FastQCFastQC Report
Thu 11 Jul 2019
HWC5JBCX2_l02_n02_SM2_9608_P2_DM2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l02_n02_SM2_9608_P2_DM2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences255531
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTAATTCTGTGGTAATTTCATGGGCAACATAATCCAATTGTGACCGTT10760.421083938935002No Hit
GGTTGAGGGCGACATACCCGCAGAATGAAAAACAAGGAGGGAGTGAGTCA10340.4046475770063123No Hit
GTGTATATGAGGGAGGTGGACTTTGTTTCTCCCTCTCTTCTCTGGAAACA9460.3702094853462007No Hit
GCATTAATAATAATAATCATAAATTCAGCCACAGCCTTTGAACGTGTGTG9230.3612086204804897No Hit
TTGCTGTACTGCTCCCCCTGATTAAAAACCAACTTCTGCCTTCGCTGGAA8140.3185523478560331No Hit
GTAAATAACCAGGCCCACCAATTTGCCTTTCTCTCGCCTCCAAATGATGA7930.31033416689168825No Hit
CTATAGAAGTGTGTGTTGTTGTTGGTGTCTGGGACACAAATATTCGTCAC7620.29820256642051257No Hit
AGCAGGGGCTGTGACAAAATCACACACGCCAGTTGGCAACCTGCAAGAAA6980.27315668157679496No Hit
GGATAGCATTAATAATAATAATCATAAATTCAGCCACAGCCTTTGAACGT6550.25632897769742224No Hit
CTTCTATAGCGTTTAAGCGTTTGGACGTAGAACATTTGGAAAACGGTACA6220.24341469332488036No Hit
CTATTTAAGTTATTTAATTTCTCCTGGAGAGGGAGGAGTGACTGGGAACT6040.23637053821258477No Hit
TTTCATTCCTGTGCAGCCTTTTCCTGTTTAGTGCTCACATAGTTTGCGAT5980.23402248650848623No Hit
GTATATGAGGGAGGTGGACTTTGTTTCTCCCTCTCTTCTCTGGAAACATC5940.2324571187057539No Hit
AAGCAGGAGAGAGCGCGAGAGAGCAGAGAGGAGAGAGATTGGTCTTGTTG5920.2316744348043877No Hit
GGAATGAAAAGCCTCCATCTCGCTTCTGTCCTTTAACAAATGAGCATAAT5620.21993417628389508No Hit
AGCAGGTTGAGGGCGACATACCCGCAGAATGAAAAACAAGGAGGGAGTGA5610.21954283433321203No Hit
CATATACACACCTCCCCCTTCTTCTTTCACTTAGTTCACACTCCAGATAG5610.21954283433321203No Hit
GAATATTACAGGTGGTCCTATCTGCCTAAATTTTCTGCTCAAAAAACAGA5430.21249867922091642No Hit
CTGTTATACTCAGGATGTGAAAATGAAGGAACTAAAGTTGGGGTGGGGGG5430.21249867922091642No Hit
ATCTAATGCTGTTATACTCAGGATGTGAAAATGAAGGAACTAAAGTTGGG5410.21171599531955027No Hit
GTTATACTCAGGATGTGAAAATGAAGGAACTAAAGTTGGGGTGGGGGGAA5240.20506318215793776No Hit
TATCTGGAGTGTGAACTAAGTGAAAGAAGAAGGGGGAGGTGTGTATATGA5200.20349781435520542No Hit
ACTTATTCCTGGTCCTATCTGGAGTGTGAACTAAGTGAAAGAAGAAGGGG5160.2019324465524731No Hit
AAAGCAGGTTGAGGGCGACATACCCGCAGAATGAAAAACAAGGAGGGAGT5090.19919305289769146No Hit
ATTTAAGTCTGGACTAAGGTTGAACTTTAACAGAAATGGCAGTCAGGCCA5070.19841036899632528No Hit
AGCGAAAGCAGGTTGAGGGCGACATACCCGCAGAATGAAAAACAAGGAGG5020.19645365924290986No Hit
CTTCCAGGCTGGGAGAACGTGAACACAAAAAAACCGCCATCGCTGAGCGG4930.19293158168676205No Hit
GTGTGTTCTCCGTCTGAGCTCCATGAGTCAGCTGGGAGGGGAGAGGCCGA4850.18980084608129738No Hit
GTTGTATAGAATGGAGGTGTCTTCCTACCTGGAGAGAGACTTTCCTAATC4830.1890181621799312No Hit
GTGTGAGGATTACAAAAACAAAATAAAAAGGGTTGCTTCATCATTTGGAG4780.18706145242651576No Hit
TATTACAGGTGGTCCTATCTGCCTAAATTTTCTGCTCAAAAAACAGAATC4670.1827566909690018No Hit
CTATACAACCTCCCAGCACTGTTCTCCCTCACTGCCTTATCCAAAGGAGC4650.18197400706763564No Hit
GTTTTAAATTGAGGTAAAAATCGCAAACTATGTGAGCACTAAACAGGAAA4640.18158266511695254No Hit
GACATACACAGGTATTTCTGCCAAGGGATCAAATCCTTGCGGGACCCACC4570.1788432714621709No Hit
TCTCTGCATTGTGTGTTCTCCGTCTGAGCTCCATGAGTCAGCTGGGAGGG4560.17845192951148786No Hit
CACATGCACCTGCCTTTGTTTCTGACTGTCGGCGATTCTGACAAGGGCAT4550.17806058756080476No Hit
CATATAGCTTGAGCTGTTTTCACCATGGCCCCGTGCTTGCTAATGTCATG4520.1768865617087555No Hit
GTGAATAGCAGTCCTCTGAGGCACACAGTGTGTTTCTAAGTCTTGTCCAC4510.17649521975807242No Hit
CTGTTAGAACGTATGAGCTGTGAATAGCAGTCCTCTGAGGCACACAGTGT4500.17610387780738931No Hit
CCATAATTGGACGAGGCTGCAGCTGCTTCTCTCTGCATTGTGTGTTCTCC4480.17532119390602313No Hit
CTATTAATATTACACGAGTACTTTAAAGGGAGAATGGAGGACAAATGGAG4460.17453851000465698No Hit
GTCTTACATATGGTGTTTGCTCCTCTGGGTGTTTTTAACTACTTTTTTCT4450.17414716805397387No Hit
TACATATGGTGTTTGCTCCTCTGGGTGTTTTTAACTACTTTTTTCTAACT4340.16984240659645994No Hit
CCATTTATGCTGATGTGATTTCACCGCCTGTAAGGTCATGTAGTAGGTGC4330.16945106464577683No Hit
GGCAATGGCGTGTGTGTGCTTGGGGACCTTGTGTGTTTCTTTGGTGTTGT4320.16905972269509376No Hit
CTTCAGAGTCACCTCGGTTTGGGATTACAAATCTTTGACCAAGGACCACA4320.16905972269509376No Hit
GTATGAGTCTGTCTTTTTAGAAACTGTTTCTTCTAGTGAAATTATGATAT4310.16866838074441065No Hit
GTATAGAATGGAGGTGTCTTCCTACCTGGAGAGAGACTTTCCTAATCCGG4140.16201556758279817No Hit
GGTTATTTACAGTGACTTTTGCAGACTTTTGCTAAATGTGGAAGATTATG4120.161232883681432No Hit
CCTTTGGCTTGGTAATTTAGCACCATAATTGGACGAGGCTGCAGCTGCTT4070.15927617392801655No Hit
GTATAACAGCATTAGATATAGAAAAACAGTCTTAGAAATAACTCCTATGA3840.15027530906230557No Hit
ATCCAAAGGAGCAGGGGCTGCTTTACTCGTGTCCCCGGCACAGTTAATTG3820.1494926251609394No Hit
GCAGGAGAGAGCGCGAGAGAGCAGAGAGGAGAGAGATTGGTCTTGTTGCT3780.14792725735820703No Hit
ATCCAATTGTGACCGTTAAAAAAACCACCACACACAAACTAGCACACGTA3780.14792725735820703No Hit
CTTGGGGACCTTGTGTGTTTCTTTGGTGTTGTGAGAGAAATGGGAGTCGG3780.14792725735820703No Hit
CTTAAATAGTGTTTCATTGAAATGGATCTCATTCGAAATCATATCCTAAG3770.14753591540752395No Hit
ACATAATCCAATTGTGACCGTTAAAAAAACCACCACACACAAACTAGCAC3760.14714457345684084No Hit
CTATGTGAGCACTAAACAGGAAAAGGCTGCACAGGAATGAAAAGCCTCCA3760.14714457345684084No Hit
TCCTCACACAAAGCACCCAAGGCGACCAAGTCCTCCGGATTAGGAAAGTC3730.14597054760479158No Hit
GTGTGTGTGTGTGTCTCACACTCTCTGTCTCTCTCTGTCTTCCTTTGAGC3720.1455792056541085No Hit
TAATTGGACGAGGCTGCAGCTGCTTCTCTCTGCATTGTGTGTTCTCCGTC3710.1451878637034254No Hit
CTGCTGGACTCCTTGATTTTTTTTTAATCATTGTTTGATTTTGAGCAGTA3700.14479652175274232No Hit
ATGAAAAACAAGGAGGGAGTGAGTCACCTAAAACGGCTGGAGGTCGGGGG3680.14401383785137614No Hit
CCCCTACTATTGTAAATGACGTTCATTCAGTGGAGAATTACTAGATGTAA3650.1428398119993269No Hit
ATATTACACGAGTACTTTAAAGGGAGAATGGAGGACAAATGGAGCGTGAA3640.1424484700486438No Hit
GCCTTTGAACGTGTGTGTGTGTGTGTGTGTGCGTACGTGTGCTAGTTTGT3630.14205712809796073No Hit
CTTTAAAGGGAGAATGGAGGACAAATGGAGCGTGAAGCAGCAGCGAGCGC3580.14010041834454529No Hit
CCTAAAACGGCTGGAGGTCGGGGGTCAATGTAATTCTGTGGTAATTTCAT3520.13775236664044674No Hit
GGCTGGGAGAACGTGAACACAAAAAAACCGCCATCGCTGAGCGGAAAAGA3520.13775236664044674No Hit
GTATTTGCTGTACTGCTCCCCCTGATTAAAAACCAACTTCTGCCTTCGCT3520.13775236664044674No Hit
GAATGGAGGTGTCTTCCTACCTGGAGAGAGACTTTCCTAATCCGGAGGAC3510.13736102468976366No Hit
CTCCTGGAGAGGGAGGAGTGACTGGGAACTCAGACCAGGTGGATTCCTTG3430.13423028908429896No Hit
GTGTGTTTCTAAGTCTTGTCCACACTAACCTGGGGAAATTTGAAAAAACA3420.13383894713361588No Hit
CGCCAGTTGGCAACCTGCAAGAAACAGCAAGGATAGCATTAATAATAATA3400.1330562632322497No Hit
ACCTAAAACGGCTGGAGGTCGGGGGTCAATGTAATTCTGTGGTAATTTCA3400.1330562632322497No Hit
ATTTAAAACATACGCCATCAATCTTCAAATACACTAAACTTATCCTTTCC3370.13188223738020044No Hit
GTACCTGAGTGGTTTCTTCTCAGTCTTCACGCTCTCAACTGTGTCAAGCT3340.13070821152815118No Hit
ATATGAGGGAGGTGGACTTTGTTTCTCCCTCTCTTCTCTGGAAACATCAT3320.129925527626785No Hit
GAGTTATATCATAGTCTTCATGAACATACCCGAAGCTCCTTTGTTATTAT3310.12953418567610192No Hit
GTCATGTAGTAGGTGCTCATTTGTTAACTGACACAACTGCTAATTCTCAA3290.12875150177473577No Hit
GTCCAATATCAGGCTGTATACTGTTATTGTATTTGCTACAGTTATATGCC3260.12757747592268648No Hit
CTATTGTTTTTGTACCGTTTTCCAAATGTTCTACGTCCAAACGCTTAAAC3240.12679479202132032No Hit
GGATATGATTTCGAATGAGATCCATTTCAATGAAACACTATTTAAGTTAT3240.12679479202132032No Hit
CAGCAAGGATAGCATTAATAATAATAATCATAAATTCAGCCACAGCCTTT3200.12522942421858796No Hit
CCTTTGGATAAGGCAGTGAGGGAGAACAGTGCTGGGAGGTTGTATAGAAT3140.12288137251448943No Hit
GCATTAGATATAGAAAAACAGTCTTAGAAATAACTCCTATGAACCACAAC3090.120924662761074No Hit
GTGTGTATGGAGGGAGATTCTAAATGCCTTAATTAAAATTGTAGGAGTCT3090.120924662761074No Hit
GTTAGAACGTATGAGCTGTGAATAGCAGTCCTCTGAGGCACACAGTGTGT3050.11935929495834165No Hit
GTGTGTGCTTGGGGACCTTGTGTGTTTCTTTGGTGTTGTGAGAGAAATGG3050.11935929495834165No Hit
TCAATTAACTGTGCCGGGGACACGAGTAAAGCAGCCCCTGCTCCTTTGGA3040.11896795300765856No Hit
GTGCTGGGAGGTTGTATAGAATGGAGGTGTCTTCCTACCTGGAGAGAGAC3040.11896795300765856No Hit
GTTTAGTGTATTTGAAGATTGATGGCGTATGTTTTAAATTGAGGTAAAAA3030.11857661105697548No Hit
GTCCTGGAAACACATCAAGCTCAGCTCCTGTGTCCAGCTCGCTTCTCTGC3030.11857661105697548No Hit
CTCTGTCTCTCTCTGTCTTCCTTTGAGCGCCTTTGGCTTGGTAATTTAGC3020.11818526910629239No Hit
GTGAAACACTGTTAGAACGTATGAGCTGTGAATAGCAGTCCTCTGAGGCA3020.11818526910629239No Hit
TGTAGTAGGTGCTCATTTGTTAACTGACACAACTGCTAATTCTCAAAGGG3010.1177939271556093No Hit
GTATACTGTTATTGTATTTGCTACAGTTATATGCCATTATGAGCTTGTCG2990.11701124325424311No Hit
GAGTATAACAGCATTAGATATAGAAAAACAGTCTTAGAAATAACTCCTAT2980.11661990130356004No Hit
CTCCAGATAGGACCAGGAATAAGTTGTCTGGGCTTTTATTTCCCCCCACC2970.11622855935287695No Hit
GGCTGTGACAAAATCACACACGCCAGTTGGCAACCTGCAAGAAACAGCAA2960.11583721740219385No Hit
TTCTTAAACTCTCTAATTTTCCCTGTCCACATTACAACGCAAACCCGGCG2950.11544587545151079No Hit
GTCCTATCTGGAGTGTGAACTAAGTGAAAGAAGAAGGGGGAGGTGTGTAT2940.1150545335008277No Hit
CCTTTGAAAAACGCCGGGTTTGCGTTGTAATGTGGACAGGGAAAATTAGA2890.11309782374741226No Hit
TGCCTACACCGCCTCATAATAATTGATATTCATGACTATTAATATTACAC2880.11270648179672917No Hit
ATTCTATACAACCTCCCAGCACTGTTCTCCCTCACTGCCTTATCCAAAGG2880.11270648179672917No Hit
AGCGAAAGCAGGGGCTGTGACAAAATCACACACGCCAGTTGGCAACCTGC2880.11270648179672917No Hit
CATCAGAGTTATATCATAGTCTTCATGAACATACCCGAAGCTCCTTTGTT2840.11114111399399682No Hit
TTCCTAATCCGGAGGACTTGGTCGCCTTGGGTGCTTTGTGTGAGGATTAC2840.11114111399399682No Hit
ATGCAGAGAGAAGCAGCTGCAGCCTCGTCCAATTATGGTGCTAAATTACC2820.11035843009263063No Hit
GCTCTCTATGGGCTGACATTTTACCTCATTATCTGAAATTGGATACTGGA2820.11035843009263063No Hit
CATCATGGTAAACTCCCCATGGTTCTTTTTGCAGAAATAGTCACCTAAAT2790.10918440424058137No Hit
GTATAGCGAGTGGCGACAAGCTCATAATGGCATATAACTGTAGCAAATAC2760.10801037838853213No Hit
CCTATTCATCCATGGTGAGTTTGGATTCACGTGTTACCAGAATTGCATGC2760.10801037838853213No Hit
GGCTTAGGATATGATTTCGAATGAGATCCATTTCAATGAAACACTATTTA2720.10644501058579978No Hit
CTTTAACACAGCTCTCTATGGGCTGACATTTTACCTCATTATCTGAAATT2700.1056623266844336No Hit
CCCCATGGTTCTTTTTGCAGAAATAGTCACCTAAATTTCCAAACAGCAAT2690.1052709847337505No Hit
CCTCTGAGGCACACAGTGTGTTTCTAAGTCTTGTCCACACTAACCTGGGG2680.10487964278306741No Hit
ATATTACAGGTGGTCCTATCTGCCTAAATTTTCTGCTCAAAAAACAGAAT2670.10448830083238433No Hit
ACTATAGCCTCTCCAGCAGAGCTTTCTATGGGCTGAGAGCCACTGAATGC2670.10448830083238433No Hit
TCCCAGCACTGTTCTCCCTCACTGCCTTATCCAAAGGAGCAGGGGCTGCT2660.10409695888170124No Hit
CAAGGAGGGAGTGAGTCACCTAAAACGGCTGGAGGTCGGGGGTCAATGTA2660.10409695888170124No Hit
GTTATATGCCATTATGAGCTTGTCGCCACTCGCTATACAGCCCAACAAAC2660.10409695888170124No Hit
CTCTCTGTCTCTCTCTGTCTTCCTTTGAGCGCCTTTGGCTTGGTAATTTA2660.10409695888170124No Hit
CCTATGAACCACAACTCAACTTAATTCATCATTGGCTTAGGATATGATTT2650.10370561693101815No Hit
GTTGAGGGCGACATACCCGCAGAATGAAAAACAAGGAGGGAGTGAGTCAC2650.10370561693101815No Hit
GCTTTAACACAGCTCTCTATGGGCTGACATTTTACCTCATTATCTGAAAT2650.10370561693101815No Hit
ATTGTAAATGACGTTCATTCAGTGGAGAATTACTAGATGTAATCAGACGA2620.10253159107896888No Hit
ATCATATCCTAAGCCAATGATGAATTAAGTTGAGTTGTGGTTCATAGGAG2610.1021402491282858No Hit
GGATAAGTTTAGTGTATTTGAAGATTGATGGCGTATGTTTTAAATTGAGG2610.1021402491282858No Hit
GTCCTATCTGCCTAAATTTTCTGCTCAAAAAACAGAATCCGCAGCTCGGT2590.10135756522691965No Hit
GTCTCACACTCTCTGTCTCTCTCTGTCTTCCTTTGAGCGCCTTTGGCTTG2560.10018353937487037No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCGGG100.0070756865145.0829145
CTACAAA100.0070756865145.0829145
AATGTCC151.2261448E-4145.0829145
TGACGCC501.8189894E-12116.06632145
AGCGAAA2150.0114.5371251
GGCTTGT458.54925E-11112.6655961
TGGTTCC203.8539473E-4108.81218145
GACGCTC203.8539473E-4108.81218145
GAGCGAA352.7722126E-7103.468411
TACATTT650.0100.44202145
CCCCGCT259.4085245E-486.930477
GCACTGG259.4085245E-486.930476
CCCGCTC259.4085245E-486.930478
TGTCCTG608.3127816E-1084.515745
GGTGTAA608.3309715E-1084.49921
GCGAAAG3000.082.084942
GCCGACC451.2260698E-680.601616145
CGAAAGC3150.078.176133
GAAAGCA3300.074.6226654
GACACCC607.9760866E-872.54145145
Produced by FastQC (version 0.11.5)