FastQCFastQC Report
Thu 11 Jul 2019
HWC5JBCX2_l02_n02_SM2_9815_P2_DM2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l02_n02_SM2_9815_P2_DM2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1987313
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG59680.3003049846702558No Hit
GTATACTAGAGTCCCGTTTCCGTTTCATTACCAACACCACGTCTCCTTGC59200.2978896630777336No Hit
CTTTTAAGGCCGTGTTTGAAGAGTCGATAGACGCATTTGAAAAAAAGACG58410.2939144462900409No Hit
ATTATATTCAGCATGGAAAGAATAAAAGAACTACGGAATCTAATGTCGCA51400.25864068719924843No Hit
GCTATAACTATTTTAATGCATGTGTAAGGAAGGAGTTGAACCAAGACGCA51330.25828845280033896No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT41320.20791893375628295No Hit
GTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCCTGCACCATCTG39670.19961626578198802No Hit
TTCCAACACTGTGTCAAGTTTCCAGGTAGATTGCTTTCTTTGGCATATCC35220.1772242218513138No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCACGGGGCCAAAGAAATAG34820.17521145385754536No Hit
TTTTTGGACAGTACGGATAACAAATAGTAGCACTGCTATAACTATTTTAA34280.1724942170659579No Hit
TTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATC31710.15956218270599548No Hit
TTCTTAGACAGAACCCGACTGAAGAACAAGCTGTGGATATATGCAAGGCT31580.15890803310802074No Hit
ATATACAGGAGAGTCGATGGAAAATGGATGAGGGAACTCGTCCTTTATGA29680.1493473851376205No Hit
ATCCCATATTATCCATGTTCTCATTTGAAGCAATTTGTACTCCTCTAGTT29670.14929706593777628No Hit
TTTTTAAACTATTCAGTATTAATTGATGGCCATCCGAATTCTTTTGGTCG29060.14622759474727937No Hit
TATCAAAAGAGGGCACGATCGGGTTCGTTGCCTTCTCGTCTGAGAGCTCG27860.14018929076597395No Hit
ATTTTAATGCATGTGTAAGGAAGGAGTTGAACCAAGACGCATTGAGCAAA26420.13294332598840747No Hit
CTTCTGCATTGTCTCCGAAGAAATAAGATCCTTCGTTACTCATATCAAAA26390.13279236838887484No Hit
GTATTATTATCTCCTGAGGAGGTCAGTGAAACACAGGGAACTGAAAGACT25900.13032672759650843No Hit
CTCATAGACTCAGGTACTCCTTCCGTAGAAGGGCCTCTTTTAAGGCCGTG25630.12896810920071472No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT25620.12891779000087053No Hit
GTCTAAGAATGTCCACCATCCTTGTTCCGCCAATTTGCGTGCTGTGGCAC24930.12544576521161993No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCC24060.12106799482517348No Hit
GATATTGAGAGGATCAGTTGCTCACAAATCTTGCCTACCTGCCTGTGCAT23720.11935714203047029No Hit
CCTCAATAGTATGTCCTGGAAAAGAAGGCAATGGTGAGATTTCGCCAACA23530.11840107723343027No Hit
AGCAGGTACTGATTCAAAATGGAAGATTTTGTGCGACAATGCTTCAACCC23490.11819980043405341No Hit
ATTCAGTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT23070.11608639404059652No Hit
ATATGGGATCGAGCACTCTTGAACTGAGAAGCGGGTACTGGGCCATAAGG22380.11261436925134591No Hit
CTCAATATCAGTGCAGATCTTGCTAAAAATATGAGATCTTCGATCTCAGC22040.1109035164566427No Hit
CCTATATGAACTACTAGGGAAAAATTTCTCGAACAAGTTGCAGCACTTCT21870.1100480900592911No Hit
CCCTAGAAGGTCCGGAGCTGCAGGTGCTGCAGTCAAAGGAATAGGAACAA21690.10914234446209531No Hit
ATCTAATGTCGCAGTCTCGCACTCGCGAGATACTGACAAAAACCACAGTG21580.10858883326380897No Hit
TTACTAAGGAGTGCCATAGGCCAAATTTCAAGGCCGATGTTCTTGTATGT20810.10471425487580466No Hit
GAATAAAAGAACTACGGAATCTAATGTCGCAGTCTCGCACTCGCGAGATA20560.10345627487969937No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGGTACA20470.10300340208110145No Hit
CCTTAGTAACATATCTCCTATCTCAAGGACACAATATTTCTCCCATTTAT20320.1022486140834383No Hit
ATACAGGAGAGTCGATGGAAAATGGATGAGGGAACTCGTCCTTTATGACA20180.10154414528561932No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGTAT100.0071088346144.880847
TGACGCC600.0120.9227145
ACCGACC501.8189894E-12116.08579145
GCTGACC2500.0116.08579145
GGTCTTT1150.0113.562195145
GAGCACC501.9463187E-10101.57507145
GAAGCCC1150.094.63516145
CGACTCC700.093.283226145
AGCGAAA4750.093.012371
CTCGGCC554.1472958E-1092.340965145
GCTGTCC554.1472958E-1092.340965145
ATCGACC502.2582753E-887.06435145
GCAATCC702.910383E-1182.91842145
TCACGCC451.2279252E-680.615135145
GAGCGAA1750.078.635751
CGAAAGC5700.077.510313
ACAGACC1150.075.70813145
CCTATTT1600.072.55363145
ACCGAAA502.296967E-672.55362145
CAGGGGG607.99464E-872.55362145
Produced by FastQC (version 0.11.5)