FastQCFastQC Report
Thu 11 Jul 2019
HWC5JBCX2_l02_n02_SM2_9815_P2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l02_n02_SM2_9815_P2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences278465
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG8680.31170883234876917No Hit
GTATACTAGAGTCCCGTTTCCGTTTCATTACCAACACCACGTCTCCTTGC8460.30380837807264827No Hit
CTTTTAAGGCCGTGTTTGAAGAGTCGATAGACGCATTTGAAAAAAAGACG8210.29483058912251087No Hit
GCTATAACTATTTTAATGCATGTGTAAGGAAGGAGTTGAACCAAGACGCA7390.26538344136606035No Hit
ATTATATTCAGCATGGAAAGAATAAAAGAACTACGGAATCTAATGTCGCA7040.2528145368358681No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT6020.21618515791930765No Hit
GTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCCTGCACCATCTG5560.1996660262510549No Hit
TTTTTGGACAGTACGGATAACAAATAGTAGCACTGCTATAACTATTTTAA5230.1878153448368736No Hit
TTCCAACACTGTGTCAAGTTTCCAGGTAGATTGCTTTCTTTGGCATATCC5050.18135133679277468No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCACGGGGCCAAAGAAATAG4970.17847844432873072No Hit
TTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATC4790.1720144362846318No Hit
TTTTTAAACTATTCAGTATTAATTGATGGCCATCCGAATTCTTTTGGTCG4380.15729086240640655No Hit
ATCCCATATTATCCATGTTCTCATTTGAAGCAATTTGTACTCCTCTAGTT4290.1540588583843571No Hit
TATCAAAAGAGGGCACGATCGGGTTCGTTGCCTTCTCGTCTGAGAGCTCG4240.15226330059432963No Hit
TTCTTAGACAGAACCCGACTGAAGAACAAGCTGTGGATATATGCAAGGCT4240.15226330059432963No Hit
ATATACAGGAGAGTCGATGGAAAATGGATGAGGGAACTCGTCCTTTATGA4220.15154507747831863No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCC4000.14364462320219776No Hit
GTATTATTATCTCCTGAGGAGGTCAGTGAAACACAGGGAACTGAAAGACT3900.1400535076221428No Hit
GTCTAAGAATGTCCACCATCCTTGTTCCGCCAATTTGCGTGCTGTGGCAC3860.13861706139012084No Hit
ATTTTAATGCATGTGTAAGGAAGGAGTTGAACCAAGACGCATTGAGCAAA3780.13574416892607688No Hit
CTTCTGCATTGTCTCCGAAGAAATAAGATCCTTCGTTACTCATATCAAAA3700.13287127646203292No Hit
CTCATAGACTCAGGTACTCCTTCCGTAGAAGGGCCTCTTTTAAGGCCGTG3690.13251216490402742No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT3570.1282028262079615No Hit
CCTCAATAGTATGTCCTGGAAAAGAAGGCAATGGTGAGATTTCGCCAACA3540.127125491533945No Hit
ATTCAGTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT3420.12281615283787908No Hit
AGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCTTCAACCC3360.12066148348984614No Hit
CCTATATGAACTACTAGGGAAAAATTTCTCGAACAAGTTGCAGCACTTCT3330.11958414881582963No Hit
ATACAGGAGAGTCGATGGAAAATGGATGAGGGAACTCGTCCTTTATGACA3330.11958414881582963No Hit
CTCAATATCAGTGCAGATCTTGCTAAAAATATGAGATCTTCGATCTCAGC3220.1156339216777692No Hit
ATATGGGATCGAGCACTCTTGAACTGAGAAGCGGGTACTGGGCCATAAGG3200.1149156985617582No Hit
GATATTGAGAGGATCAGTTGCTCACAAATCTTGCCTACCTGCCTGTGCAT3200.1149156985617582No Hit
ATATAATGGGATCCCTTCTCCTTCGTGACTTGGGTCTTCAATGCTCAATT3190.11455658700375271No Hit
GAATAAAAGAACTACGGAATCTAATGTCGCAGTCTCGCACTCGCGAGATA3110.11168369453970876No Hit
CCCTAGAAGGTCCGGAGCTGCAGGTGCTGCAGTCAAAGGAATAGGAACAA3110.11168369453970876No Hit
ATCTAATGTCGCAGTCTCGCACTCGCGAGATACTGACAAAAACCACAGTG3000.10773346740164831No Hit
GTTCATTGATGCTTAATGCTGGCCCGTATCTTCTGTCTTCTTTACCTATA2990.10737435584364283No Hit
CCTTAGTAACATATCTCCTATCTCAAGGACACAATATTTCTCCCATTTAT2920.10486057493760437No Hit
TTACTAAGGAGTGCCATAGGCCAAATTTCAAGGCCGATGTTCTTGTATGT2890.10378324026358789No Hit
CATTAACACTGCCCTGCTCAATGCATCCTGTGCAGCAATGGACGATTTTC2850.10234679403156591No Hit
GTATTGGCTTCTAGTGGCCTTGGGGACTAAAGATTGAAATGGTTCAAATT2840.10198768247356041No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC2830.10162857091555492No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGCC100.007076523145.0795145
GCTGTCC100.007076523145.0795145
ATCGACC202.1220767E-6145.0795145
CGAAAGC750.0135.188693
GTGGGCC203.8546757E-4108.80962145
GCAGGGG352.7711212E-7103.4793249
CAGGGTG301.6044954E-596.58079
AGCGAAA1100.092.174111
GGTTAAT554.1836756E-1092.174112
GTACTGA651.6370905E-1189.151426
GGGTTAA608.3309715E-1084.492941
GCAGGGT554.409958E-879.020578
GGATCCT651.551598E-978.11973145
TACTGAT755.638867E-1177.264567
ACTATTC1600.072.4355248
GAATGTC1100.072.4355247
GTTAATA702.8267095E-972.422523
TATATTC1800.068.399043
TTATATT1800.068.399042
AAACTAT1700.068.1746146
Produced by FastQC (version 0.11.5)