FastQCFastQC Report
Thu 11 Jul 2019
HWC5JBCX2_l02_n02_SM2_9722_P2_DM2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l02_n02_SM2_9722_P2_DM2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2279978
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTTAAGGCCGTGTTTGAATAGTCGATAGACGCATTTGAAAAAAAGACG174900.7671126651222073No Hit
TTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATC123610.5421543541209608No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT117870.5169786726012269No Hit
GTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCCTGCACCATCTG115860.5081627980620865No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCATGGGGCCAAAGAAATAG88370.38759145921583454No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCC84940.37254745440526177No Hit
CAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGTGTG78150.34276646529045457No Hit
CTCATAGACTCAGGTACTCCTTCCGTAGAAGGGCCTCTTTTAAGGCCGTG74550.32697683925020327No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT74390.3262750780928588No Hit
ATCCTATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATA69250.3037310009131667No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC62900.2758798549810568No Hit
GGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATCAGAAACGAAT61270.26873066319060973No Hit
TCCTAGTTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGA60810.2667130998632443No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA58330.2558358019244045No Hit
TTCCAACACTGTGTCAAGTTTCCAGGTAGATTGCTTTCTTTGGCATATCC58320.2557919418520705No Hit
CTCATGGAATGGCTAAAGACAAGACCAATTCTGTCACCTTTGACTAAGGG57650.2528533170056904No Hit
CTCTTAGACCAGTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCC53110.23294084416604022No Hit
TTACTAAGGAGTGCCATAGGCCAAATTTCAAGGCCGATGTTCTTGTATGT52620.23079170062167267No Hit
GACTTGAAGATGTCTTTGCTGGGAAAAACACAGATCTTGAGGCTCTCATG49600.21754595877679522No Hit
GCACTACAGCTAAGGCTATGGAGCAAATGGCTGGATCAAGTGAGCAGGCA48700.21359855226673238No Hit
GTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGAGAG48350.21206344973504132No Hit
ATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT44520.19526504203110734No Hit
TGTTTGAATAGTCGATAGACGCATTTGAAAAAAAGACGATCAAGAATCCA43480.19070359450836807No Hit
GGCCTGGTGTGTGCAACATGTGAGCAGATTGCTGATTCCCAGCACAGGTC42700.18728250886631362No Hit
TCTCTCTATCGTTCCATCAGGCCCCCTCAAAGCCGAGATCGCGCAGAGAC42520.18649302756430106No Hit
CATTAACACTGCCCTGCTCAATGCATCCTGTGCAGCAATGGACGATTTTC41630.18258948112657228No Hit
GCATTCTGCTGTTCCTTTCGATATTCTTCCCTCATAGACTCAGGTACTCC41120.18035261743753667No Hit
GTCGATAGACGCATTTGAAAAAAAGACGATCAAGAATCCACAATATCAAG40770.17881751490584558No Hit
GCTATAACTATTTTAGTGCATGTGTCAGGAAGGAGTTGAACCAAGACGCA40360.1770192519401503No Hit
CCTTAGTAACATATCTCCTATCTCAAGGACACAATATTTCTCCCATTTAT40340.17693153179548224No Hit
CACCAGGCCAAATGCCACTTCAGTGGTTACAGCCCCCATCCTATTGTATA40320.17684381165081417No Hit
ATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGT39450.17302798535775346No Hit
GGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAAACGTATGTTCT39350.17258938463441315No Hit
TAGTAATACTATGGTCTCTAGTCGGCCAAAAATCACATTGAAATTTGCTT38460.16868583819668437No Hit
CTGCTGTTCCTTTCGATATTCTTCCCTCATAGACTCAGGTACTCCTTCCG38090.16706301552032518No Hit
AAACAAGGTAGTTTTTTACTCCAACTCTATGCTGACAAAATGACTGTCGT38070.16697529537565714No Hit
CGTATGTTCTCTCTATCGTTCCATCAGGCCCCCTCAAAGCCGAGATCGCG37710.16539633277163202No Hit
TCCCAGCACAGGTCTCATAGGCAGATGGTGGCAACAACCAATCCATTAAT37110.16276472843159012No Hit
CCTCAATAGTATGTCCTGGAAAAGAAGGCAATGGTGAGATTTCGCCAACA36350.15943136293420374No Hit
CTATCGTTCCATCAGGCCCCCTCAAAGCCGAGATCGCGCAGAGACTTGAA36260.15903662228319745No Hit
GGCTAAAGACAAGACCAATTCTGTCACCTTTGACTAAGGGGATTTTAGGG36240.1589489021385294No Hit
GCTAAAGACAAGACCAATTCTGTCACCTTTGACTAAGGGGATTTTAGGGT35910.15750151975150636No Hit
GCCTTAGCTGTAGTGCTGGCCAAAACCATTCTGTTCTCATGTTTTATTAA35900.15745765967917236No Hit
AGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGACAAAATGACT35510.15574711685814513No Hit
CCCAAATAACATGGACAAAGCAGTTAAACTGTATAGGAAACTTAAGAGGG34110.14960670673138074No Hit
CTCATAGGCAGATGGTGGCAACAACCAATCCATTAATAAAACATGAGAAC33840.1484224847783619No Hit
CAGGTACTCCTTCCGTAGAAGGGCCTCTTTTAAGGCCGTGTTTGAATAGT33310.14609790094465824No Hit
GTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATCTC33260.14587860058298807No Hit
TTTTTGGACAGTACGGATAACAAATAGTAGCACTGCTATAACTATTTTAG33080.1450891192809755No Hit
GTCCCAATGGCTCTCATTGCCTGCACCATCTGCCTGGCCTGACTAGCAAT33070.1450452592086415No Hit
GCCTATGAGACCTGTGCTGGGAATCAGCAATCTGCTCACATGTTGCACAC32800.14386103725562263No Hit
CTATTGAGGATGTCAAAAATGCAATTGGGGTCCTCATCGGAGGACTTGAA32110.14083469226457448No Hit
CATGAGAACAGAATGGTTTTGGCCAGCACTACAGCTAAGGCTATGGAGCA31540.1383346681415347No Hit
TCTTAGACCAGTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCCT30970.1358346440184949No Hit
CAGTTATTCTGCTGGTGCACTTGCCAGTTGCATGGGCCTCATATACAATA30930.1356592037291588No Hit
TTATTTGGGTCTCCATTCCCATTGAGGGCATTTTGGACAAAGCGTCTACG30840.1352644630781525No Hit
GTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATCAGAAACGAATG30360.133159179606119No Hit
ATCCCAATGATATTCGCGGCAACAACAAGCGGGTCATTTGAATCGTTGCA30240.13263285873811062No Hit
TCTGATAGGTCTGCAAATTTTCAAGAAGATCATCTCTTAGACCAGTACTG30100.1320188177254342No Hit
GAATAACTGAGAGCTATTTCTTTGGCCCCATGGAACGTTATCTCCCTCTT30030.13171179721909598No Hit
CTCCATGGCCTCCGCTGCCTGCTCACTTGATCCAGCCATTTGCTCCATAG29980.1314924968574258No Hit
CCACAATATCAAGTGCAAGATCCCAATGATATTCGCGGCAACAACAAGCG29860.13096617598941745No Hit
ATCAAGTGCAAGATCCCAATGATATTCGCGGCAACAACAAGCGGGTCATT29490.12934335331305827No Hit
GTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCCATTGAGGGCA29290.12846615186637766No Hit
GACTAGCAATCTCCATGGCCTCCGCTGCCTGCTCACTTGATCCAGCCATT29040.1273696500580269No Hit
CTCAGGTACTCCTTCCGTAGAAGGGCCTCTTTTAAGGCCGTGTTTGAATA29030.12732578998569283No Hit
TTCTTAATCTGTGTCTCACCTCTTCAATTAGCCATCTTATCTCTTCAAAC29000.12719420976869075No Hit
CTCTATCGTTCCATCAGGCCCCCTCAAAGCCGAGATCGCGCAGAGACTTG28880.12666788890068237No Hit
GTCCAAAATGCCCTCAATGGGAATGGAGACCCAAATAACATGGACAAAGC28690.1258345475263358No Hit
GTTTTTTACTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCAC28680.12579068745400174No Hit
GGTGTGTGCAACATGTGAGCAGATTGCTGATTCCCAGCACAGGTCTCATA28670.1257468273816677No Hit
GAACTAGGATGAGTCCCAATGGCTCTCATTGCCTGCACCATCTGCCTGGC28610.12548366694766352No Hit
CTCATTGCCTGCACCATCTGCCTGGCCTGACTAGCAATCTCCATGGCCTC27990.12276434246295358No Hit
TTGCTAGTCAGGCCAGGCAGATGGTGCAGGCAATGAGAGCCATTGGGACT27870.12223802159494522No Hit
GTGCTGGGAATCAGCAATCTGCTCACATGTTGCACACACCAGGCCAAATG27790.12188714101627296No Hit
GGTCTCATAGGCAGATGGTGGCAACAACCAATCCATTAATAAAACATGAG27780.12184328094393894No Hit
GTTCTCATGTTTTATTAATGGATTGGTTGTTGCCACCATCTGCCTATGAG27300.11973799747190543No Hit
CTCATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCT26970.1182906150848824No Hit
GATCTTGAGGCTCTCATGGAATGGCTAAAGACAAGACCAATTCTGTCACC26590.1166239323361892No Hit
GTGTTATCATTCCATTCAAGTCCTCCGATGAGGACCCCAATTGCATTTTT26480.11614147154051487No Hit
GGCAATGAGAGCCATTGGGACTCATCCTAGTTCCAGTACTGGTCTAAGAG26350.11557129060017245No Hit
CCTTTGACTAAGGGGATTTTAGGGTTTGTTTTCACGCTCACCGTGCCCAG26290.11530813016616827No Hit
GTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGACAAAATGACTG25970.11390460785147927No Hit
CTGTAGTGCTGGCCAAAACCATTCTGTTCTCATGTTTTATTAATGGATTG25750.1129396862601306No Hit
CCATTGGGACTCATCCTAGTTCCAGTACTGGTCTAAGAGATGATCTTCTT25650.11250108553679027No Hit
CTATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACT25510.11188704452411383No Hit
GACCAATTCTGTCACCTTTGACTAAGGGGATTTTAGGGTTTGTTTTCACG25440.1115800240177756No Hit
AGCTAAGGCTATGGAGCAAATGGCTGGATCAAGTGAGCAGGCAGCGGAGG25220.11061510242642693No Hit
GTGCAACATGTGAGCAGATTGCTGATTCCCAGCACAGGTCTCATAGGCAG24970.10951860061807614No Hit
CCCCCATTCGTTTCTGATAGGTCTGCAAATTTTCAAGAAGATCATCTCTT24950.10943088047340808No Hit
CCGTAGAAGGGCCTCTTTTAAGGCCGTGTTTGAATAGTCGATAGACGCAT24830.1089045596053997No Hit
CCTCAAAGCCGAGATCGCGCAGAGACTTGAAGATGTCTTTGCTGGGAAAA24770.10864139917139551No Hit
GCTAAGGCTATGGAGCAAATGGCTGGATCAAGTGAGCAGGCAGCGGAGGC24390.10697471642270233No Hit
GTGCAGGCAATGAGAGCCATTGGGACTCATCCTAGTTCCAGTACTGGTCT24330.10671155598869814No Hit
ATTGAAAGATGAGCCTTCTAACCGAGGTCGAAACGTATGTTCTCTCTATC24320.1066676959163641No Hit
TATGAGACCTGTGCTGGGAATCAGCAATCTGCTCACATGTTGCACACACC23950.10504487324000496No Hit
CAATGGGAATGGAGACCCAAATAACATGGACAAAGCAGTTAAACTGTATA23750.10416767179332433No Hit
CCTTTGGGGGACTCCCCAATGGGCCATGCTTCTGATTTATTCTCAAAAAA23470.10293958976797145No Hit
GCTTTGTCCATGTTATTTGGGTCTCCATTCCCATTGAGGGCATTTTGGAC23160.10157992752561648No Hit
GTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATCAGAAA23020.10096588651294003No Hit
CAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGTTCCTTTCGATAT22840.10017640521092748No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGCC100.0070777633145.09375145
AGCGAAA6350.0118.655381
TGCGCCC1100.0105.52272145
CGAAAGC7650.098.491713
GAGCGAA850.093.756541
AGTAGAA10750.084.916061
TCTCGGG608.258212E-1084.63802145
AGCGTAA1150.082.009514145
CGCGAAA406.643707E-572.546875145
CTTATAA502.2983459E-672.54687145
GGACAGT7350.071.960136
ATTATAT2100.068.9983141
TAAACTA1500.067.6198355
CAGTACG7950.067.444979
TAGAAAC14250.065.584723
TCTTACC451.19037126E-464.48611145
GACAGTA8450.064.3073967
ACAGTAC8400.063.8304448
GCAATCC1500.062.873955145
CGTATCC606.7823366E-660.45573145
Produced by FastQC (version 0.11.5)