FastQCFastQC Report
Thu 11 Jul 2019
HWC5JBCX2_l02_n02_SM2_9823_P2_DM2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l02_n02_SM2_9823_P2_DM2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1325006
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG39950.301508068642708No Hit
GTCCTTGAGAGTGTTGGTAAACGGAACATTCCTCAAACACCCCAATGGAT22950.17320676283730038No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA21860.16498038499448306No Hit
CTCAAGGACAATACATTACGCATATCCCTTTTTATTGTCAAACGGAACTT20950.15811249156607593No Hit
GTATGAAAAGTTCCTCAAATAGCAACTGTACGAAATACAATTGGACTGAT19210.14498047556011068No Hit
GTGTAATACCACTGACAACAACACCCACCAAATCTCATTTTGCAAATCTC18890.14256539215671477No Hit
GTAATATTCAGTGAATCAAAGGTGGGGAGAGAAAATTCTCCTGCATCAAA17870.1348673138083903No Hit
ATAATAAACAGTGAAGATGAACATCTCTTGGCACTTGAAAGAAAGCTGAA16560.1249805661257383No Hit
GTTTATTATTCCTTCATTGGAAAGCAGGACTGCGAGTTCTATTTGTGAGC16120.12165982644606892No Hit
GGTCATATGCATTCAATCTATGCAGAGTTGATAAGGACTTGTATCCATTG15040.11350891995960773No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGGCAACAAGC14470.10920705264730876No Hit
GTGTAGTAAGGCTTGCTTTTGTTTAATCCACCGTATTTTTCGTGGAGGCA14420.10882969586552815No Hit
TCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACACT13940.1052070707604343No Hit
TAATTGTACTACTCATGGTAGTAACATCCAATGCAGATCGAATCTGCACT13740.10369764363331185No Hit
CTTATAGACAGATGGAGCAAGAAACATTGTCTCTGGAGACCATATAAACA13600.10264104464432615No Hit
TTACTACACAGGGGAACATGCAAAGGCCATAGGAAATTGCCCAATATGGG13580.1024901019316139No Hit
TCTCAGAGCTGATACAATAAGCTCACAAATAGAACTCGCAGTCCTGCTTT13450.10150897429898431No Hit
CTGTAGAACATGCTTGTAAATTCAAACATTCCAGTCTCATTACAGTAACT13370.10090520344813532No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACGCA1400.0134.480652
AGCACGC1450.0129.84341
CACGCAC1550.0126.1381763
TGACGCC404.791218E-9108.83254145
GTAACAA10250.096.079234
CGCACCC700.093.28505145
GCCGACC259.35917E-487.06604145
CGCACTT2200.085.5818255
GCTGCCC451.2275559E-680.6167145
ATAAGCC1000.079.81054145
AGTCAAT1050.075.860882
ACGCACT2600.075.197764
AAACACG4200.074.1395655
AGACGCC502.2962777E-672.55503145
CTCGGCC300.001930062772.55503145
CGTAATT300.001930062772.55503145
AACACGA3350.071.345336
GAAACAC4550.068.4339454
GAGCAGA1400.067.2403261
CAATTTA1200.066.3807755
Produced by FastQC (version 0.11.5)