FastQCFastQC Report
Tue 2 Jul 2019
HWC5JBCX2_l02_n02_SM2_9693_P2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l02_n02_SM2_9693_P2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1567621
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG160531.024035784159564No Hit
ATAATAAACAGTGAAGATGAGCATCTATTGGCACTTGAGAGAAAACTAAA91390.5829853006562173No Hit
GCTGTAGACATAGGAAACGGATGCTTCGAAACCAAACACAAATGCAACCA82140.5239786912780576No Hit
ATACTATACTGCTCTATTACTCAACTGCTGCTTCTAGTTTGGTTGTAACA77070.4916366902459204No Hit
CCTCAGAGCTGACACTATAAGCTCACAAATAGAACTTGCAGTCTTGCTTT76390.4872989070700125No Hit
CTATAAGCTCACAAATAGAACTTGCAGTCTTGCTTTCCAACGAAGGAATA73040.46592894583576006No Hit
ATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATGTT62510.39875709753824423No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC55790.3558895932116245No Hit
CCATATAAACAATAAAAATAGCTAGCATTAATGTTACAACCAAACTAGAA49100.3132134616721771No Hit
CTTATAGACAGATGGAGCATGAAACGTTGTCTCTGGAGACCATATAAACA46820.298669129847074No Hit
CAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGCAAACAAG46020.2935658555224764No Hit
CCTTTAATGCAGGAGAATTTTCTCTCCCCACTTTTGACTCATTGAACATT43810.2794680602007756No Hit
GCATTAATGTTACAACCAAACTAGAAGCAGCAGTTGAGTAATAGAGCAGT41550.26505131023378736No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT41160.26256346400054603No Hit
GTGAAGATGAGCATCTATTGGCACTTGAGAGAAAACTAAAGAAAATGCTG41110.26224450935525867No Hit
GAATAATAAACAGTGAAGATGAGCATCTATTGGCACTTGAGAGAAAACTA40590.25892738104427027No Hit
GATATACAGAAAGCACTAATTGGTGCCTCTATCTGCTTTTTAAAACCCAA40540.25860842639898296No Hit
GTAAGAAATACAGTAAAAATTGAATTTAATGCGATTCTTCTACCTCCAAA40500.25835326268275305No Hit
ATATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTCAT40400.2577153533921783No Hit
GAGTAATAGAGCAGTATAGTATGGTTATCCAATCCATCATCATTTAAAGA39230.2502518146924544No Hit
CTACAATAAAGGAAAATACAAGGCCTAACCTTCCTTATAGACAGATGGAG39130.24961390540187964No Hit
GTAATGTTCAATGAGTCAAAAGTGGGGAGAGAAAATTCTCCTGCATTAAA38650.24655194080712112No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC38040.24266069413461547No Hit
GTCTAAGCAGGTCTGGTTGCATTTGTGTTTGGTTTCGAAGCATCCGTTTC35940.22926459903254678No Hit
GCATTAAAGGTGCCAGCAGCTATCCTGTCTAAGCAGGTCTGGTTGCATTT35470.2262664253668457No Hit
CATTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAGCTCTGCTT34560.22046145082261592No Hit
AGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGCAAACAAGCA34430.2196321687448688No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA34350.21912184131240905No Hit
CCTTATAGACAGATGGAGCATGAAACGTTGTCTCTGGAGACCATATAAAC34120.21765464994408726No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG33210.2118496753998575No Hit
ATACAAGGCCTAACCTTCCTTATAGACAGATGGAGCATGAAACGTTGTCT32770.20904287452132883No Hit
TTACTGCTGCATCTTTAAATGATGATGGATTGGATAACCATACTATACTG32100.20476888227447834No Hit
CACTATAAGCTCACAAATAGAACTTGCAGTCTTGCTTTCCAACGAAGGAA31510.20100521746008762No Hit
CAAGAGGATTTTTCAATAACGTTTCTTTGTAATGATGACAAGCAAACAAG31360.20004835352422554No Hit
GTGTGAATGTGATGCTTGTTTTTCGCACAAAGCACAGAGTGTTCCTAGTT30730.19602952499360496No Hit
CAAATAGAACTTGCAGTCTTGCTTTCCAACGAAGGAATAATAAACAGTGA30510.19462612455434064No Hit
GCTTAGACAGGATAGCTGCTGGCACCTTTAATGCAGGAGAATTTTCTCTC30220.192776187611674No Hit
GAACATTACTGCTGCATCTTTAAATGATGATGGATTGGATAACCATACTA29640.18907631372634073No Hit
TCCTTATAGACAGATGGAGCATGAAACGTTGTCTCTGGAGACCATATAAA29570.18862977722293844No Hit
GGATAGCTGCTGGCACCTTTAATGCAGGAGAATTTTCTCTCCCCACTTTT29550.18850219536482352No Hit
GAGTGTTCCTAGTTTTACTTGCATTGAATAATTTCCAGGATTCAGGTACA28600.18244205710436387No Hit
CACGTGCTGGATGAAAAAGTGGATGACCTCAGAGCTGACACTATAAGCTC28450.18148519316850184No Hit
GGAAAATACAAGGCCTAACCTTCCTTATAGACAGATGGAGCATGAAACGT28350.18084728387792712No Hit
GAGCAGTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAG28280.18040074737452483No Hit
GCTTTAGCACTTCCATTACATCCTTTGCAATTCCTTCCCCATTTTTCTGA28120.17938009250960532No Hit
CTACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAAT26730.170513153370617No Hit
CAATAAAGGAAAATACAAGGCCTAACCTTCCTTATAGACAGATGGAGCAT25990.16579262462036423No Hit
CAGTGAAGATGAGCATCTATTGGCACTTGAGAGAAAACTAAAGAAAATGC25680.16381510581958267No Hit
GTTCATAGCTGAGACCATCTGCATTTCCCGTCTCACTCCTGGCACTGAGG25310.1614548414444563No Hit
GCAGTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTA25270.1611996777282264No Hit
GCCTTGGAATGGATAAAAAACAAAAGATGCTTAACTGATATACAGAAAGC25240.161008304941054No Hit
GTCTACAGCAGAGGGACCCAGCATTTTCTTTAGTTTTCTCTCAAGTGCCA24840.1584566677787552No Hit
TGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATGTTCAATGA24640.1571808491976058No Hit
CTTTAATGCAGGAGAATTTTCTCTCCCCACTTTTGACTCATTGAACATTA24180.15424646646096218No Hit
CTTTTCATACAGTATGGCTCAAACCCTTCAATTCCAGCAGATGGATTCAT23910.1525241113764105No Hit
CACGAGCTGGATGAAAAAGTGGATGACCTCAGAGCTGACACTATAAGCTC23460.14965351956882436No Hit
GTAGAAACACGAGCTGGATGAAAAAGTGGATGACCTCAGAGCTGACACTA23440.1495259377107094No Hit
GAATAAAGGGGCCAAATAAAGAGACAATAAACAGAGAGGTATCAATTTTG23300.14863286470390483No Hit
CATTCACACAGAGCTCATAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGT22980.14659155497406579No Hit
CTATTGGCACTTGAGAGAAAACTAAAGAAAATGCTGGGTCCCTCTGCTGT22740.1450605726766865No Hit
ACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGC22730.14499678174762906No Hit
GTCCCTCTGCTGTAGACATAGGAAACGGATGCTTCGAAACCAAACACAAA22660.14455024524422677No Hit
GCAGTAATGTTCAATGAGTCAAAAGTGGGGAGAGAAAATTCTCCTGCATT22180.14148828064946822No Hit
GTCCTATTGTCCAAGCCATGAAATGGAGAGCTGATAAAATAAAAGAACAA22080.1408503713588935No Hit
CCATACTATACTGCTCTATTACTCAACTGCTGCTTCTAGTTTGGTTGTAA22030.14053141671360617No Hit
TAATAGAGCAGTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGC21930.13989350742303147No Hit
GCTATGAACACAGCAAAAACAATGAATGGAATGGGGAAAGGAGAAGACGT21410.13657637911204304No Hit
ATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAG21290.1358108879633534No Hit
CTTTTGACTCATTGAACATTACTGCTGCATCTTTAAATGATGATGGATTG21130.13479023309843385No Hit
CAATAAAAATAGCTAGCATTAATGTTACAACCAAACTAGAAGCAGCAGTT21080.13447127845314652No Hit
CACAAATAGAACTTGCAGTCTTGCTTTCCAACGAAGGAATAATAAACAGT20890.1332592508010546No Hit
GGATGAAAAAGTGGATGACCTCAGAGCTGACACTATAAGCTCACAAATAG20640.13166447757461786No Hit
GGCCTAACCTTCCTTATAGACAGATGGAGCATGAAACGTTGTCTCTGGAG20210.12892146762514664No Hit
AGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACATTGATTACAAT20210.12892146762514664No Hit
TCTTTGTAATGATGACAAGCAAACAAGCACTACAATAAAGGAAAATACAA20050.12790081276022713No Hit
TGCTTAGACAGGATAGCTGCTGGCACCTTTAATGCAGGAGAATTTTCTCT20010.12764564904399725No Hit
GCTATGAGCTCTGTGTGAATGTGATGCTTGTTTTTCGCACAAAGCACAGA20010.12764564904399725No Hit
ATACTGCTCTATTACTCAACTGCTGCTTCTAGTTTGGTTGTAACATTAAT19980.12745427625682484No Hit
AGCAGGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT19910.12700773975342255No Hit
CAGCAGTAATGTTCAATGAGTCAAAAGTGGGGAGAGAAAATTCTCCTGCA19820.1264336213919053No Hit
CTGCTGTAGACATAGGAAACGGATGCTTCGAAACCAAACACAAATGCAAC19470.12420093887489388No Hit
ATACTATACTGCTCTATTACTCAACTGCTGCTTCTAGTTTGGCTGTAACA19090.12177688357071001No Hit
CATTAATGCTAGCTATTTTTATTGTTTATATGGTCTCCAGAGACAACGTT18980.12107518335107784No Hit
AACAACAGCTGGATGAAAAAGTGGATGACCTCAGAGCTGACACTATAAGC18760.11967178291181349No Hit
AACACGAGCTGGATGAAAAAGTGGATGACCTCAGAGCTGACACTATAAGC18730.1194804101246411No Hit
GTTGAGTAATAGAGCAGTATAGTATGGTTATCCAATCCATCATCATTTAA18600.11865112804689398No Hit
GTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATGTTCAA18510.11807700968537677No Hit
CATATAAACAATAAAAATAGCTAGCATTAATGTTACAACCAAACTAGAAG18400.11737530946574459No Hit
GCACAGAGTGTTCCTAGTTTTACTTGCATTGAATAATTTCCAGGATTCAG18300.1167374001751699No Hit
ATCCAATCCATCATCATTTAAAGATGCAGCAGTAATGTTCAATGAGTCAA17890.11412197208381364No Hit
GCCTAACCTTCCTTATAGACAGATGGAGCATGAAACGTTGTCTCTGGAGA17720.11303752628983664No Hit
CTATTACTCAACTGCTGCTTCTAGTTTGGTTGTAACATTAATGCTAGCTA17660.11265478071549181No Hit
GTATAAATACTGGGACAATTGACAAAACACCGGAAGAAATAACTTCTGGA17640.11252719885737689No Hit
AATAAACAGTGAAGATGAGCATCTATTGGCACTTGAGAGAAAACTAAAGA17320.11048588912753785No Hit
ATTACATCCTTTGCAATTCCTTCCCCATTTTTCTGACTTGCCCCAAGGGA17170.10952902519167579No Hit
GTATGGCTCAAACCCTTCAATTCCAGCAGATGGATTCATAAATAAAAGTA17170.10952902519167579No Hit
GGTCATATGCATTCAATCTATGCAGAGTTGATAAGGACTTGTATCCATTG17090.10901869775921604No Hit
AACAAGCACTACAATAAAGGAAAATACAAGGCCTAACCTTCCTTATAGAC16880.10767908824900917No Hit
GGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATGTTCAATGAG16840.10742392453277928No Hit
ACAACAGCTGGATGAAAAAGTGGATGACCTCAGAGCTGACACTATAAGCT16830.10736013360372182No Hit
GGATAACCATACTATACTGCTCTATTACTCAACTGCTGCTTCTAGTTTGG16770.106977388029377No Hit
GGTCAAGACCGTCTAAACAGACTAAAGAGGAAATTAGAGTCAAGAATAAA16670.10633947873880228No Hit
CTCTTGAAGAGAGAAAAGCAATTGGAGTAAAAATGATGAAAGTACTTTTA16630.10608431502257241No Hit
GTCATGTACCTGAATCCTGGAAATTATTCAATGCAAGTAAAACTAGGAAC16590.10582915130634253No Hit
CTCCAATGTTGCTTTGCAGTTCTTCTGCCAGTTTTTGGACGTCTTCTCCT16550.10557398759011266No Hit
GTCTTGCTTTCCAACGAAGGAATAATAAACAGTGAAGATGAGCATCTATT16300.10397921436367591No Hit
TCTATTGGCACTTGAGAGAAAACTAAAGAAAATGCTGGGTCCCTCTGCTG16220.10346888693121616No Hit
ATCCATCATCATTTAAAGATGCAGCAGTAATGTTCAATGAGTCAAAAGTG16200.10334130507310123No Hit
TCATAATCCTCTGCTGTGTCCCTCCCAAAGAAGAAATTGGGGATGGTCTG16190.10327751414404374No Hit
CTTCGAAACCAAACACAAATGCAACCAGACCTGCTTAGACAGGATAGCTG15900.10142757720137711No Hit
CTATTGTCCAAGCCATGAAATGGAGAGCTGATAAAATAAAAGAACAAATT15880.10129999534326219No Hit
TAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATGTTC15880.10129999534326219No Hit
GTATGAAAAGTTCCTCAGAGAGCAACTGTCCGAAATACAGTTGGATTGAT15870.10123620441420472No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCCC151.2264539E-4145.1092145
CGAGCGG202.123019E-6145.10919145
ATACGCC100.0070753145.10919145
GGCACCC3250.0142.87675145
AGCACGC5950.0110.781811
AGCCACC203.854908E-4108.8319145
CACGCAC6200.0107.486533
GCACGCA6200.0107.48312
GCCGACC607.2759576E-1296.73946145
TGACGCC1250.092.869896145
CGACGGG554.1472958E-1092.34222145
GCGGTGC554.2018655E-1092.1890641
CGGTGCA406.1522405E-790.542832
GCGGAGC651.6370905E-1189.1498641
TAGTAAC25900.083.621022
GTGGGGG702.910383E-1182.91954145
GTCCTGG353.45339E-582.782011
CAGCGCC451.2277051E-680.61622145
TCTCGGG1200.078.600815145
AAACGGG651.5552359E-978.13573145
Produced by FastQC (version 0.11.5)