FastQCFastQC Report
Tue 2 Jul 2019
HWC5JBCX2_l02_n02_SM2_9608_P2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l02_n02_SM2_9608_P2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences253849
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTAATTCTGTGGTAATTTCATGGGCAACATAATCCAATTGTGACCGTT10660.41993468558079805No Hit
GGTTGAGGGCGACATACCCGCAGAATGAAAAACAAGGAGGGAGTGAGTCA10330.4069348313367396No Hit
GTGTATATGAGGGAGGTGGACTTTGTTTCTCCCTCTCTTCTCTGGAAACA9420.3710867484213056No Hit
GCATTAATAATAATAATCATAAATTCAGCCACAGCCTTTGAACGTGTGTG9160.3608444390168959No Hit
TTGCTGTACTGCTCCCCCTGATTAAAAACCAACTTCTGCCTTCGCTGGAA8110.31948126642216435No Hit
GTAAATAACCAGGCCCACCAATTTGCCTTTCTCTCGCCTCCAAATGATGA7880.3104207619490327No Hit
CTATAGAAGTGTGTGTTGTTGTTGGTGTCTGGGACACAAATATTCGTCAC7610.29978451756753033No Hit
AGCAGGGGCTGTGACAAAATCACACACGCCAGTTGGCAACCTGCAAGAAA6950.2737848090794134No Hit
GGATAGCATTAATAATAATAATCATAAATTCAGCCACAGCCTTTGAACGT6490.25566380013315004No Hit
CTTCTATAGCGTTTAAGCGTTTGGACGTAGAACATTTGGAAAACGGTACA6180.2434518158432769No Hit
CTATTTAAGTTATTTAATTTCTCCTGGAGAGGGAGGAGTGACTGGGAACT6010.2367549212327013No Hit
TTTCATTCCTGTGCAGCCTTTTCCTGTTTAGTGCTCACATAGTTTGCGAT5960.2347852463472379No Hit
AAGCAGGAGAGAGCGCGAGAGAGCAGAGAGGAGAGAGATTGGTCTTGTTG5910.23281557146177453No Hit
GTATATGAGGGAGGTGGACTTTGTTTCTCCCTCTCTTCTCTGGAAACATC5900.23242163648468186No Hit
GGAATGAAAAGCCTCCATCTCGCTTCTGTCCTTTAACAAATGAGCATAAT5610.2209975221489941No Hit
AGCAGGTTGAGGGCGACATACCCGCAGAATGAAAAACAAGGAGGGAGTGA5590.22020965219480873No Hit
CATATACACACCTCCCCCTTCTTCTTTCACTTAGTTCACACTCCAGATAG5580.21981571721771606No Hit
GAATATTACAGGTGGTCCTATCTGCCTAAATTTTCTGCTCAAAAAACAGA5420.21351275758423313No Hit
CTGTTATACTCAGGATGTGAAAATGAAGGAACTAAAGTTGGGGTGGGGGG5410.21311882260714046No Hit
ATCTAATGCTGTTATACTCAGGATGTGAAAATGAAGGAACTAAAGTTGGG5380.21193701767586245No Hit
GTTATACTCAGGATGTGAAAATGAAGGAACTAAAGTTGGGGTGGGGGGAA5240.20642192799656486No Hit
TATCTGGAGTGTGAACTAAGTGAAAGAAGAAGGGGGAGGTGTGTATATGA5160.20327044817982343No Hit
ACTTATTCCTGGTCCTATCTGGAGTGTGAACTAAGTGAAAGAAGAAGGGG5140.2024825782256381No Hit
AAAGCAGGTTGAGGGCGACATACCCGCAGAATGAAAAACAAGGAGGGAGT5080.200118968363082No Hit
AGCGAAAGCAGGTTGAGGGCGACATACCCGCAGAATGAAAAACAAGGAGG5020.1977553585005259No Hit
ATTTAAGTCTGGACTAAGGTTGAACTTTAACAGAAATGGCAGTCAGGCCA5010.19736142352343322No Hit
CTTCCAGGCTGGGAGAACGTGAACACAAAAAAACCGCCATCGCTGAGCGG4900.19302813877541372No Hit
GTGTGTTCTCCGTCTGAGCTCCATGAGTCAGCTGGGAGGGGAGAGGCCGA4850.19105846388995032No Hit
GTTGTATAGAATGGAGGTGTCTTCCTACCTGGAGAGAGACTTTCCTAATC4810.18948272398157961No Hit
GTGTGAGGATTACAAAAACAAAATAAAAAGGGTTGCTTCATCATTTGGAG4740.18672517914193082No Hit
TATTACAGGTGGTCCTATCTGCCTAAATTTTCTGCTCAAAAAACAGAATC4660.18357369932518938No Hit
GTTTTAAATTGAGGTAAAAATCGCAAACTATGTGAGCACTAAACAGGAAA4640.18278582937100402No Hit
CTATACAACCTCCCAGCACTGTTCTCCCTCACTGCCTTATCCAAAGGAGC4630.18239189439391135No Hit
GACATACACAGGTATTTCTGCCAAGGGATCAAATCCTTGCGGGACCCACC4550.1792404145771699No Hit
TCTCTGCATTGTGTGTTCTCCGTCTGAGCTCCATGAGTCAGCTGGGAGGG4550.1792404145771699No Hit
CACATGCACCTGCCTTTGTTTCTGACTGTCGGCGATTCTGACAAGGGCAT4540.1788464796000772No Hit
CATATAGCTTGAGCTGTTTTCACCATGGCCCCGTGCTTGCTAATGTCATG4490.1768768047146138No Hit
GTGAATAGCAGTCCTCTGAGGCACACAGTGTGTTTCTAAGTCTTGTCCAC4490.1768768047146138No Hit
CTGTTAGAACGTATGAGCTGTGAATAGCAGTCCTCTGAGGCACACAGTGT4460.17569499978333578No Hit
CCATAATTGGACGAGGCTGCAGCTGCTTCTCTCTGCATTGTGTGTTCTCC4450.17530106480624308No Hit
CTATTAATATTACACGAGTACTTTAAAGGGAGAATGGAGGACAAATGGAG4440.1749071298291504No Hit
GTCTTACATATGGTGTTTGCTCCTCTGGGTGTTTTTAACTACTTTTTTCT4410.17372532489787235No Hit
TACATATGGTGTTTGCTCCTCTGGGTGTTTTTAACTACTTTTTTCTAACT4330.1705738450811309No Hit
CCATTTATGCTGATGTGATTTCACCGCCTGTAAGGTCATGTAGTAGGTGC4310.16978597512694554No Hit
GGCAATGGCGTGTGTGTGCTTGGGGACCTTGTGTGTTTCTTTGGTGTTGT4300.16939204014985287No Hit
CTTCAGAGTCACCTCGGTTTGGGATTACAAATCTTTGACCAAGGACCACA4300.16939204014985287No Hit
GTATGAGTCTGTCTTTTTAGAAACTGTTTCTTCTAGTGAAATTATGATAT4280.1686041701956675No Hit
GTATAGAATGGAGGTGTCTTCCTACCTGGAGAGAGACTTTCCTAATCCGG4130.1626951455392773No Hit
GGTTATTTACAGTGACTTTTGCAGACTTTTGCTAAATGTGGAAGATTATG4100.16151334060799924No Hit
CCTTTGGCTTGGTAATTTAGCACCATAATTGGACGAGGCTGCAGCTGCTT4070.1603315356767212No Hit
GTATAACAGCATTAGATATAGAAAAACAGTCTTAGAAATAACTCCTATGA3840.15127103120358953No Hit
ATCCAAAGGAGCAGGGGCTGCTTTACTCGTGTCCCCGGCACAGTTAATTG3800.14969529129521883No Hit
CTTAAATAGTGTTTCATTGAAATGGATCTCATTCGAAATCATATCCTAAG3770.14851348636394077No Hit
GCAGGAGAGAGCGCGAGAGAGCAGAGAGGAGAGAGATTGGTCTTGTTGCT3760.14811955138684807No Hit
ATCCAATTGTGACCGTTAAAAAAACCACCACACACAAACTAGCACACGTA3760.14811955138684807No Hit
CTTGGGGACCTTGTGTGTTTCTTTGGTGTTGTGAGAGAAATGGGAGTCGG3760.14811955138684807No Hit
CTATGTGAGCACTAAACAGGAAAAGGCTGCACAGGAATGAAAAGCCTCCA3750.1477256164097554No Hit
ACATAATCCAATTGTGACCGTTAAAAAAACCACCACACACAAACTAGCAC3740.14733168143266273No Hit
TCCTCACACAAAGCACCCAAGGCGACCAAGTCCTCCGGATTAGGAAAGTC3720.14654381147847736No Hit
GTGTGTGTGTGTGTCTCACACTCTCTGTCTCTCTCTGTCTTCCTTTGAGC3710.1461498765013847No Hit
CTGCTGGACTCCTTGATTTTTTTTTAATCATTGTTTGATTTTGAGCAGTA3700.145755941524292No Hit
TAATTGGACGAGGCTGCAGCTGCTTCTCTCTGCATTGTGTGTTCTCCGTC3700.145755941524292No Hit
ATGAAAAACAAGGAGGGAGTGAGTCACCTAAAACGGCTGGAGGTCGGGGG3670.14457413659301396No Hit
ATATTACACGAGTACTTTAAAGGGAGAATGGAGGACAAATGGAGCGTGAA3640.14339233166173593No Hit
CCCCTACTATTGTAAATGACGTTCATTCAGTGGAGAATTACTAGATGTAA3630.14299839668464323No Hit
GCCTTTGAACGTGTGTGTGTGTGTGTGTGTGCGTACGTGTGCTAGTTTGT3630.14299839668464323No Hit
CTTTAAAGGGAGAATGGAGGACAAATGGAGCGTGAAGCAGCAGCGAGCGC3550.1398469168679018No Hit
GTATTTGCTGTACTGCTCCCCCTGATTAAAAACCAACTTCTGCCTTCGCT3520.13866511193662373No Hit
CCTAAAACGGCTGGAGGTCGGGGGTCAATGTAATTCTGTGGTAATTTCAT3510.13827117695953106No Hit
GGCTGGGAGAACGTGAACACAAAAAAACCGCCATCGCTGAGCGGAAAAGA3510.13827117695953106No Hit
GAATGGAGGTGTCTTCCTACCTGGAGAGAGACTTTCCTAATCCGGAGGAC3480.13708937202825303No Hit
CTCCTGGAGAGGGAGGAGTGACTGGGAACTCAGACCAGGTGGATTCCTTG3430.1351196971427896No Hit
CGCCAGTTGGCAACCTGCAAGAAACAGCAAGGATAGCATTAATAATAATA3400.13393789221151156No Hit
GTGTGTTTCTAAGTCTTGTCCACACTAACCTGGGGAAATTTGAAAAAACA3390.1335439572344189No Hit
ACCTAAAACGGCTGGAGGTCGGGGGTCAATGTAATTCTGTGGTAATTTCA3380.13315002225732622No Hit
ATTTAAAACATACGCCATCAATCTTCAAATACACTAAACTTATCCTTTCC3360.13236215230314083No Hit
GTACCTGAGTGGTTTCTTCTCAGTCTTCACGCTCTCAACTGTGTCAAGCT3330.1311803473718628No Hit
ATATGAGGGAGGTGGACTTTGTTTCTCCCTCTCTTCTCTGGAAACATCAT3310.13039247741767745No Hit
GAGTTATATCATAGTCTTCATGAACATACCCGAAGCTCCTTTGTTATTAT3290.1296046074634921No Hit
GTCCAATATCAGGCTGTATACTGTTATTGTATTTGCTACAGTTATATGCC3260.12842280253221403No Hit
GTCATGTAGTAGGTGCTCATTTGTTAACTGACACAACTGCTAATTCTCAA3250.12802886755512136No Hit
CTATTGTTTTTGTACCGTTTTCCAAATGTTCTACGTCCAAACGCTTAAAC3240.12763493257802866No Hit
GGATATGATTTCGAATGAGATCCATTTCAATGAAACACTATTTAAGTTAT3240.12763493257802866No Hit
CAGCAAGGATAGCATTAATAATAATAATCATAAATTCAGCCACAGCCTTT3180.1252713227154726No Hit
CCTTTGGATAAGGCAGTGAGGGAGAACAGTGCTGGGAGGTTGTATAGAAT3100.12211984289873114No Hit
GCATTAGATATAGAAAAACAGTCTTAGAAATAACTCCTATGAACCACAAC3080.12133197294454576No Hit
GTGTGTATGGAGGGAGATTCTAAATGCCTTAATTAAAATTGTAGGAGTCT3080.12133197294454576No Hit
GTTAGAACGTATGAGCTGTGAATAGCAGTCCTCTGAGGCACACAGTGTGT3050.12015016801326774No Hit
GTGTGTGCTTGGGGACCTTGTGTGTTTCTTTGGTGTTGTGAGAGAAATGG3040.11975623303617504No Hit
TCAATTAACTGTGCCGGGGACACGAGTAAAGCAGCCCCTGCTCCTTTGGA3020.1189683630819897No Hit
GTCCTGGAAACACATCAAGCTCAGCTCCTGTGTCCAGCTCGCTTCTCTGC3020.1189683630819897No Hit
GTGCTGGGAGGTTGTATAGAATGGAGGTGTCTTCCTACCTGGAGAGAGAC3020.1189683630819897No Hit
CTCTGTCTCTCTCTGTCTTCCTTTGAGCGCCTTTGGCTTGGTAATTTAGC3010.118574428104897No Hit
GTTTAGTGTATTTGAAGATTGATGGCGTATGTTTTAAATTGAGGTAAAAA3010.118574428104897No Hit
GTGAAACACTGTTAGAACGTATGAGCTGTGAATAGCAGTCCTCTGAGGCA3010.118574428104897No Hit
TGTAGTAGGTGCTCATTTGTTAACTGACACAACTGCTAATTCTCAAAGGG3000.11818049312780432No Hit
GTATACTGTTATTGTATTTGCTACAGTTATATGCCATTATGAGCTTGTCG2980.11739262317361895No Hit
GAGTATAACAGCATTAGATATAGAAAAACAGTCTTAGAAATAACTCCTAT2980.11739262317361895No Hit
CTCCAGATAGGACCAGGAATAAGTTGTCTGGGCTTTTATTTCCCCCCACC2970.11699868819652628No Hit
GGCTGTGACAAAATCACACACGCCAGTTGGCAACCTGCAAGAAACAGCAA2960.1166047532194336No Hit
TTCTTAAACTCTCTAATTTTCCCTGTCCACATTACAACGCAAACCCGGCG2950.11621081824234093No Hit
GTCCTATCTGGAGTGTGAACTAAGTGAAAGAAGAAGGGGGAGGTGTGTAT2910.11463507833397019No Hit
AGCGAAAGCAGGGGCTGTGACAAAATCACACACGCCAGTTGGCAACCTGC2880.11345327340269215No Hit
CCTTTGAAAAACGCCGGGTTTGCGTTGTAATGTGGACAGGGAAAATTAGA2880.11345327340269215No Hit
TGCCTACACCGCCTCATAATAATTGATATTCATGACTATTAATATTACAC2860.1126654034485068No Hit
ATTCTATACAACCTCCCAGCACTGTTCTCCCTCACTGCCTTATCCAAAGG2860.1126654034485068No Hit
CATCAGAGTTATATCATAGTCTTCATGAACATACCCGAAGCTCCTTTGTT2820.11108966354013608No Hit
TTCCTAATCCGGAGGACTTGGTCGCCTTGGGTGCTTTGTGTGAGGATTAC2820.11108966354013608No Hit
GCTCTCTATGGGCTGACATTTTACCTCATTATCTGAAATTGGATACTGGA2820.11108966354013608No Hit
ATGCAGAGAGAAGCAGCTGCAGCCTCGTCCAATTATGGTGCTAAATTACC2810.11069572856304338No Hit
CATCATGGTAAACTCCCCATGGTTCTTTTTGCAGAAATAGTCACCTAAAT2770.10911998865467266No Hit
CCTATTCATCCATGGTGAGTTTGGATTCACGTGTTACCAGAATTGCATGC2760.10872605367757998No Hit
GTATAGCGAGTGGCGACAAGCTCATAATGGCATATAACTGTAGCAAATAC2730.10754424874630195No Hit
GGCTTAGGATATGATTTCGAATGAGATCCATTTCAATGAAACACTATTTA2720.10715031376920925No Hit
CCCCATGGTTCTTTTTGCAGAAATAGTCACCTAAATTTCCAAACAGCAAT2690.10596850883793123No Hit
CTTTAACACAGCTCTCTATGGGCTGACATTTTACCTCATTATCTGAAATT2690.10596850883793123No Hit
CCTCTGAGGCACACAGTGTGTTTCTAAGTCTTGTCCACACTAACCTGGGG2680.10557457386083853No Hit
GTTATATGCCATTATGAGCTTGTCGCCACTCGCTATACAGCCCAACAAAC2660.10478670390665318No Hit
CCTATGAACCACAACTCAACTTAATTCATCATTGGCTTAGGATATGATTT2650.10439276892956048No Hit
TCCCAGCACTGTTCTCCCTCACTGCCTTATCCAAAGGAGCAGGGGCTGCT2650.10439276892956048No Hit
CAAGGAGGGAGTGAGTCACCTAAAACGGCTGGAGGTCGGGGGTCAATGTA2650.10439276892956048No Hit
ATATTACAGGTGGTCCTATCTGCCTAAATTTTCTGCTCAAAAAACAGAAT2650.10439276892956048No Hit
GTTGAGGGCGACATACCCGCAGAATGAAAAACAAGGAGGGAGTGAGTCAC2650.10439276892956048No Hit
CTCTCTGTCTCTCTCTGTCTTCCTTTGAGCGCCTTTGGCTTGGTAATTTA2650.10439276892956048No Hit
ACTATAGCCTCTCCAGCAGAGCTTTCTATGGGCTGAGAGCCACTGAATGC2650.10439276892956048No Hit
GCTTTAACACAGCTCTCTATGGGCTGACATTTTACCTCATTATCTGAAAT2640.10399883395246781No Hit
ATTGTAAATGACGTTCATTCAGTGGAGAATTACTAGATGTAATCAGACGA2620.10321096399828243No Hit
ATCATATCCTAAGCCAATGATGAATTAAGTTGAGTTGTGGTTCATAGGAG2610.10281702902118976No Hit
GGATAAGTTTAGTGTATTTGAAGATTGATGGCGTATGTTTTAAATTGAGG2600.10242309404409708No Hit
GTCCTATCTGCCTAAATTTTCTGCTCAAAAAACAGAATCCGCAGCTCGGT2590.1020291590670044No Hit
GCATTGTGTGTTCTCCGTCTGAGCTCCATGAGTCAGCTGGGAGGGGAGAG2550.10045341915863368No Hit
GTCTTAGAAATAACTCCTATGAACCACAACTCAACTTAATTCATCATTGG2550.10045341915863368No Hit
GTCTCACACTCTCTGTCTCTCTCTGTCTTCCTTTGAGCGCCTTTGGCTTG2550.10045341915863368No Hit
ACCATGGACAATAATGAGACTATTTCTTTTCTTTTCTATTCTTTTCTTGT2540.100059484181541No Hit
AAATCACACACGCCAGTTGGCAACCTGCAAGAAACAGCAAGGATAGCATT2540.100059484181541No Hit
ATCAAAGACAACTTCTTCTCAATATTATTTTTTTGAGCCCACATGCACCT2540.100059484181541No Hit
TACCCGCAGAATGAAAAACAAGGAGGGAGTGAGTCACCTAAAACGGCTGG2540.100059484181541No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCGGG100.00706908145.12805145
ACATCTT100.00706908145.12805145
GATCTCC100.00706908145.12805145
ACTGGTC100.0070941355144.956448
GACACCC800.0126.98705145
TGACGCC256.427221E-6116.10245145
GGGTTGA458.54925E-11112.721686
AGCGAAA2400.0108.674491
GGGGTTG501.9463187E-10101.449525
GAGCGAA554.1654857E-1092.208671
GCGAAAG3050.085.5143662
TATACGA353.4408054E-582.815935
CGAAAGC3200.081.5058753
GTCCTGG451.2353194E-680.4996261
TTGAGTG651.5625119E-978.0534749
CCATGCC300.001936378472.478228
ATACGAA406.670036E-572.463946
TTATACG406.6765526E-572.449664
GAAAGCA3600.072.449664
GCAGGAG1250.069.551683
Produced by FastQC (version 0.11.5)