FastQCFastQC Report
Tue 2 Jul 2019
HWC5JBCX2_l02_n02_SM2_9823_P2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l02_n02_SM2_9823_P2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1316024
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG39810.30250208202889917No Hit
GTCCTTGAGAGTGTTGGTAAACGGAACATTCCTCAAACACCCCAATGGAT22840.17355306590153372No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA21790.1655744880032583No Hit
CTCAAGGACAATACATTACGCATATCCCTTTTTATTGTCAAACGGAACTT20890.15873570694759367No Hit
GTATGAAAAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACTGAT19080.14498215838009032No Hit
GTGTAATACCACTGACAACAACACCCACCAAATCTCATTTTGCAAATCTC18810.14293052406339093No Hit
GTAATATTCAGTGAATCAAAGGTGGGGAGAGAAAATTCTCCTGCATCAAA17800.13525589198981172No Hit
ATAATAAACAGTGAAGATGAACATCTCTTGGCACTTGAAAGAAAGCTGAA16430.12484574749396667No Hit
GTTTATTATTCCTTCATTGGAAAGCAGGACTGCGAGTTCTATTTGTGAGC16070.12211023507170081No Hit
GGTCATATGCATTCAATCTATGCAGAGTTGATAAGGACTTGTATCCATTG14990.11390369780490325No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGGCAACAAGC14400.10942049689063423No Hit
GTGTAGTAAGGCTTGCTTTTGTTTAATCCACCGTATTTTTCGTGGAGGCA14380.1092685239782861No Hit
TCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACACT13890.10554518762575758No Hit
TAATTGTACTACTCATGGTAGTAACATCCAATGCAGATCGAATCTGCACT13690.10402545850227654No Hit
CTTATAGACAGATGGAGCAAGAAACATTGTCTCTGGAGACCATATAAACA13570.10311362102818794No Hit
TTACTACACAGGGGAACATGCAAAGGCCATAGGAAATTGCCCAATATGGG13560.10303763457201388No Hit
TCTCAGAGCTGATACAATAAGCTCACAAATAGAACTCGCAGTCCTGCTTT13360.10151790544853286No Hit
CTGTAGAACATGCTTGTAAATTCAAACATTCCAGTCTCATTACAGTAACT13340.10136593253618476No Hit
ACATTACGCATATCCCTTTTTATTGTCAAACGGAACTTCCCTTCTTTCTG13210.10037810860592207No Hit
GTATGTTATCATCAATTCTTTTACTTAAAAGATAAAGTTCTTCCGTGACC13180.10015014923739993No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAATT256.440927E-6116.08937145
AGCACGC2500.0115.877661
GCACGCA2550.0113.609862
CGCACCC458.367351E-11112.86467145
CACGCAC2600.0111.4250643
TCCCGGG203.8545023E-4108.833786145
CGGTGCA800.099.586152
GTAACAA11650.088.900944
TCTGACC259.358732E-487.06703145
TGACGCC259.358732E-487.06703145
ACGTGCA1200.084.497342
CGCACTT3350.084.317185
ATAAGCC801.8189894E-1281.62534145
GAGCAGA1250.075.320481
ACGCACT3900.074.283384
GCCATCC406.638585E-572.55586145
CGACGAA903.6379788E-1272.555855145
GCTGCCC607.9908204E-872.555855145
AATGGCC1000.072.555855145
AACGGCC300.001929972872.555855145
Produced by FastQC (version 0.11.5)