FastQCFastQC Report
Tue 2 Jul 2019
HWC5JBCX2_l02_n02_SM2_9609_P2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l02_n02_SM2_9609_P2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1103495
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTGTGCGTGTGTGGAAGATGGAACGGGATGGCAAACAGTGACATCACC76760.6956080453468299No Hit
AAGCAGGGCAGTGGGCAGTGTGTGCGTGTGTGGAAGATGGAACGGGATGG45210.40969827683859006No Hit
GGGTGAGGTTGGCAGGGTGAGGTTGGCAGGGTGAGGCTGGCAGGGTGAGG41970.380337020104305No Hit
GCAGTGGGCAGTGTGTGCGTGTGTGGAAGATGGAACGGGATGGCAAACAG36170.32777674570342413No Hit
GTGTGCGTGTGTGGAAGATGGAACGGGATGGCAAACAGTGACATCACCAA32340.2930688403662907No Hit
AGCGAAAGCAGGGCAGTGGGCAGTGTGTGCGTGTGTGGAAGATGGAACGG29750.26959795921141466No Hit
CATATATACAGTATGTTACCAAGACACAGCAAAAAGATAACTATGCTACA29680.26896361107209366No Hit
AGCGAAAGCAGGGTGAGGTTGGCAGGGTGAGGTTGGCAGGGTGAGGCTGG27920.25301428642630913No Hit
CATTATATGTGTGCTTTTGGGGTGTAGATGTGAGGACATAGTCAGATCTT27190.24639894154481895No Hit
GCATTATGCCATGGAGGTTCAGATTACGCTCGTCCACTCCTGCTTGCACT25240.2287278148065918No Hit
GTGTGTGTGTGTGTGACAGGGTGAGGCTGGAGGCTCCTCTGACTGTTCTT24670.22356240852926382No Hit
GTATACACATATTGTGTAATGTGTATGTGTGTTATGTTTCTGTCTGGAGG23840.21604085202017229No Hit
GGGCAGTGTGTGCGTGTGTGGAAGATGGAACGGGATGGCAAACAGTGACA23510.21305035364908767No Hit
CTCTTGTGGTGTTTATGCAAAAGCCGGTCACGTGAAGTCTGCAAATGTAC21790.1974635136543437No Hit
GGTTGGCAGGGTGAGGTTGGCAGGGTGAGGCTGGCAGGGTGAGGTTGGCA21390.1938386671439381No Hit
CATTTAAGGAGCGCAGAGCTGAGAGTCAGCCGGGCGTCTGATTGGTGGCG21370.19365742481841786No Hit
AAAGCAGGGTGAGGTTGGCAGGGTGAGGTTGGCAGGGTGAGGCTGGCAGG20630.18695145877416752No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT19660.178161205986434No Hit
CATATAATGCATACATATGCATTGTAATCATTTTGTGTTAAAAAAAAGAC19280.17471760180154872No Hit
CATAAAACAGCGTGCCGGGTCCCAAACAGACGGAACATTCATTTAGGTTT19200.17399263249946761No Hit
ATATAGAACAGCAAACGCAATTACCCCATCAACAAGTTAAGCTGTTGAAA18600.16855536273385924No Hit
ATTATGCACCGTTGAAAAAAGGCCCAGGTTTTGCCCAGCTGCCTCACTGC18430.16701480296693688No Hit
GCAGGGCAGTGGGCAGTGTGTGCGTGTGTGGAAGATGGAACGGGATGGCA18290.1657461066882949No Hit
GGGCAGTGGGCAGTGTGTGCGTGTGTGGAAGATGGAACGGGATGGCAAAC18230.16520237971173407No Hit
GGGTGAGGCTGGCAGGGTGAGGTTGGCAGGGTGAGGCTGGCAGGGTGAGG18060.16366181994481172No Hit
GATTTATATTTTATTGATCATTTCACCTGTGAATAAATTATGCACCGTTG17860.1618493966896089No Hit
AGGCTGGAGTGTGTGTGTGTGTGTGACAGGGTGAGGCTGGAGGCTCCTCT17560.15913076180680474No Hit
GGGTGAGGTTGGCAGGGTGAGGCTGGCAGGGTGAGGTTGGCAGGGTGAGG17490.15849641366748377No Hit
GTGTGTGACAGGGTGAGGCTGGAGGCTCCTCTGACTGTTCTTTACAATGA16400.14861870692662857No Hit
GGGTGTACAGTCAGGAGGAACAATGCCATCTGCGTGGGGGCCATTCACCG16370.14834684343834817No Hit
GCATAATGCACCCCTGCAGAAGCCCCCTGCATTTGCGTAAGCAGCCACAT16360.14825622227558802No Hit
GTGTGTGTGTGTGTGCCTGTGTGTGTCAAGGGAAGAGGGGGGAAGGGTGT15610.14145963506857756No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACA15340.1390128636740538No Hit
GTCCAGGAGGTCTGTGCTCCAGGAGGTCTGTGCTCCATCTTCACGGAGGA15000.1359317441402091No Hit
ATGCATATGTATGCATTATATGTGTGCTTTTGGGGTGTAGATGTGAGGAC14810.13420994204776643No Hit
ATACAGGGAAGAGGATCCTGTCAACCTACGAAGATCACTCCCTCCCTCTC14470.1311288225139217No Hit
GTTTTAACAACGCTCACAACACAGGGAGATCAGATCAGATCAGAACGTGC14350.1300413685608No Hit
GTTCTTTACAATGAGAGGGAGGGAGTGATCTTCGTAGGTTGACAGGATCC14290.12949764158423918No Hit
GTATGTATGTGTGTACCTGAGCTTGCTCTTCAAGGTGTTCACTTCGCGGT14130.12804770298007695No Hit
GTGGGCAGTGTGTGCGTGTGTGGAAGATGGAACGGGATGGCAAACAGTGA14130.12804770298007695No Hit
GTGTGTGTGTGTGTGTGCCTGTGTGTGTCAAGGGAAGAGGGGGGAAGGGT13970.12659776437591472No Hit
GGGTGAGGTTGGCAGGGTGAGGCTGGCAGGGTGAGGCTGGAGTGTGTGTG13600.12324478135378955No Hit
GTGTGTGTGTGTGTGTGACAGGGTGAGGCTGGAGGCTCCTCTGACTGTTC13560.12288229670274899No Hit
CTTCAGAGTCACCTCGGTTTGGGATTACAAATCTTTGACCAAGGACCACA13370.12116049461030635No Hit
GCATATGTATGCATTATATGTGTGCTTTTGGGGTGTAGATGTGAGGACAT13000.1178075115881812No Hit
GTGTGTGTGTGTGACAGGGTGAGGCTGGAGGCTCCTCTGACTGTTCTTTA12930.11717316344886021No Hit
TATATACAGTATGTTACCAAGACACAGCAAAAAGATAACTATGCTACACG12900.1169012999605798No Hit
CCAAATCAACTGTCCGAGCTGCCACCATGTCAGCCGATCACAATACAGGG12850.11644819414677911No Hit
CTGTAGGCAAGCATAAGTGACTTGAACTTAATGCGAGCAGCTACCGGCAG12850.11644819414677911No Hit
TCACAATACAGGGAAGAGGATCCTGTCAACCTACGAAGATCACTCCCTCC12690.11499825554261688No Hit
GGTTGGCAGGGTGAGGCTGGCAGGGTGAGGTTGGCAGGGTGAGGCTGGCA12670.11481701321709659No Hit
CCTCTGACTGTTCTTTACAATGAGAGGGAGGGAGTGATCTTCGTAGGTTG12590.11409204391501547No Hit
CTCTTGTGGTGTTTAGGCAAAAGCCGGTCACGTGAAGTCTGCAAATGTAC12400.11237024182257283No Hit
CAATACAGGGAAGAGGATCCTGTCAACCTACGAAGATCACTCCCTCCCTC12350.11191713600877214No Hit
GAGTTATATCATAGTCTTCATGAACATACCCGAAGCTCCTTTGTTATTAT12310.11155465135773157No Hit
GAGGTATACTTTGTAGTAAAACAAAGTAATCTGTGTGTGTGTGTGTGTGT12020.10892663763768753No Hit
GTGTGTGTGTGTGCCTGTGTGTGTCAAGGGAAGAGGGGGGAAGGGTGTTA11790.10684235089420432No Hit
GGATGGCAAACAGTGACATCACCAAATCAACTGTCCGAGCTGCCACCATG11740.10638924508040364No Hit
GGGCTAATCCTGTGCATGCAGCACGACACGAAGAACACCGACAAACTGGA11720.10620800275488335No Hit
ATCGAAACCTGTGTCCCCGCGGGCATTGTAGCCCGTAAATGGGGGGCTTA11710.10611738159212322No Hit
AAGCAGGGCAGCCTTGCGGAGACAGGTGGTGACACTCGAGGTCCACTCTG11640.10548303345280223No Hit
GTCCAATATCAGGCTGTATACTGTTATTGTATTTGCTACAGTTATATGCC11610.10521116996452182No Hit
GTATACTGTTATTGTATTTGCTACAGTTATATGCCATTATGAGCTTGTCG11590.10502992763900154No Hit
GGGTGAGGCTGGAGGCTCCTCTGACTGTTCTTTACAATGAGAGGGAGGGA11590.10502992763900154No Hit
AGCGAAAGCAGGGCAGCCTTGCGGAGACAGGTGGTGACACTCGAGGTCCA11520.10439557949968056No Hit
CATTATAAGAGGTATACTTTGTAGTAAAACAAAGTAATCTGTGTGTGTGT11320.10258315624447777No Hit
GTCTGTGATAATACATCACAGGCGGCACATTGTATATCAGAAATCAATGA11300.10240191391895749No Hit
GTGGAAGATGGAACGGGATGGCAAACAGTGACATCACCAAATCAACTGTC11240.10185818694239666No Hit
GGGTGAGGCTGGCAGGGTGAGGCTGGAGTGTGTGTGTGTGTGTGACAGGG11220.10167694461687639No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTCGGG100.007077343145.09326145
AGCGAAA20600.0120.975651
GATCACC2950.0115.582756145
GCGAAAG26200.095.394772
CGAAAGC28700.087.085123
GATACAA259.3630597E-487.055954145
ATAGCCC353.4264893E-582.91042145
GAGCAAA3200.079.236411
CCTTACC554.375397E-879.14177145
TGGTTCC554.375397E-879.14177145
TACTGGG1500.077.2848747
AAGCGCC300.00193086472.54662145
CCCGGCC554.0464092E-665.95148145
GACATAA1100.065.858831
TTGCCCC808.047209E-963.4783145
ATAAGTA1150.062.9954073
GTCAAGA350.003577395862.0954671
TATAAGT1251.8189894E-1257.955772
CGACGAA2550.056.89932145
TAGAAAC1206.002665E-1154.3335382
Produced by FastQC (version 0.11.5)