACAAGC36990.19004696456618272No Hit
AGCACGCACTTTCTTAAAATGTCACTGTTTGGAGACACAATTGCCTACTT35040.18002826813730854No Hit
TCATAATCCTCTGCTGTGTCCCTCCCAAAGAAGAAATTGGGGATGGTCTG33410.1716536654813778No Hit
GTATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTCAT32330.16610484899769365No Hit
ATACTATACTGCTTTACTACTCAACTGCTGCCTCCAGTTTGGCTGTAACA32250.16569382555445777No Hit
GTATGAACAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACCGAT29050.1492528878250232No Hit
GTATAGTATGATTATCCAATCCATCGTCATTTAAAGATGCAGCAGTAATA28470.14627296786156319No Hit
CAAGAGCATTTTTCAATAACGTTTCTTTGTAATGACAACAAGCAAACAAG27700.142316867220418No Hit
CTGTAGAACATGCTTGTAAATTCAAACATTCCAGTCTCATTACAGTAACT27270.14010761621302523No Hit
CCATTAGAAAGATATAATGAAGAAACAAGGGCAAAATTGAAGAAGCTAAA26350.1353808466158128No Hit
GGTCATATGCATTCAATCTATGCAGAGTTGATAAGGACTTGTATCCATTG25010.12849620394161207No Hit
ATCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG24060.12361530055318617No Hit
CTACTATACTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCGAT23340.11991608956406341No Hit
GTATTTACACAGTCTCTCCAAAGCTTCCATAAGAAATTTTCGTTAGCTTT22750.1168847916701989No Hit
GTAATATTCAGTGAATCAAAGGTGGGGAGAGAAAATTCTCCTGCATCAAA22490.11554896547968233No Hit
TCTATATGCTTTTTAAAACCCAAAGACCAGGAAAGAAAAAGAAGATTCAT22250.11431589514997476No Hit
GTTTAATATGCTATCTACCGTGTTGGGAGTAGCTGCACTAGGTATCAAGA22140.11375073791552544No Hit
CAATTATTATGTGGTCATTCAATACCTCCATGTTGTCAGAGAGCACTTCC21900.11251766758581783No Hit
GTGTGAATGTGATGCTTGTTTCTCGCATAAAGCACAGAGCGTTCCTAGTT21830.11215802207298646No Hit
CTCAAGGACAATACATTACGCATATCCCTTTTTATTGTCAAACGGAACTT20680.10624956007647092No Hit
TTCTTATAGGGCTGAAATAGATGGTTTTATCATCTCTTACCATCTTGTAA20620.10594129249404402No Hit
GAATAAACACCCACATTCCAAACGTATTGAAACAATCACAGTCATAGTGT20340.10450271044271849No Hit
GGAAAATACAGGGCTTAACTTTCCTTATAGACAAATGGAGCAAGAAACAT20340.10450271044271849No Hit
GTTCTACAGAGATGGATTTGTATCTAATTTTGCAATGGAACTCCCTTCGT20180.10368066355624676No Hit
ATATTACTCTTAGTGCTTTTCCAAAGGATCCTTTTACTAGTTTTCCTTCT19640.10090625531440468No Hit
GAATACAACAATAACCTCTTTTAGGTTGAATGATTTAAATGGAGCCGACA19500.10018696428874191No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCGAA253.6927304E-8145.13133145
CGCACCC400.0145.13133145
TATCGGG151.2257542E-4145.13133145
TGACGCC850.0110.98277145
AGCACGC3800.0108.633191
CACGCAC3850.0107.225123
GTAACAA17700.0106.385514
GCACGCA3900.0105.850432
GAGAATA3000.0103.834399
ACCCGCC352.7478745E-7103.66523145
CCACTAC352.7800706E-7103.462834
CGGTGCA352.7800706E-7103.462832
ACGCACT5000.088.3572544
CGCACTT4950.087.7866445
AGACGGG259.3543885E-487.0788145
GAAACAC4100.084.7890554
ATAAGCC702.910383E-1182.93218145
AAACACG4200.081.045895
ACCACTA451.2410455E-680.471093
TGAGAAT3800.080.064028
Produced by FastQC (version 0.11.5)