FastQCFastQC Report
Thu 11 Jul 2019
HWC5JBCX2_l02_n02_SM2_9831_P2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l02_n02_SM2_9831_P2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences278662
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG10580.37967142990432856No Hit
CTACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAAT8260.2964164471653831No Hit
CATTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAGCTCTGCTT8080.2899570088494305No Hit
GATATACAGAAAGCACTAATTGGTGCCTCTATCTGCTTTTTAAAACCCAA7430.266631259375157No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA7380.26483697095405906No Hit
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG6470.23218092169007615No Hit
CTATAGAACATGCTTGTAAATTCAAACATTCCAGTTTCGTTACAGTAACT6460.2318220640058566No Hit
GCTTTAGCACTTCCATTACATCCTTTGCAATTCCTTCCCCATTTTTCTGA6170.2214151911634884No Hit
ATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAG6140.2203386181108296No Hit
ATATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTCAT5860.210290602952681No Hit
GTCCTATTGTCCAAGCCATGAAATGGAGAGCTGATAAAATAAAAGAACAA5730.2056254530578263No Hit
ACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGC5540.19880715705765406No Hit
GTAAGAAATACAGTAAAAATTGAATTTAATGCGATTCTTCTACCTCCAAA5450.19557743789967774No Hit
GTATGAAAAGTTCCTCAGAGAGCAACTGTCCGAAATACAGTTGGATTGAT5360.19234771874170142No Hit
GTTCATAGCTGAGACCATCTGCATTTCCCGTCTCACTCCTGGCACTGAGG5340.19163000337326222No Hit
CATTCACACAGAGCTCATAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGT5290.18983571495216428No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT5010.17978769979401568No Hit
GGTCAAGACCGTCTAAACAGACTAAAGAGGAAATTAGAGTCAAGAATAAA4800.17225168842540425No Hit
GCTATGAACACAGCAAAAACAATGAATGGAATGGGGAAAGGAGAAGACGT4270.15323223116176585No Hit
ATTACATCCTTTGCAATTCCTTCCCCATTTTTCTGACTTGCCCCAAGGGA4250.15251451579332667No Hit
GTGTGAATGTGATGCTTGTTTTTCGCACAAAGCACAGAGTGTTCCTAGTT4150.14892593895113077No Hit
GCTATGAGCTCTGTGTGAATGTGATGCTTGTTTTTCGCACAAAGCACAGA4110.1474905082142524No Hit
GCTCATAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGTGAGACGGGAAAT4100.1471316505300328No Hit
CTCTTAAGGTCTGCCGCCACTGCCACTCCATGTGCTCCGTGAGATGTGTA4080.14641393516159362No Hit
GCCTTGGAATGGATAAAAAACAAAAGATGCTTAACTGATATACAGAAAGC4030.14461964674049566No Hit
GTTCTATAGAGATGGATTTGTATCTAACTTTGCAATGGAAATTCCTTCAT3990.1431842160036173No Hit
GTCATGTACCTGAATCCTGGAAATTATTCAATGCAAGTAAAACTAGGAAC3910.14031335452986055No Hit
GTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAGTAAAA3810.1367247776876646No Hit
CTCTTGAAGAGAGAAAAGCAATTGGAGTAAAAATGATGAAAGTACTTTTA3750.13457163158234708No Hit
CAATATGACCACAACACAAATTGAGGTGGGTCCGGGAGCAACCAATGCCA3690.13241848547702953No Hit
TGACTACCCTGGTCAAGACCGTCTAAACAGACTAAAGAGGAAATTAGAGT3670.13170077010859033No Hit
CTATTGTCCAAGCCATGAAATGGAGAGCTGATAAAATAAAAGAACAAATT3650.13098305474015115No Hit
GGTCATATGCATTCAATCTATGCAGAGTTGATAAGGACTTGTATCCATTG3640.13062419705593156No Hit
GTATGGCTCAAACCCTTCAATTCCAGCAGATGGATTCATAAATAAAAGTA3620.1299064816874924No Hit
CCATAGAGGCTCTTCATTTGGGTTTTATTATCCGAATGGAACCCCCAAAC3610.1295476240032728No Hit
GAGCCATACTGTATGAAAAGTTCCTCAGAGAGCAACTGTCCGAAATACAG3460.1241647587399789No Hit
ACTCTGTGCTTTGTGCGAAAAACAAGCATCACATTCACACAGAGCTCATA3390.12165275495044174No Hit
GTCCAAGCCATGAAATGGAGAGCTGATAAAATAAAAGAACAAATTGAAAG3380.12129389726622215No Hit
GTATTGTCCCTGAGAGTATTAGTAAACGGAACATTCCTCAAACACCCCAA3290.11806417810824583No Hit
TAATTGTACTACTCATGGTAGTAACATCCAATGCAGATCGAATCTGCACT3290.11806417810824583No Hit
CTTTTCATACAGTATGGCTCAAACCCTTCAATTCCAGCAGATGGATTCAT3280.11770532042402625No Hit
CTCCAATGTTGCTTTGCAGTTCTTCTGCCAGTTTTTGGACGTCTTCTCCT3200.1148344589502695No Hit
GTTCAAACATTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAGC3200.1148344589502695No Hit
TTCTAATGGTATGCTGAACAGATCAGGACAAGGTATTTGAGCCTTTAGTC3170.11375788589761072No Hit
ACATTACGCATATCCCTTCTTATTGTCAAACGGAACTTCCCTTCTTTCTG3140.11268131284495195No Hit
GTACAAATGTATGGTACTTCCACTGTTTGTGGGTTCGTTGCATTTTTGTA3130.11232245516073236No Hit
CAATACTCCAATGTTGCTTTGCAGTTCTTCTGCCAGTTTTTGGACGTCTT3110.11160473979229318No Hit
CTGTAAGATCATCAGTAGCAACAAGTTTAGCAACAAGCCTTCCACTCTGG3060.1098104513711952No Hit
TTTTATTACTTTGCTCCTGCCACTCGCGCACCACACCTTTTGAGGCAACA3060.1098104513711952No Hit
CCATTACATCCTTTGCAATTCCTTCCCCATTTTTCTGACTTGCCCCAAGG3050.10945159368697562No Hit
GGTTCAAACATTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAG3030.10873387831853644No Hit
CAACAAAAGTGAGCCTGAAAGTAAAAGGATGTCTCTTGAAGAGAGAAAAG3020.10837502063431684No Hit
GTATAGATCTGTTCCTTTTGGTTCTCCAGCTATGAGTTCCAGACACTGCA3020.10837502063431684No Hit
CCTTAAGAGTACACAAGAAGCTATAAATAAGATAACCAAAAATCTCAATT2950.1058630168447797No Hit
GTAATAAAAGGGTCATTGCCTTTAATTGGTGAAGCAGATTGCCTTCATGA2920.10478644379212092No Hit
GCTAATGGAGTGACCACACATTATGTTTCTCAGATTGGCGACTTCCCAGA2910.10442758610790133No Hit
GAGTGTTCCTAGTTTTACTTGCATTGAATAATTTCCAGGATTCAGGTACA2900.10406872842368174No Hit
CTGATATACAGAAAGCACTAATTGGTGCCTCTATCTGCTTTTTAAAACCC2880.10335101305524255No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTGCA151.235937E-4144.797062
AGCACGC458.54925E-11112.6199341
GCACGCA501.9645086E-10101.357952
CACGCAC554.2018655E-1092.143583
ACAACGC502.2790118E-886.9094248
CAACGCA554.4145054E-879.008579
CACGTGC554.087766E-665.816841
TGTGATC451.198514E-464.377358
GTGATCT502.0180792E-457.9396179
AAACAAC753.7830796E-757.929226
GACGACC400.006032570354.406284145
ACGAGCC400.00607128954.318399
ACACTAG400.006079918254.2988971
GTAACAA2950.053.9921234
ACGCACT953.1632226E-853.3462834
GAAACAC701.70689E-551.7132344
CGCACTT1004.7388312E-850.6789745
GTATGCT1800.048.2830169
AGCACTG450.00968533648.2656865
GGTATGC1850.046.9780658
Produced by FastQC (version 0.11.5)