FastQCFastQC Report
Thu 11 Jul 2019
HWC5JBCX2_l02_n02_SM2_9810_P2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l02_n02_SM2_9810_P2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences318036
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG15450.48579406104969247No Hit
CATTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAGCTCTGCTT11550.36316643398860504No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA11480.36096542529776504No Hit
CTACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAAT10300.32386270736646167No Hit
ACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGC9020.2836156913053868No Hit
GTAAGAAATACAGTAAAAATTGAATTTAATGCGATTCTTCTACCTCCAAA9010.28330126149240964No Hit
GTATGAAAAGTTCCTCAGAGAGCAACTGTCCGAAATACAGTTGGATTGAT8860.27858481429775245No Hit
GCTTTAGCACTTCCATTACATCCTTTGCAATTCCTTCCCCATTTTTCTGA8670.27261064785118666No Hit
GATATACAGAAAGCACTAATTGGTGCCTCTATCTGCTTTTTAAAACCCAA8280.2603478851450779No Hit
ATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAG8020.2521727100076721No Hit
CTTTTCATACAGTATGGCTCAAACCCTTCAATTCCAGCAGATGGATTCAT7840.24651297337408346No Hit
ATATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTCAT7660.24085323674049477No Hit
GTTCATAGCTGAGACCATCTGCATTTCCCGTCTCACTCCTGGCACTGAGG7090.2229307374007974No Hit
CATTCACACAGAGCTCATAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGT7030.22104415852293452No Hit
GGTCATATGCATTCAATCTATGCAGAGTTGATAAGGACTTGTATCCATTG7030.22104415852293452No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT6730.2116112641336201No Hit
GTGTGAATGTGATGCTTGTTTTTCGCACAAAGCACAGAGTGTTCCTAGTT6580.2068948169389629No Hit
GGTCAAGACCGTCTAAACAGACTAAAGAGGAAATTAGAGTCAAGAATAAA6520.20500823806110002No Hit
GTCCTATTGTCCAAGCCATGAAATGGAGAGCTGATAAAATAAAAGAACAA6430.2021783697443057No Hit
GAGTGTTCCTAGTTTTACTTGCATTGAATAATTTCCAGGATTCAGGTACA6340.19934850142751134No Hit
GTATGGCTCAAACCCTTCAATTCCAGCAGATGGATTCATAAATAAAAGTA6220.19557534367178556No Hit
ATTACATCCTTTGCAATTCCTTCCCCATTTTTCTGACTTGCCCCAAGGGA6160.1936887647939227No Hit
CAATATGACCACAACACAAATTGAGGTGGGTCCGGGAGCAACCAATGCCA5900.18551358965651688No Hit
GAGCCATACTGTATGAAAAGTTCCTCAGAGAGCAACTGTCCGAAATACAG5890.1851991598435397No Hit
GAATAAAGGGGCCAAATAAAGAGACAATAAACAGAGAGGTATCAATTTTG5850.18394144059163114No Hit
GATTCTGGCTGAGAGAAAAATGAGAAAATGTGTGAGCTTTCATGAAGCAT5740.18048271264888252No Hit
GCTATGAACACAGCAAAAACAATGAATGGAATGGGGAAAGGAGAAGACGT5650.1776528443320882No Hit
TATCCAATGATGACCAATAACCCCATGGACATCTTCGGAGCTTATGTGTA5490.17262196732445384No Hit
CTGTAAGATCATCAGTAGCAACAAGTTTAGCAACAAGCCTTCCACTCTGG5390.16947766919468238No Hit
CTCTTGAAGAGAGAAAAGCAATTGGAGTAAAAATGATGAAAGTACTTTTA5320.16727666050384232No Hit
GCTATGAGCTCTGTGTGAATGTGATGCTTGTTTTTCGCACAAAGCACAGA5290.1663333710649109No Hit
CTCCAATGTTGCTTTGCAGTTCTTCTGCCAGTTTTTGGACGTCTTCTCCT5240.16476122200002516No Hit
GCTCATAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGTGAGACGGGAAAT5190.16318907293513943No Hit
ACATTACGCATATCCCTTCTTATTGTCAAACGGAACTTCCCTTCTTTCTG5160.162245783496208No Hit
GTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAGTAAAA5130.16130249405727654No Hit
GTCATGTACCTGAATCCTGGAAATTATTCAATGCAAGTAAAACTAGGAAC5050.15878705555345934No Hit
GCCTTGGAATGGATAAAAAACAAAAGATGCTTAACTGATATACAGAAAGC5000.1572149064885736No Hit
GTTCAAACATTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAGC4890.15375617854582502No Hit
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG4830.15186959966796212No Hit
GTATTGTCCCTGAGAGTATTAGTAAACGGAACATTCCTCAAACACCCCAA4790.15061188041605353No Hit
TGACTACCCTGGTCAAGACCGTCTAAACAGACTAAAGAGGAAATTAGAGT4710.14809644191223637No Hit
GGTTCAAACATTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAG4670.14683872266032777No Hit
CTATTGTCCAAGCCATGAAATGGAGAGCTGATAAAATAAAAGAACAAATT4650.14620986303437347No Hit
CAATACTCCAATGTTGCTTTGCAGTTCTTCTGCCAGTTTTTGGACGTCTT4590.14432328415651058No Hit
ATCTAATGATGACCAATAACCCCATGGACATCTTCGGAGCTTATGTGTAC4550.14306556490460198No Hit
TCTGTGACCAGTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTT4360.1370913984580362No Hit
CCATTACATCCTTTGCAATTCCTTCCCCATTTTTCTGACTTGCCCCAAGG4290.13489038976719617No Hit
GTCCAAGCCATGAAATGGAGAGCTGATAAAATAAAAGAACAAATTGAAAG4260.13394710032826473No Hit
ACTCTGTGCTTTGTGCGAAAAACAAGCATCACATTCACACAGAGCTCATA4190.1317460916374247No Hit
TTGCAATTCCTTCCCCATTTTTCTGACTTGCCCCAAGGGATCTCAATACT4070.12797293388169892No Hit
TACCTATACAGGCAGCAATTTCAACAACATTCCCATACACCGGTGTTCCC4040.1270296444427675No Hit
CAACAAAAGTGAGCCTGAAAGTAAAAGGATGTCTCTTGAAGAGAGAAAAG3880.12199876743513313No Hit
CTCAGGGACAATACATTACGCATATCCCTTCTTATTGTCAAACGGAACTT3830.12042661837024739No Hit
TCTTCATCCTCCACTGTAAGATCATCAGTAGCAACAAGTTTAGCAACAAG3800.11948332893131595No Hit
GCACAGAGTGTTCCTAGTTTTACTTGCATTGAATAATTTCCAGGATTCAG3760.11822560967940736No Hit
CTCTTAAGGTCTGCCGCCACTGCCACTCCATGTGCTCCGTGAGATGTGTA3570.11225144323284157No Hit
TTCACACAGAGCTCATAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGTGA3560.11193701341986441No Hit
GCCCCAAGGGATCTCAATACTCCAATGTTGCTTTGCAGTTCTTCTGCCAG3560.11193701341986441No Hit
GCGTAATGTATTGTCCCTGAGAGTATTAGTAAACGGAACATTCCTCAAAC3480.10942157491604723No Hit
CTTCCATTACATCCTTTGCAATTCCTTCCCCATTTTTCTGACTTGCCCCA3450.1084782854771158No Hit
CATATGACCAGAGTGGAAGGCTTGTTGCTAAACTTGTTGCTACTGATGAT3430.1078494258511615No Hit
TACTAATACTCTCAGGGACAATACATTACGCATATCCCTTCTTATTGTCA3420.10753499603818435No Hit
GTAAAAGGATGTCTCTTGAAGAGAGAAAAGCAATTGGAGTAAAAATGATG3400.10690613641223007No Hit
TCATTAAGACGCTCGAAGAGTGAATTGAGGATCCGATGGCCATCTTCTTC3220.10124639977864142No Hit
AGCAGGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT3210.10093196996566425No Hit
AAATTACACTGTTGGTTCGGTGGGAAAGAATTTGACCTAGACTCTGCCTT3200.10061754015268712No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCGT100.007064034145.16844145
GGCACCC100.007064034145.16844145
CGCACCC100.007064034145.16844145
ATCACAC100.007110735144.848891
CGGTGCA100.007110735144.848892
GGTGCAT100.007110735144.848893
AGCACGC1000.0130.364011
AGCGTTT203.8723875E-4108.687948
CACGCAC1200.0108.636673
GCACGCA1200.0108.636672
ATAATAC406.1458377E-790.5305563
ACGCACT1650.087.787224
GAGCGTT259.402111E-486.9503567
CGCACTT1700.085.205235
TCTCGGG353.4139375E-582.9534145
GAAGCGG451.2386026E-680.471611
ACAACGC608.045754E-872.4586268
CAACGCA608.045754E-872.4586269
AACACGT406.6750516E-572.4586266
GAGCAGA406.690677E-572.4244461
Produced by FastQC (version 0.11.5)