FastQCFastQC Report
Thu 11 Jul 2019
HWC5JBCX2_l02_n02_SM2_9693_P2_DM2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l02_n02_SM2_9693_P2_DM2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1575466
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG161081.0224276499778477No Hit
ATAATAAACAGTGAAGATGAGCATCTATTGGCACTTGAGAGAAAACTAAA91640.5816691696298111No Hit
GCTGTAGACATAGGAAACGGATGCTTCGAAACCAAACACAAATGCAACCA82450.5233372221298332No Hit
ATACTATACTGCTCTATTACTCAACTGCTGCTTCTAGTTTGGTTGTAACA77300.49064848114780013No Hit
CCTCAGAGCTGACACTATAAGCTCACAAATAGAACTTGCAGTCTTGCTTT76580.48607840473866143No Hit
CTATAAGCTCACAAATAGAACTTGCAGTCTTGCTTTCCAACGAAGGAATA73240.46487832806293505No Hit
ATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATGTT62660.3977235941619812No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC55950.35513302096014765No Hit
CCATATAAACAATAAAAATAGCTAGCATTAATGTTACAACCAAACTAGAA49260.3126693943252346No Hit
CTTATAGACAGATGGAGCATGAAACGTTGTCTCTGGAGACCATATAAACA46920.29781664599553404No Hit
CAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGCAAACAAG46120.2927387833187133No Hit
CCTTTAATGCAGGAGAATTTTCTCTCCCCACTTTTGACTCATTGAACATT43960.2790285540912974No Hit
GCATTAATGTTACAACCAAACTAGAAGCAGCAGTTGAGTAATAGAGCAGT41670.2644931721788982No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT41320.26227160725778914No Hit
GTGAAGATGAGCATCTATTGGCACTTGAGAGAAAACTAAAGAAAATGCTG41270.26195424084048785No Hit
GAATAATAAACAGTGAAGATGAGCATCTATTGGCACTTGAGAGAAAACTA40760.25871710338401466No Hit
GTAAGAAATACAGTAAAAATTGAATTTAATGCGATTCTTCTACCTCCAAA40700.2583362636832531No Hit
GATATACAGAAAGCACTAATTGGTGCCTCTATCTGCTTTTTAAAACCCAA40630.25789195069903126No Hit
ATATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTCAT40580.25757458428172997No Hit
GAGTAATAGAGCAGTATAGTATGGTTATCCAATCCATCATCATTTAAAGA39380.24995779026649895No Hit
CTACAATAAAGGAAAATACAAGGCCTAACCTTCCTTATAGACAGATGGAG39270.24925958414843608No Hit
GTAATGTTCAATGAGTCAAAAGTGGGGAGAGAAAATTCTCCTGCATTAAA38760.24602244669196288No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC38100.2418332099835858No Hit
GTCTAAGCAGGTCTGGTTGCATTTGTGTTTGGTTTCGAAGCATCCGTTTC36090.2290750800080738No Hit
GCATTAAAGGTGCCAGCAGCTATCCTGTCTAAGCAGGTCTGGTTGCATTT35530.22552057613429932No Hit
CATTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAGCTCTGCTT34610.2196810340559555No Hit
AGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGCAAACAAGCA34610.2196810340559555No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA34420.2184750416702106No Hit
CCTTATAGACAGATGGAGCATGAAACGTTGTCTCTGGAGACCATATAAAC34190.21701515615062464No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG33300.21136603392266162No Hit
ATACAAGGCCTAACCTTCCTTATAGACAGATGGAGCATGAAACGTTGTCT32880.20870015601733072No Hit
TTACTGCTGCATCTTTAAATGATGATGGATTGGATAACCATACTATACTG32190.20432049945857286No Hit
CACTATAAGCTCACAAATAGAACTTGCAGTCTTGCTTTCCAACGAAGGAA31610.20063904901787788No Hit
CAAGAGGATTTTTCAATAACGTTTCTTTGTAATGATGACAAGCAAACAAG31430.19949652991559322No Hit
GTGTGAATGTGATGCTTGTTTTTCGCACAAAGCACAGAGTGTTCCTAGTT30820.19562465962451744No Hit
CAAATAGAACTTGCAGTCTTGCTTTCCAACGAAGGAATAATAAACAGTGA30610.19429172067185202No Hit
GCTTAGACAGGATAGCTGCTGGCACCTTTAATGCAGGAGAATTTTCTCTC30340.19257794201842504No Hit
GAACATTACTGCTGCATCTTTAAATGATGATGGATTGGATAACCATACTA29750.18883301829426977No Hit
TCCTTATAGACAGATGGAGCATGAAACGTTGTCTCTGGAGACCATATAAA29700.18851565187696845No Hit
GGATAGCTGCTGGCACCTTTAATGCAGGAGAATTTTCTCTCCCCACTTTT29590.1878174457589056No Hit
GAGTGTTCCTAGTTTTACTTGCATTGAATAATTTCCAGGATTCAGGTACA28700.1821683235309426No Hit
CACGTGCTGGATGAAAAAGTGGATGACCTCAGAGCTGACACTATAAGCTC28530.1810892777121182No Hit
GGAAAATACAAGGCCTAACCTTCCTTATAGACAGATGGAGCATGAAACGT28510.1809623311451977No Hit
GAGCAGTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAG28330.17981981204291303No Hit
GCTTTAGCACTTCCATTACATCCTTTGCAATTCCTTCCCCATTTTTCTGA28190.1789311860744694No Hit
CTACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAAT26770.16991797982311266No Hit
CAATAAAGGAAAATACAAGGCCTAACCTTCCTTATAGACAGATGGAGCAT26090.16560179654781507No Hit
CAGTGAAGATGAGCATCTATTGGCACTTGAGAGAAAACTAAAGAAAATGC25730.16331675834324574No Hit
GTTCATAGCTGAGACCATCTGCATTTCCCGTCTCACTCCTGGCACTGAGG25400.1612221399890572No Hit
GCAGTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTA25340.16084130028829566No Hit
GCCTTGGAATGGATAAAAAACAAAAGATGCTTAACTGATATACAGAAAGC25300.1605874071544546No Hit
GTCTACAGCAGAGGGACCCAGCATTTTCTTTAGTTTTCTCTCAAGTGCCA24910.15811194909950455No Hit
TGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATGTTCAATGA24730.15696942999721988No Hit
CTTTAATGCAGGAGAATTTTCTCTCCCCACTTTTGACTCATTGAACATTA24240.1538592391076672No Hit
CTTTTCATACAGTATGGCTCAAACCCTTCAATTCCAGCAGATGGATTCAT23970.1521454604542402No Hit
GTAGAAACACGAGCTGGATGAAAAAGTGGATGACCTCAGAGCTGACACTA23520.14928916269852857No Hit
CACGAGCTGGATGAAAAAGTGGATGACCTCAGAGCTGACACTATAAGCTC23480.14903526956468752No Hit
GAATAAAGGGGCCAAATAAAGAGACAATAAACAGAGAGGTATCAATTTTG23350.14821011687970417No Hit
CATTCACACAGAGCTCATAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGT23030.14617897180897588No Hit
CTATTGGCACTTGAGAGAAAACTAAAGAAAATGCTGGGTCCCTCTGCTGT22850.14503645270669122No Hit
ACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGC22790.1446556130059297No Hit
GTCCCTCTGCTGTAGACATAGGAAACGGATGCTTCGAAACCAAACACAAA22750.14440171987208864No Hit
GCAGTAATGTTCAATGAGTCAAAAGTGGGGAGAGAAAATTCTCCTGCATT22250.1412280556990757No Hit
GTCCTATTGTCCAAGCCATGAAATGGAGAGCTGATAAAATAAAAGAACAA22130.1404663762975526No Hit
CCATACTATACTGCTCTATTACTCAACTGCTGCTTCTAGTTTGGTTGTAA22110.14033942973063207No Hit
TAATAGAGCAGTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGC22010.1397046968960295No Hit
GCTATGAACACAGCAAAAACAATGAATGGAATGGGGAAAGGAGAAGACGT21470.1362771395891755No Hit
ATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAG21350.13551546018765243No Hit
CTTTTGACTCATTGAACATTACTGCTGCATCTTTAAATGATGATGGATTG21190.13449988765228826No Hit
CAATAAAAATAGCTAGCATTAATGTTACAACCAAACTAGAAGCAGCAGTT21170.13437294108536776No Hit
CACAAATAGAACTTGCAGTCTTGCTTTCCAACGAAGGAATAATAAACAGT20930.13284958228232155No Hit
GGATGAAAAAGTGGATGACCTCAGAGCTGACACTATAAGCTCACAAATAG20670.1311992769123548No Hit
GGCCTAACCTTCCTTATAGACAGATGGAGCATGAAACGTTGTCTCTGGAG20270.12866034557394448No Hit
AGTAGAAACAACGCACTTTTTCCAGTTTATTTGCTGACATTGATTACAAT20270.12866034557394448No Hit
TCTTTGTAATGATGACAAGCAAACAAGCACTACAATAAAGGAAAATACAA20110.12764477303858032No Hit
ATACTGCTCTATTACTCAACTGCTGCTTCTAGTTTGGTTGTAACATTAAT20070.1273908799047393No Hit
TGCTTAGACAGGATAGCTGCTGGCACCTTTAATGCAGGAGAATTTTCTCT20060.12732740662127906No Hit
GCTATGAGCTCTGTGTGAATGTGATGCTTGTTTTTCGCACAAAGCACAGA20050.12726393333781877No Hit
AGCAGGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT20000.12694656692051748No Hit
CAGCAGTAATGTTCAATGAGTCAAAAGTGGGGAGAGAAAATTCTCCTGCA19870.1261214142355341No Hit
CTGCTGTAGACATAGGAAACGGATGCTTCGAAACCAAACACAAATGCAAC19530.12396332259788533No Hit
ATACTATACTGCTCTATTACTCAACTGCTGCTTCTAGTTTGGCTGTAACA19100.1212339714090942No Hit
CATTAATGCTAGCTATTTTTATTGTTTATATGGTCTCCAGAGACAACGTT19060.12098007827525317No Hit
AACAACAGCTGGATGAAAAAGTGGATGACCTCAGAGCTGACACTATAAGC18870.11977408588950825No Hit
AACACGAGCTGGATGAAAAAGTGGATGACCTCAGAGCTGACACTATAAGC18790.11926629962182617No Hit
GTTGAGTAATAGAGCAGTATAGTATGGTTATCCAATCCATCATCATTTAA18640.11831420036992228No Hit
GTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATGTTCAA18590.117996833952621No Hit
CATATAAACAATAAAAATAGCTAGCATTAATGTTACAACCAAACTAGAAG18450.11710820798417737No Hit
GCACAGAGTGTTCCTAGTTTTACTTGCATTGAATAATTTCCAGGATTCAG18380.11666389499995555No Hit
ATCCAATCCATCATCATTTAAAGATGCAGCAGTAATGTTCAATGAGTCAA17970.11406149037808495No Hit
GCCTAACCTTCCTTATAGACAGATGGAGCATGAAACGTTGTCTCTGGAGA17800.11298244455926056No Hit
GTATAAATACTGGGACAATTGACAAAACACCGGAAGAAATAACTTCTGGA17720.11247465829157849No Hit
CTATTACTCAACTGCTGCTTCTAGTTTGGTTGTAACATTAATGCTAGCTA17710.11241118500811824No Hit
AATAAACAGTGAAGATGAGCATCTATTGGCACTTGAGAGAAAACTAAAGA17410.11050698650431046No Hit
GTATGGCTCAAACCCTTCAATTCCAGCAGATGGATTCATAAATAAAAGTA17280.1096818338193271No Hit
ATTACATCCTTTGCAATTCCTTCCCCATTTTTCTGACTTGCCCCAAGGGA17270.10961836053586685No Hit
GGTCATATGCATTCAATCTATGCAGAGTTGATAAGGACTTGTATCCATTG17130.10872973456742321No Hit
AACAAGCACTACAATAAAGGAAAATACAAGGCCTAACCTTCCTTATAGAC16920.10739679561475779No Hit
ACAACAGCTGGATGAAAAAGTGGATGACCTCAGAGCTGACACTATAAGCT16870.10707942919745649No Hit
GGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATGTTCAATGAG16870.10707942919745649No Hit
GGATAACCATACTATACTGCTCTATTACTCAACTGCTGCTTCTAGTTTGG16810.10669858949669495No Hit
GGTCAAGACCGTCTAAACAGACTAAAGAGGAAATTAGAGTCAAGAATAAA16730.10619080322901288No Hit
CTCTTGAAGAGAGAAAAGCAATTGGAGTAAAAATGATGAAAGTACTTTTA16670.10580996352825132No Hit
GTCATGTACCTGAATCCTGGAAATTATTCAATGCAAGTAAAACTAGGAAC16620.10549259711095003No Hit
CTCCAATGTTGCTTTGCAGTTCTTCTGCCAGTTTTTGGACGTCTTCTCCT16620.10549259711095003No Hit
GTCTTGCTTTCCAACGAAGGAATAATAAACAGTGAAGATGAGCATCTATT16340.10371534517406278No Hit
TCTATTGGCACTTGAGAGAAAACTAAAGAAAATGCTGGGTCCCTCTGCTG16270.10327103218984096No Hit
ATCCATCATCATTTAAAGATGCAGCAGTAATGTTCAATGAGTCAAAAGTG16260.10320755890638073No Hit
TCATAATCCTCTGCTGTGTCCCTCCCAAAGAAGAAATTGGGGATGGTCTG16200.10282671920561917No Hit
GTATGAAAAGTTCCTCAGAGAGCAACTGTCCGAAATACAGTTGGATTGAT15950.10123988711911269No Hit
CTATTGTCCAAGCCATGAAATGGAGAGCTGATAAAATAAAAGAACAAATT15950.10123988711911269No Hit
CTTCGAAACCAAACACAAATGCAACCAGACCTGCTTAGACAGGATAGCTG15940.10117641383565244No Hit
TAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATGTTC15900.10092252070181139No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCGAA100.007078509145.08722145
GCTAGCC151.2271953E-4145.08722145
GGCACCC3350.0134.25981145
CGCACCC351.895387E-9124.36048145
AGCACGC5800.0116.142721
CACGCAC5900.0115.4055563
GCACGCA5950.0114.432122
TGACGCC404.796675E-9108.815414145
GTCCTGG650.0100.2919851
CGGTGCA950.099.118982
GCGGTGC1100.098.772411
GCCGACC554.1472958E-1092.328224145
AAACGGG502.2600943E-887.05234145
CCGACGC259.3653554E-487.05234145
GACGACC451.2288147E-680.60401145
TAGTAAC24500.079.528592
GGGTATA1050.075.882291
GGTATAC1050.075.882292
CGACGGG406.64455E-572.54361145
TTGTCGA300.001943052972.43311
Produced by FastQC (version 0.11.5)