FastQCFastQC Report
Tue 2 Jul 2019
HWC5JBCX2_l02_n02_SM2_9752_P2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l02_n02_SM2_9752_P2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1175604
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT34510.2935512298358971No Hit
CTCTTATCCTCTCTCTCTCCCACTCTGTCTCTCATCCTCTCTCTCTCCCA29690.25255102908802624No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACA28720.24429995134416013No Hit
CCTTAGACCAGTCATTGTCAAAATGTCGTTCAGAGTAAGAGAATCGCGCT28710.24421488868700686No Hit
CCATTAGGCAATACTCTTCCCCTCTGCTACCTGTGCACTCCAGCTGCCTC28500.2424285728867884No Hit
GTCTATGATTGTGTATGATAACAATGAGTCACTCTTTTGGACATGGAGGA28460.2420883222581754No Hit
GTATTACTGTCTATGATTGTGTATGATAACAATGAGTCACTCTTTTGGAC20520.17454857247848765No Hit
ATATAACGCAGTATAATCAAAACAACTGACTCTTGCCCCGAATCTGCCAT20010.17021037696367144No Hit
GGGCTAATCCTGTGCATGCAGCACGACACGAAGAACACCGACAAACTGGA18870.16051323404819992No Hit
CTCTATAATCTGCGCTGGAAATCGGTGGCCTACACGCCGCACCGCTCCAA18620.15838666761936843No Hit
CAATATACTAGGGGAAAGAAGTAAAAGGACAGCGAGGGGAAATGTGTACT18550.15779122901929563No Hit
GTATTCATCATCTTTGAGCAAATTCAGCTCTCGGAGAGGAAGGAAAAGCA18500.15736591573352932No Hit
CTCCCACTCTGTCTCTCATTCTCTCTCTCCATCCCATTCTCTCTCTCTCT17180.14613764498929913No Hit
GTACATGGACACCGGGCTGGTGCGGTTCGGAGAGCCGCCGCAGGAACCGA16500.1403533843028775No Hit
GTATAGTGTTGAGTGAGAGTGCAGGCATTACTCCGATAAATAAAGAATGA16370.13924756975988514No Hit
CTCCCACTCTGTCTCTCATCCTCTCTCTCTCCCACTCTGTCTCTCATTCT16370.13924756975988514No Hit
CTCTTGTGGTGTTTATGCAAAAGCCGGTCACGTGAAGTCTGCAAATGTAC15940.13558987550229498No Hit
GGTACTGACAGAGGAACGATGGAGACGGAGAGACAATATACTAGGGGAAA15670.133293183759157No Hit
GACAATATACTAGGGGAAAGAAGTAAAAGGACAGCGAGGGGAAATGTGTA15530.13210230655901137No Hit
GTATATCAGAAATCAATGATATCGATGTAGCACAGACACGCTGACCCTGA15150.12886992558718752No Hit
CTCCCTCTCTTATCCTCTCTCTCTCCCACTCTGTCTCTCATCCTCTCTCT14970.12733879775842888No Hit
ATACTAGGGGAAAGAAGTAAAAGGACAGCGAGGGGAAATGTGTACTTTAA14720.12521223132959738No Hit
GTATAATCAAAACAACTGACTCTTGCCCCGAATCTGCCATGAGATTTCAT14600.12419147944375827No Hit
GATATAACGCAGTATAATCAAAACAACTGACTCTTGCCCCGAATCTGCCA14570.1239362914722985No Hit
ATATACTAGGGGAAAGAAGTAAAAGGACAGCGAGGGGAAATGTGTACTTT14560.12385122881514524No Hit
GGACAAGACAGTGTAACCAGTCAGAGCAGTCGCAGAGCAGGGACATAAGC13910.11832215610018339No Hit
CTATCATTCATGTCTTGGGTCCTCAAAGGACCACCGGTTCAAACACATTT13870.11798190547157036No Hit
CTGTATTACTGTCTATGATTGTGTATGATAACAATGAGTCACTCTTTTGG13840.11772671750011059No Hit
GCTCTACATCACTGAAGGAGACAGTGACGAGGTGAACAACGATCATGAAG13500.11483458715689979No Hit
GGGAAGAGAGGAAGAGAAAAAGAATGGAAAGAAAAAGTGAGTGAAATGGT13500.11483458715689979No Hit
ATACAGCAGTGTGCAAACAACACCAAGGCTTCAATTACCCAACAAGCACA13100.11143208087076942No Hit
GTCTGTGATGTATTATCACACCACAGGCGGCACATTGTATATCAGAAATC12880.10956070241339771No Hit
TTATTACACACTCTATAATCTGCGCTGGAAATCGGTGGCCTACACGCCGC12770.10862501318471185No Hit
GGAGAGGAGGGACAGGGAAGAGAGGAAGAGAAAAAGAATGGAAAGAAAAA12330.10488225626996846No Hit
GTCTCTCATCCTCTCTCTCTCCCACTCTGTCTCTCATTCTCTCTCTCCAT12310.10471213095566193No Hit
ATGTAGCACAGACACGCTGACCCTGAAGAGCAGCTTGTTTATTCTCAGCA12190.10369137906982283No Hit
GTGGGAAGGAGAGGAGGGACAGGGAAGAGAGGAAGAGAAAAAGAATGGAA12190.10369137906982283No Hit
GGACAGGGAAGAGAGGAAGAGAAAAAGAATGGAAAGAAAAAGTGAGTGAA12130.10318100312690327No Hit
CTCTCCCACTCTGTCTCTCATCCTCTCTCTCTCCCACTCTGTCTCTCATT12090.10284075249829025No Hit
CTTTTAATCATGCTCCATGCAGGTACACATACCGACTGGCTCTACAAGGT12030.1023303765553707No Hit
TTCCAGCGCTGCGTGGACCAGATCCCTGAGCATCTCTCCGACGTCCCCCT11860.10088431138376527No Hit
ATTTCTCTCTCTCCCTCTCCCTCTCTTATCCTCTCTCTCTCCCACTCTGT11800.10037393544084572No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCGGG151.2261048E-4145.11725145
AGCTTCC404.789399E-9108.83794145
TAAGCCC301.5931677E-596.744835145
TTAGGCT406.1633E-790.513114
GTAATTT1600.081.628456145
GCGTCGG754.8912625E-967.603256
GGCGTCG754.9021764E-967.585995
GGACAGT2050.067.132156
TTTCGCC1950.066.977196145
TAGCTGG902.8376235E-1064.386799
CACGAGA350.003585365862.060852
CGACGGG1200.060.465523145
TTCTAGT3550.059.1471942
CGAAAGC30250.058.644023
TGTCACC3600.058.3480388
CCCAGGT3000.057.945648
CCTAAAC4350.054.9297523
ACCGTGC400.00607363654.3240368
TTAGTAG400.006082842654.3032462
GTTCTAG3950.053.1576041
Produced by FastQC (version 0.11.5)