FastQCFastQC Report
Thu 11 Jul 2019
HWC5JBCX2_l02_n02_SM2_9609_P2_DM2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l02_n02_SM2_9609_P2_DM2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1111201
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTGTGCGTGTGTGGAAGATGGAACGGGATGGCAAACAGTGACATCACC77150.6942938316290213No Hit
AAGCAGGGCAGTGGGCAGTGTGTGCGTGTGTGGAAGATGGAACGGGATGG45410.4086569396535821No Hit
GGGTGAGGTTGGCAGGGTGAGGTTGGCAGGGTGAGGCTGGCAGGGTGAGG42170.3794992985067508No Hit
GCAGTGGGCAGTGTGTGCGTGTGTGGAAGATGGAACGGGATGGCAAACAG36380.3273935138647283No Hit
GTGTGCGTGTGTGGAAGATGGAACGGGATGGCAAACAGTGACATCACCAA32540.292836309542558No Hit
AGCGAAAGCAGGGCAGTGGGCAGTGTGTGCGTGTGTGGAAGATGGAACGG29900.2690782315710659No Hit
CATATATACAGTATGTTACCAAGACACAGCAAAAAGATAACTATGCTACA29810.268268297094765No Hit
AGCGAAAGCAGGGTGAGGTTGGCAGGGTGAGGTTGGCAGGGTGAGGCTGG28040.25233958572751464No Hit
CATTATATGTGTGCTTTTGGGGTGTAGATGTGAGGACATAGTCAGATCTT27290.24559013175834074No Hit
GCATTATGCCATGGAGGTTCAGATTACGCTCGTCCACTCCTGCTTGCACT25340.22804155143848864No Hit
GTGTGTGTGTGTGTGACAGGGTGAGGCTGGAGGCTCCTCTGACTGTTCTT24830.2234519227394504No Hit
GTATACACATATTGTGTAATGTGTATGTGTGTTATGTTTCTGTCTGGAGG23970.21571254885479765No Hit
GGGCAGTGTGTGCGTGTGTGGAAGATGGAACGGGATGGCAAACAGTGACA23670.2130127672671281No Hit
CTCTTGTGGTGTTTATGCAAAAGCCGGTCACGTGAAGTCTGCAAATGTAC21870.19681407774111073No Hit
GGTTGGCAGGGTGAGGTTGGCAGGGTGAGGCTGGCAGGGTGAGGTTGGCA21490.1933943543967293No Hit
CATTTAAGGAGCGCAGAGCTGAGAGTCAGCCGGGCGTCTGATTGGTGGCG21470.19321436895755134No Hit
AAAGCAGGGTGAGGTTGGCAGGGTGAGGTTGGCAGGGTGAGGCTGGCAGG20740.1866449004275554No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT19750.17773562118824587No Hit
CATATAATGCATACATATGCATTGTAATCATTTTGTGTTAAAAAAAAGAC19410.17467586872222038No Hit
CATAAAACAGCGTGCCGGGTCCCAAACAGACGGAACATTCATTTAGGTTT19260.1733259779283856No Hit
ATATAGAACAGCAAACGCAATTACCCCATCAACAAGTTAAGCTGTTGAAA18710.1683763783509914No Hit
ATTATGCACCGTTGAAAAAAGGCCCAGGTTTTGCCCAGCTGCCTCACTGC18530.16675650939838965No Hit
GCAGGGCAGTGGGCAGTGTGTGCGTGTGTGGAAGATGGAACGGGATGGCA18410.16567659676332186No Hit
GGGCAGTGGGCAGTGTGTGCGTGTGTGGAAGATGGAACGGGATGGCAAAC18310.164776669567432No Hit
GGGTGAGGCTGGCAGGGTGAGGTTGGCAGGGTGAGGCTGGCAGGGTGAGG18210.16387674237154215No Hit
GATTTATATTTTATTGATCATTTCACCTGTGAATAAATTATGCACCGTTG17970.1617169171014065No Hit
AGGCTGGAGTGTGTGTGTGTGTGTGACAGGGTGAGGCTGGAGGCTCCTCT17680.15910712823332593No Hit
GGGTGAGGTTGGCAGGGTGAGGCTGGCAGGGTGAGGTTGGCAGGGTGAGG17580.15820720103743607No Hit
GCATAATGCACCCCTGCAGAAGCCCCCTGCATTTGCGTAAGCAGCCACAT16480.1483080018826477No Hit
GTGTGTGACAGGGTGAGGCTGGAGGCTCCTCTGACTGTTCTTTACAATGA16460.1481280164434697No Hit
GGGTGTACAGTCAGGAGGAACAATGCCATCTGCGTGGGGGCCATTCACCG16420.14776804556511378No Hit
GTGTGTGTGTGTGTGCCTGTGTGTGTCAAGGGAAGAGGGGGGAAGGGTGT15640.14074861343717293No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACA15400.1385887881670373No Hit
GTCCAGGAGGTCTGTGCTCCAGGAGGTCTGTGCTCCATCTTCACGGAGGA15100.13588900657936773No Hit
ATGCATATGTATGCATTATATGTGTGCTTTTGGGGTGTAGATGTGAGGAC14860.1337291813092321No Hit
ATACAGGGAAGAGGATCCTGTCAACCTACGAAGATCACTCCCTCCCTCTC14530.13075942156279557No Hit
GTTTTAACAACGCTCACAACACAGGGAGATCAGATCAGATCAGAACGTGC14420.12976950164731674No Hit
GTTCTTTACAATGAGAGGGAGGGAGTGATCTTCGTAGGTTGACAGGATCC14360.1292295453297828No Hit
GTATGTATGTGTGTACCTGAGCTTGCTCTTCAAGGTGTTCACTTCGCGGT14240.128149632694715No Hit
GTGGGCAGTGTGTGCGTGTGTGGAAGATGGAACGGGATGGCAAACAGTGA14200.12778966181635906No Hit
GTGTGTGTGTGTGTGTGCCTGTGTGTGTCAAGGGAAGAGGGGGGAAGGGT14040.12634977830293528No Hit
GTGTGTGTGTGTGTGTGACAGGGTGAGGCTGGAGGCTCCTCTGACTGTTC13710.12338001855649879No Hit
GGGTGAGGTTGGCAGGGTGAGGCTGGCAGGGTGAGGCTGGAGTGTGTGTG13680.12311004039773184No Hit
CTTCAGAGTCACCTCGGTTTGGGATTACAAATCTTTGACCAAGGACCACA13510.12158016416471908No Hit
GCATATGTATGCATTATATGTGTGCTTTTGGGGTGTAGATGTGAGGACAT13070.11762048450280373No Hit
GTGTGTGTGTGTGACAGGGTGAGGCTGGAGGCTCCTCTGACTGTTCTTTA13040.11735050634403676No Hit
CCAAATCAACTGTCCGAGCTGCCACCATGTCAGCCGATCACAATACAGGG12930.11636058642855794No Hit
TATATACAGTATGTTACCAAGACACAGCAAAAAGATAACTATGCTACACG12920.11627059370896894No Hit
CTGTAGGCAAGCATAAGTGACTTGAACTTAATGCGAGCAGCTACCGGCAG12890.11600061555020198No Hit
TCACAATACAGGGAAGAGGATCCTGTCAACCTACGAAGATCACTCCCTCC12730.11456073203677823No Hit
GGTTGGCAGGGTGAGGCTGGCAGGGTGAGGTTGGCAGGGTGAGGCTGGCA12700.11429075387801126No Hit
CCTCTGACTGTTCTTTACAATGAGAGGGAGGGAGTGATCTTCGTAGGTTG12610.1134808194017104No Hit
CTCTTGTGGTGTTTAGGCAAAAGCCGGTCACGTGAAGTCTGCAAATGTAC12420.11177095772951968No Hit
CAATACAGGGAAGAGGATCCTGTCAACCTACGAAGATCACTCCCTCCCTC12410.11168096500993069No Hit
GAGTTATATCATAGTCTTCATGAACATACCCGAAGCTCCTTTGTTATTAT12410.11168096500993069No Hit
GAGGTATACTTTGTAGTAAAACAAAGTAATCTGTGTGTGTGTGTGTGTGT12110.10898118342226114No Hit
GTGTGTGTGTGTGCCTGTGTGTGTCAAGGGAAGAGGGGGGAAGGGTGTTA11880.10691135087171448No Hit
GGATGGCAAACAGTGACATCACCAAATCAACTGTCCGAGCTGCCACCATG11780.10601142367582463No Hit
ATCGAAACCTGTGTCCCCGCGGGCATTGTAGCCCGTAAATGGGGGGCTTA11750.10574144551705768No Hit
GGGCTAATCCTGTGCATGCAGCACGACACGAAGAACACCGACAAACTGGA11740.1056514527974687No Hit
AAGCAGGGCAGCCTTGCGGAGACAGGTGGTGACACTCGAGGTCCACTCTG11730.1055614600778797No Hit
GTCCAATATCAGGCTGTATACTGTTATTGTATTTGCTACAGTTATATGCC11680.10511149647993477No Hit
GTATACTGTTATTGTATTTGCTACAGTTATATGCCATTATGAGCTTGTCG11660.10493151104075679No Hit
GGGTGAGGCTGGAGGCTCCTCTGACTGTTCTTTACAATGAGAGGGAGGGA11620.10457154016240086No Hit
AGCGAAAGCAGGGCAGCCTTGCGGAGACAGGTGGTGACACTCGAGGTCCA11570.10412157656445593No Hit
CATTATAAGAGGTATACTTTGTAGTAAAACAAAGTAATCTGTGTGTGTGT11390.1025017076118542No Hit
GTGGAAGATGGAACGGGATGGCAAACAGTGACATCACCAAATCAACTGTC11350.10214173673349827No Hit
GTCTGTGATAATACATCACAGGCGGCACATTGTATATCAGAAATCAATGA11340.10205174401390928No Hit
GGGTGAGGCTGGCAGGGTGAGGCTGGAGTGTGTGTGTGTGTGTGACAGGG11290.10160178041596435No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA19750.0120.639971
GATCACC2900.0105.0884145
GCGAAAG25450.093.620412
CGAAAGC28500.083.60893
GGGATAC451.2410328E-680.4673841
TACTCCC1650.079.1575145
GAGCAAA3200.076.946941
TACTGGG1700.076.701397
TGGTTCC502.2949025E-672.56104145
GCTCGGG300.001929346172.56104145
GACATAA1850.070.463331
ACGCTCC451.1889536E-464.4987145
CCCGGCC451.1889536E-464.4987145
ACGTGCC808.034476E-963.490913145
GATACAA606.772205E-660.467533145
CGCGTCC606.772205E-660.467533145
CGACGAA2800.057.012253145
GATAATT1051.1204975E-955.177642
AGGCCGT1450.054.9546366
TAGAAAC1750.053.7981952
Produced by FastQC (version 0.11.5)