FastQCFastQC Report
Tue 2 Jul 2019
HWC5JBCX2_l02_n02_SM2_9722_P2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l02_n02_SM2_9722_P2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2268857
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTTAAGGCCGTGTTTGAATAGTCGATAGACGCATTTGAAAAAAAGACG174450.7688893570639314No Hit
TTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATC123220.5430928436653346No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT117590.5182785869713252No Hit
GTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCCTGCACCATCTG115530.5091991253745829No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCATGGGGCCAAAGAAATAG88130.388433471126651No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCC84770.37362425221157614No Hit
CAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGTGTG77910.3433887635932983No Hit
CTCATAGACTCAGGTACTCCTTCCGTAGAAGGGCCTCTTTTAAGGCCGTG74370.32778619366491585No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT74100.3265961671449545No Hit
ATCCTATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATA69070.30442641382863705No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC62770.2766591283628717No Hit
GGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATCAGAAACGAAT61100.26929859396162914No Hit
TCCTAGTTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGA60680.2674474415972448No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA58220.25660497774870783No Hit
TTCCAACACTGTGTCAAGTTTCCAGGTAGATTGCTTTCTTTGGCATATCC58130.25620830224205404No Hit
CTCATGGAATGGCTAAAGACAAGACCAATTCTGTCACCTTTGACTAAGGG57550.25365194897695187No Hit
CTCTTAGACCAGTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCC53010.23364187341908282No Hit
TTACTAAGGAGTGCCATAGGCCAAATTTCAAGGCCGATGTTCTTGTATGT52470.2312618203791601No Hit
GACTTGAAGATGTCTTTGCTGGGAAAAACACAGATCTTGAGGCTCTCATG49520.2182596787721747No Hit
GCACTACAGCTAAGGCTATGGAGCAAATGGCTGGATCAAGTGAGCAGGCA48600.2142047735930471No Hit
GTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGAGAG48200.21244177134125244No Hit
ATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT44350.19547287466772917No Hit
TGTTTGAATAGTCGATAGACGCATTTGAAAAAAAGACGATCAAGAATCCA43370.19115351915083234No Hit
GGCCTGGTGTGTGCAACATGTGAGCAGATTGCTGATTCCCAGCACAGGTC42600.1877597398161277No Hit
TCTCTCTATCGTTCCATCAGGCCCCCTCAAAGCCGAGATCGCGCAGAGAC42420.1869663888028201No Hit
CATTAACACTGCCCTGCTCAATGCATCCTGTGCAGCAATGGACGATTTTC41480.18282333351110272No Hit
GCATTCTGCTGTTCCTTTCGATATTCTTCCCTCATAGACTCAGGTACTCC40960.18053143058376972No Hit
GTCGATAGACGCATTTGAAAAAAAGACGATCAAGAATCCACAATATCAAG40670.1792532539512186No Hit
CCTTAGTAACATATCTCCTATCTCAAGGACACAATATTTCTCCCATTTAT40280.17753432675571884No Hit
CACCAGGCCAAATGCCACTTCAGTGGTTACAGCCCCCATCCTATTGTATA40250.17740210158683425No Hit
GCTATAACTATTTTAGTGCATGTGTCAGGAAGGAGTTGAACCAAGACGCA40250.17740210158683425No Hit
ATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGT39360.17347942157659121No Hit
GGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAAACGTATGTTCT39200.17277422067587336No Hit
TAGTAATACTATGGTCTCTAGTCGGCCAAAAATCACATTGAAATTTGCTT38390.16920414111598925No Hit
CTGCTGTTCCTTTCGATATTCTTCCCTCATAGACTCAGGTACTCCTTCCG38010.16752928897678435No Hit
AAACAAGGTAGTTTTTTACTCCAACTCTATGCTGACAAAATGACTGTCGT37940.16722076358272028No Hit
CGTATGTTCTCTCTATCGTTCCATCAGGCCCCCTCAAAGCCGAGATCGCG37620.16581036178128458No Hit
TCCCAGCACAGGTCTCATAGGCAGATGGTGGCAACAACCAATCCATTAAT37070.16338623368506697No Hit
CCTCAATAGTATGTCCTGGAAAAGAAGGCAATGGTGAGATTTCGCCAACA36250.159772079068888No Hit
GGCTAAAGACAAGACCAATTCTGTCACCTTTGACTAAGGGGATTTTAGGG36150.1593313285059393No Hit
CTATCGTTCCATCAGGCCCCCTCAAAGCCGAGATCGCGCAGAGACTTGAA36130.15924317839334962No Hit
GCCTTAGCTGTAGTGCTGGCCAAAACCATTCTGTTCTCATGTTTTATTAA35890.1581853770422728No Hit
GCTAAAGACAAGACCAATTCTGTCACCTTTGACTAAGGGGATTTTAGGGT35790.15774462647932416No Hit
AGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGACAAAATGACT35380.15593754917123467No Hit
CCCAAATAACATGGACAAAGCAGTTAAACTGTATAGGAAACTTAAGAGGG33990.14981111634624836No Hit
CTCATAGGCAGATGGTGGCAACAACCAATCCATTAATAAAACATGAGAAC33760.14879739005146644No Hit
CAGGTACTCCTTCCGTAGAAGGGCCTCTTTTAAGGCCGTGTTTGAATAGT33270.14663771229301803No Hit
GTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATCTC33190.1462851118426591No Hit
GTCCCAATGGCTCTCATTGCCTGCACCATCTGCCTGGCCTGACTAGCAAT33020.14553583588564636No Hit
TTTTTGGACAGTACGGATAACAAATAGTAGCACTGCTATAACTATTTTAG33020.14553583588564636No Hit
GCCTATGAGACCTGTGCTGGGAATCAGCAATCTGCTCACATGTTGCACAC32750.14434580936568503No Hit
CTATTGAGGATGTCAAAAATGCAATTGGGGTCCTCATCGGAGGACTTGAA32050.1412605554250444No Hit
CATGAGAACAGAATGGTTTTGGCCAGCACTACAGCTAAGGCTATGGAGCA31460.13866012710364736No Hit
TCTTAGACCAGTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCCT30900.13619192395113489No Hit
CAGTTATTCTGCTGGTGCACTTGCCAGTTGCATGGGCCTCATATACAATA30830.1358833985570708No Hit
TTATTTGGGTCTCCATTCCCATTGAGGGCATTTTGGACAAAGCGTCTACG30740.135486723050417No Hit
GTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATCAGAAACGAATG30290.13350334551714807No Hit
ATCCCAATGATATTCGCGGCAACAACAAGCGGGTCATTTGAATCGTTGCA30130.13279814461643022No Hit
TCTGATAGGTCTGCAAATTTTCAAGAAGATCATCTCTTAGACCAGTACTG29930.13191664349053292No Hit
GAATAACTGAGAGCTATTTCTTTGGCCCCATGGAACGTTATCTCCCTCTT29920.13187256843423803No Hit
CTCCATGGCCTCCGCTGCCTGCTCACTTGATCCAGCCATTTGCTCCATAG29880.13169626820905858No Hit
CCACAATATCAAGTGCAAGATCCCAATGATATTCGCGGCAACAACAAGCG29730.1310351423646356No Hit
ATCAAGTGCAAGATCCCAATGATATTCGCGGCAACAACAAGCGGGTCATT29380.12949251539431528No Hit
GTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCCATTGAGGGCA29190.12865508932471284No Hit
GACTAGCAATCTCCATGGCCTCCGCTGCCTGCTCACTTGATCCAGCCATT29020.12790581336770013No Hit
CTCAGGTACTCCTTCCGTAGAAGGGCCTCTTTTAAGGCCGTGTTTGAATA28980.12772951314252065No Hit
TTCTTAATCTGTGTCTCACCTCTTCAATTAGCCATCTTATCTCTTCAAAC28920.12746506280475145No Hit
CTCTATCGTTCCATCAGGCCCCCTCAAAGCCGAGATCGCGCAGAGACTTG28800.12693616212921308No Hit
GTCCAAAATGCCCTCAATGGGAATGGAGACCCAAATAACATGGACAAAGC28610.1260987360596106No Hit
GTTTTTTACTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCAC28590.12601058594702091No Hit
GAACTAGGATGAGTCCCAATGGCTCTCATTGCCTGCACCATCTGCCTGGC28550.12583428572184144No Hit
GGTGTGTGCAACATGTGAGCAGATTGCTGATTCCCAGCACAGGTCTCATA28540.12579021066554658No Hit
CTCATTGCCTGCACCATCTGCCTGGCCTGACTAGCAATCTCCATGGCCTC27910.12301348211897004No Hit
TTGCTAGTCAGGCCAGGCAGATGGTGCAGGCAATGAGAGCCATTGGGACT27780.12244050638713679No Hit
GTGCTGGGAATCAGCAATCTGCTCACATGTTGCACACACCAGGCCAAATG27690.122043830880483No Hit
GGTCTCATAGGCAGATGGTGGCAACAACCAATCCATTAATAAAACATGAG27640.12182345559900866No Hit
GTTCTCATGTTTTATTAATGGATTGGTTGTTGCCACCATCTGCCTATGAG27180.11979600300944485No Hit
CTCATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCT26860.11838560120800913No Hit
GATCTTGAGGCTCTCATGGAATGGCTAAAGACAAGACCAATTCTGTCACC26480.11671074906880424No Hit
GTGTTATCATTCCATTCAAGTCCTCCGATGAGGACCCCAATTGCATTTTT26400.11635814861844533No Hit
GGCAATGAGAGCCATTGGGACTCATCCTAGTTCCAGTACTGGTCTAAGAG26270.11578517288661208No Hit
CCTTTGACTAAGGGGATTTTAGGGTTTGTTTTCACGCTCACCGTGCCCAG26240.11565294771772748No Hit
GTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGACAAAATGACTG25900.11415439580370204No Hit
CTGTAGTGCTGGCCAAAACCATTCTGTTCTCATGTTTTATTAATGGATTG25640.11300844434003553No Hit
CCATTGGGACTCATCCTAGTTCCAGTACTGGTCTAAGAGATGATCTTCTT25560.11265584388967662No Hit
GACCAATTCTGTCACCTTTGACTAAGGGGATTTTAGGGTTTGTTTTCACG25400.11195064298895877No Hit
CTATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACT25380.11186249287636903No Hit
AGCTAAGGCTATGGAGCAAATGGCTGGATCAAGTGAGCAGGCAGCGGAGG25170.11093691669417684No Hit
GTGCAACATGTGAGCAGATTGCTGATTCCCAGCACAGGTCTCATAGGCAG24950.1099672654556898No Hit
CCCCCATTCGTTTCTGATAGGTCTGCAAATTTTCAAGAAGATCATCTCTT24900.10974689017421548No Hit
CCTCAAAGCCGAGATCGCGCAGAGACTTGAAGATGTCTTTGCTGGGAAAA24730.10899761421720275No Hit
CCGTAGAAGGGCCTCTTTTAAGGCCGTGTTTGAATAGTCGATAGACGCAT24690.1088213139920233No Hit
GCTAAGGCTATGGAGCAAATGGCTGGATCAAGTGAGCAGGCAGCGGAGGC24310.1071464618528184No Hit
GTGCAGGCAATGAGAGCCATTGGGACTCATCCTAGTTCCAGTACTGGTCT24290.10705831174022866No Hit
ATTGAAAGATGAGCCTTCTAACCGAGGTCGAAACGTATGTTCTCTCTATC24280.10701423668393381No Hit
TATGAGACCTGTGCTGGGAATCAGCAATCTGCTCACATGTTGCACACACC23890.10529530948843405No Hit
CAATGGGAATGGAGACCCAAATAACATGGACAAAGCAGTTAAACTGTATA23720.10454603353142132No Hit
CCTTTGGGGGACTCCCCAATGGGCCATGCTTCTGATTTATTCTCAAAAAA23410.1031797067862805No Hit
GCTTTGTCCATGTTATTTGGGTCTCCATTCCCATTGAGGGCATTTTGGAC23100.10181338004113966No Hit
GTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATCAGAAA22970.1012404043093064No Hit
CAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGTTCCTTTCGATAT22780.10040297823970395No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTAA1000.0108.81675145
AGCGAAA6950.0102.130651
AGTAGAA9350.089.8589251
TGCGCCC1050.089.817145
GAGCGAA2050.088.328521
GACGTCC259.365438E-487.0534145
CGAAAGC8700.081.588933
CCTCGCC851.8189894E-1276.81182145
GGACAGT6800.069.247716
CAGTACG7450.068.0769659
ACAGTAC7850.064.602388
TAAACTA1150.062.9876335
TAGAAAC14050.060.831723
TCACGCC4600.059.928062145
AGGCGCC502.0047011E-458.0356145
CTTATAA502.0047011E-458.0356145
GACAGTA8400.057.7825247
ACCGACC651.0901196E-555.803463145
GTATTAA4050.055.4397851
GCAATCC1750.053.890198145
Produced by FastQC (version 0.11.5)