FastQCFastQC Report
Thu 11 Jul 2019
HWC5JBCX2_l02_n02_SM2_9825_P2_DM2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l02_n02_SM2_9825_P2_DM2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2193303
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT111850.5099614599533215No Hit
ATACTATACTGCTCTATTACTCAACTGCTGCTTCTAGTTTGGCTGTAACA87530.3990784674985627No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC85400.38936708699162864No Hit
GTAAGAAATACAGTAAAAATTGAATTTAATGCGATTCTTCTACCTCCAAA81560.3718592460777193No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC66590.30360602251490104No Hit
TTCTAATACTTTACTTCCCTCCTTTTCAAAACCCAACCATTCATTGAACC59720.2722834008798602No Hit
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG59330.2705052607870413No Hit
CATTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAGCTCTGCTT55140.25140165312316626No Hit
CTACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAAT53480.24383315939475758No Hit
GAATAAAGGGGCCAAATAAAGAGACAATAAACAGAGAGGTATCAATTTTG50750.2313861787450252No Hit
ATATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTCAT50330.22947125864506637No Hit
CAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGCAAACAAG50200.2288785452807934No Hit
GATATACAGAAAGCACTAATTGGTGCCTCTATCTGCTTTTTAAAACCCAA48760.2223131049380774No Hit
ACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGC47920.21848326473815974No Hit
CAATAATACAAAAGGCCAAAAACACAATGGCAGAATTTAGTGAAGATCCT46250.21086917767403776No Hit
GCTTTAGCACTTCCATTACATCCTTTGCAATTCCTTCCCCATTTTTCTGA44320.2020696638813698No Hit
ATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAG43950.20038271045997746No Hit
GTATTACCCAAGGGTTGTTACTGATACATTCTTCTATTCCAGAATACATT39800.18146147613895572No Hit
CTATAGAACATGCTTGTAAATTCAAACATTCCAGTTTCGTTACAGTAACT38590.17594468251764575No Hit
ATCTAGAGGTTTGCTATGTAATAAGTGACATGAATTTTCTTGACGAAGAA35860.16349770186791338No Hit
GTCCTATTGTCCAAGCCATGAAATGGAGAGCTGATAAAATAAAAGAACAA35090.15998701501798884No Hit
GTTCATAGCTGAGACCATCTGCATTTCCCGTCTCACTCCTGGCACTGAGG35060.15985023501084894No Hit
AGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACATTGATTACAAT34920.15921192831086264No Hit
GTGTGAATGTGATGCTTGTTTTTCGCACAAAGCACAGAGTGTTCCTAGTT34780.15857362161087638No Hit
GTAATAAGTGACATGAATTTTCTTGACGAAGAAGGAAAAGCATATACAGC33330.15196258793244707No Hit
CATTCACACAGAGCTCATAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGT33000.150458007853908No Hit
GTCATAATGGATACTTTTATTACAAGAAACTTCCAGACTACAATAATACA32480.14808715439681613No Hit
AGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGCAAACAAGCA32390.1476768143753964No Hit
CTTTTATTACAAGAAACTTCCAGACTACAATAATACAAAAGGCCAAAAAC32340.14744884769682987No Hit
ATTACATCCTTTGCAATTCCTTCCCCATTTTTCTGACTTGCCCCAAGGGA31630.14421172086118517No Hit
AGCAGGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT29020.13231186024001246No Hit
ATAATAAACAGTGAAGATGAGCATCTATTGGCACTTGAGAGAAAACTAAA28830.13144558686145966No Hit
CTGTATTACCCAAGGGTTGTTACTGATACATTCTTCTATTCCAGAATACA28130.12825405336152826No Hit
GTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAGTAAAA26850.12241810639022516No Hit
GCTATGAGCTCTGTGTGAATGTGATGCTTGTTTTTCGCACAAAGCACAGA26820.12228132638308524No Hit
GCTGTAGACATAGGAAACGGATGCTTCGAAACCAAACACAAATGCAACCA26720.12182539302595219No Hit
GCCTTGGAATGGATAAAAAACAAAAGATGCTTAACTGATATACAGAAAGC26620.12136945966881912No Hit
ATATTACTCTTAGTGCTTTTCCAAAGGATCCTTTTACTAGTTTCCCTTCT26410.12041199961883972No Hit
GCTATGAACACAGCAAAAACAATGAATGGAATGGGGAAAGGAGAAGACGT26310.11995606626170666No Hit
GTCATGTACCTGAATCCTGGAAATTATTCAATGCAAGTAAAACTAGGAAC26220.11954572624028689No Hit
CCTTTAATGCAGGAGAATTTTCTCTCCCCACTTTTGACTCATTGAACATT25620.11681012609748857No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAATCATTTGTTTATCACATGTAT25470.116126226061789No Hit
ATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATGTT24100.10987993906906617No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA24090.10983434573335285No Hit
CTCCAATGTTGCTTTGCAGTTCTTCTGCCAGTTTTTGGACGTCTTCTCCT23920.10905925902622667No Hit
CTATAAGCTCACAAATAGAACTTGCAGTCTTGCTTTCCAACGAAGGAATA23730.10819298564767384No Hit
GTACTAAATTGAGTACTATGTCTTTTATTCATCCATTATTTCATCTACTG23490.10709874559055452No Hit
CTATTGTCCAAGCCATGAAATGGAGAGCTGATAAAATAAAAGAACAAATT23350.10646043889056825No Hit
CCATACTATACTGCTCTATTACTCAACTGCTGCTTCTAGTTTGGCTGTAA23060.10513823215488238No Hit
ATACTGCTCTATTACTCAACTGCTGCTTCTAGTTTGGCTGTAACATTAAT23060.10513823215488238No Hit
GTTCAAACATTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAGC22820.10404399209776305No Hit
GTTCTATAGAGATGGATTTGTATCTAACTTTGCAATGGAAATTCCTTCAT22720.10358805874062998No Hit
GTCCAAGCCATGAAATGGAGAGCTGATAAAATAAAAGAACAAATTGAAAG22610.10308653204778365No Hit
CCATATAAACAATAAAAATAGCTAGCATTAATGTTACAGCCAAACTAGAA22570.10290415870493042No Hit
CCTCAGAGCTGACACTATAAGCTCACAAATAGAACTTGCAGTCTTGCTTT22380.10203788532637761No Hit
AGCATATACAGCATTAGAAGGACAAGGGAAAGAACAAAATTTGAGACCAC22370.10199229199066431No Hit
GTCTTTTATTCATCCATTATTTCATCTACTGATTCTAATACTTTACTTCC22240.10139957862639133No Hit
ATTGAGTACTATGTCTTTTATTCATCCATTATTTCATCTACTGATTCTAA22210.10126279861925143No Hit
CTTCCATTACATCCTTTGCAATTCCTTCCCCATTTTTCTGACTTGCCCCA22200.10121720528353813No Hit
GCTCATAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGTGAGACGGGAAAT22170.1010804252763982No Hit
GGTTCAAACATTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAG22150.1009892386049716No Hit
ATTCAACATCTGTGTCCATCTAGAGGTTTGCTATGTAATAAGTGACATGA21980.1002141518978454No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCCC100.0070800846145.07771145
TCGCGCC100.0070800846145.07771145
CAGGCGG100.0071100877144.872654
CGGTGCA600.0132.79692
GCACGCA13750.0132.226212
CACGCAC13900.0131.844543
AGCACGC13750.0131.69941
CCCGTAC501.9645086E-10101.410853
AAGCGGT900.096.5795752
CGTACAG554.1836756E-1092.195895
CGGTGCC800.090.543342
ACGCACT20850.090.328274
GAAACAC8350.090.220094
CGCACTT21000.090.032115
GGTGCAT900.088.5332953
TAGTAAC38200.087.2252
AACGGCC259.368289E-487.04662145
GCTAGCC502.2615495E-887.04662145
CGCACCC502.2615495E-887.04662145
AAACACG8850.085.126795
Produced by FastQC (version 0.11.5)