FastQCFastQC Report
Thu 11 Jul 2019
HWC5JBCX2_l02_n02_SM2_9752_P2_DM2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l02_n02_SM2_9752_P2_DM2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1181438
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT34610.29294808529944016No Hit
CTCTTATCCTCTCTCTCTCCCACTCTGTCTCTCATCCTCTCTCTCTCCCA29800.2522349882092839No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACA28850.24419393992744434No Hit
CCTTAGACCAGTCATTGTCAAAATGTCGTTCAGAGTAAGAGAATCGCGCT28830.24402465470045823No Hit
CCATTAGGCAATACTCTTCCCCTCTGCTACCTGTGCACTCCAGCTGCCTC28580.24190858936313206No Hit
GTCTATGATTGTGTATGATAACAATGAGTCACTCTTTTGGACATGGAGGA28490.24114680584169462No Hit
GTATTACTGTCTATGATTGTGTATGATAACAATGAGTCACTCTTTTGGAC20560.1740252133417073No Hit
ATATAACGCAGTATAATCAAAACAACTGACTCTTGCCCCGAATCTGCCAT20070.16987772528054795No Hit
GGGCTAATCCTGTGCATGCAGCACGACACGAAGAACACCGACAAACTGGA18910.16005918211535433No Hit
CTCTATAATCTGCGCTGGAAATCGGTGGCCTACACGCCGCACCGCTCCAA18680.15811240200501422No Hit
CAATATACTAGGGGAAAGAAGTAAAAGGACAGCGAGGGGAAATGTGTACT18590.1573506184835768No Hit
GTATTCATCATCTTTGAGCAAATTCAGCTCTCGGAGAGGAAGGAAAAGCA18550.15701204802960458No Hit
CTCCCACTCTGTCTCTCATTCTCTCTCTCCATCCCATTCTCTCTCTCTCT17210.14566993782153612No Hit
GTACATGGACACCGGGCTGGTGCGGTTCGGAGAGCCGCCGCAGGAACCGA16560.14016816794448797No Hit
GTATAGTGTTGAGTGAGAGTGCAGGCATTACTCCGATAAATAAAGAATGA16410.13889852874209227No Hit
CTCCCACTCTGTCTCTCATCCTCTCTCTCTCCCACTCTGTCTCTCATTCT16410.13889852874209227No Hit
CTCTTGTGGTGTTTATGCAAAAGCCGGTCACGTGAAGTCTGCAAATGTAC16000.1354281815888773No Hit
GGTACTGACAGAGGAACGATGGAGACGGAGAGACAATATACTAGGGGAAA15740.13322747363805804No Hit
GACAATATACTAGGGGAAAGAAGTAAAAGGACAGCGAGGGGAAATGTGTA15560.13170390659518316No Hit
GTATATCAGAAATCAATGATATCGATGTAGCACAGACACGCTGACCCTGA15190.12857212989594038No Hit
CTCCCTCTCTTATCCTCTCTCTCTCCCACTCTGTCTCTCATCCTCTCTCT14990.1268792776260794No Hit
ATACTAGGGGAAAGAAGTAAAAGGACAGCGAGGGGAAATGTGTACTTTAA14740.1247632122887532No Hit
GTATAATCAAAACAACTGACTCTTGCCCCGAATCTGCCATGAGATTTCAT14660.12408607138080881No Hit
GATATAACGCAGTATAATCAAAACAACTGACTCTTGCCCCGAATCTGCCA14600.12357821569985053No Hit
ATATACTAGGGGAAAGAAGTAAAAGGACAGCGAGGGGAAATGTGTACTTT14590.12349357308635747No Hit
GGACAAGACAGTGTAACCAGTCAGAGCAGTCGCAGAGCAGGGACATAAGC13930.11790716059581628No Hit
CTATCATTCATGTCTTGGGTCCTCAAAGGACCACCGGTTCAAACACATTT13910.11773787536883018No Hit
CTGTATTACTGTCTATGATTGTGTATGATAACAATGAGTCACTCTTTTGG13870.11739930491485799No Hit
GCTCTACATCACTGAAGGAGACAGTGACGAGGTGAACAACGATCATGAAG13540.1146060986695874No Hit
GGGAAGAGAGGAAGAGAAAAAGAATGGAAAGAAAAAGTGAGTGAAATGGT13540.1146060986695874No Hit
ATACAGCAGTGTGCAAACAACACCAAGGCTTCAATTACCCAACAAGCACA13160.11138967935685157No Hit
GTCTGTGATGTATTATCACACCACAGGCGGCACATTGTATATCAGAAATC12900.1091889714060323No Hit
TTATTACACACTCTATAATCTGCGCTGGAAATCGGTGGCCTACACGCCGC12810.10842718788459489No Hit
GGAGAGGAGGGACAGGGAAGAGAGGAAGAGAAAAAGAATGGAAAGAAAAA12390.10487219811788685No Hit
GTCTCTCATCCTCTCTCTCTCCCACTCTGTCTCTCATTCTCTCTCTCCAT12350.10453362766391465No Hit
ATGTAGCACAGACACGCTGACCCTGAAGAGCAGCTTGTTTATTCTCAGCA12240.10360255891549111No Hit
GTGGGAAGGAGAGGAGGGACAGGGAAGAGAGGAAGAGAAAAAGAATGGAA12230.10351791630199808No Hit
GGACAGGGAAGAGAGGAAGAGAAAAAGAATGGAAAGAAAAAGTGAGTGAA12190.10317934584802588No Hit
CTCTCCCACTCTGTCTCTCATCCTCTCTCTCTCCCACTCTGTCTCTCATT12120.10258684755357454No Hit
CTTTTAATCATGCTCCATGCAGGTACACATACCGACTGGCTCTACAAGGT12070.10216363448610931No Hit
TTCCAGCGCTGCGTGGACCAGATCCCTGAGCATCTCTCCGACGTCCCCCT11920.10089399528371357No Hit
ATTTCTCTCTCTCCCTCTCCCTCTCTTATCCTCTCTCTCTCCCACTCTGT11820.1000475691487831No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGCTC501.9463187E-10101.55578145
TAGCGGG259.366705E-487.04781145
AGCTTCC451.229002E-680.59982145
GGACAGT1350.080.50776
GGTGCCC608.002462E-872.53984145
TTCTAGT2700.069.7556152
CGACGGG754.834874E-967.70385145
CGAAAGC29250.064.1475753
TGTCACC2950.063.863048
CGAGCCC2200.062.5764356
GGCCCTA2500.060.8483621
CAAGGCC851.3000317E-859.738697145
GCGAAAG31850.058.678612
TACGAGC2350.058.5747574
CTCGCGG1007.221388E-1057.97299
GTTCTAG3250.057.9508171
GATGTGA502.0188412E-457.9508171
TTAGATG2550.056.8193363
AGCGAAA32900.056.585721
TCGCGCC651.0902122E-555.799877145
Produced by FastQC (version 0.11.5)