FastQCFastQC Report
Thu 11 Jul 2019
HWC5JBCX2_l02_n02_SM2_9730_P2_DM2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l02_n02_SM2_9730_P2_DM2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1510698
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT76830.5085728583740761No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC47750.316079057495277No Hit
GTAAGAAATACAGTAAAATTGAATTTAATGCGATTCTTCTACTTCCAAAA44410.2939700721123613No Hit
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG36570.24207353157282263No Hit
CTACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAAT30880.20440882294144824No Hit
ATACTATACTGCTCTATTACTCAACTGCTGCTTCTAGTTTGGCTGTAACG30750.20354829357025692No Hit
GATATACAGAAAGCACTAATTGGTGCCTCTATCTGCTTTTTAAAACCCAA28960.19169946607462246No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC28600.18931646166209262No Hit
ACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGC25590.1693918969906626No Hit
CATTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAGCTCTGCTT25130.16634694690798557No Hit
CTATAGAACATGCTTGTAAATTCAAACATTCCAGTTTCGTTACAGTAACT24950.16515544470172067No Hit
GTAGTAACAAGAGCCTTTTTTTCATTTTAATCATTTGTCTATCACATGTA24380.16138235438188175No Hit
ATATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTCAT24260.16058801957770513No Hit
ATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAG23200.15357139547414506No Hit
GCTTTAGCACTTCCATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGA22090.1462237985355114No Hit
GTTCTATAGAGATGGATTTGTATCTAACTTTGCAATGGAGATTCCTTCAT19890.1316609937922735No Hit
CAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGCAAACAAG19850.13139621552421463No Hit
AGCAGGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT19050.12610065016303723No Hit
CATTCACACAGAGCTCATAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGT18520.12259233811125717No Hit
GTCCTATTGTCCAAGCCATGAAATGGAGAGCTGATAAAATAAAAGAACAA18510.12252614354424246No Hit
ATTACTATGTGGTCACTCAATACTTCCATGTTGTCAGAGAGTACTTCCTT18450.12212897614215416No Hit
GTTCATAGCTGAGACCATCTGCATTTCCCGTCTCACTCCTGGCACTGAGG17480.11570810314172654No Hit
CCTATAATGTTTGAACCATTCCAGATTCTTTCAATTTGTTCTTTTATTTT17040.11279554219307897No Hit
ATCCAATGATGACCAATAACCCCATGGACATCTTCGGAGCTTATGTGTAC16920.11200120738890235No Hit
GTGTGAATGTGATGCTTGTTTTTCGCACAAAGCACAGAGTGTTCCTAGTT16710.11061112148159327No Hit
GCCTTGGAATGGATAAAAAACAAAAGATGCTTAACTGATATACAGAAAGC16220.10736758769787211No Hit
GTATTTACACAGTCTCTCCAAAGTTTCCATAGGAAATTTTCGTTAGCTTT15950.10558033438847474No Hit
AGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACATTGATTACAAT15770.1043888321822098No Hit
GTATAGATCTGTTCCTTTTGGTTCTCCAACTGTGAGTTCCAGACACTGCA15600.10326352454295962No Hit
CTCTTAAGGTCTGCCGCCACTGCCACTCCATGTGCTCCGTGAGATGTGTA15590.10319732997594488No Hit
GTACTTCCTTCATTGCTTCTTTAGCCTGAATTTCTTTTTGGTAACTGTGT15440.10220441147072412No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATCAC100.0071164123144.82811
AGCACGC10400.0124.635711
GCACGCA10600.0122.967242
CACGCAC10750.0121.251413
ATAAGCC550.0118.707886145
TGACGCC501.9463187E-10101.56118145
CGGTGCA1300.0100.26562
GGCACCC800.099.74759145
AAACACG4950.099.494355
ACGCACT13700.098.852344
CGCACTT13700.098.330235
GTAACAA23000.098.240984
CGTGCTC301.5949445E-596.72494145
GAAACAC5350.092.049424
ACGTGCA2350.089.3620152
CGCACCC451.2287801E-680.60412145
GAAGCGG1900.076.22531
GGTGCAT1750.074.483023
AACACGT2150.074.117726
GCGGTGC2950.073.64141
Produced by FastQC (version 0.11.5)