FastQCFastQC Report
Tue 2 Jul 2019
HWC5JBCX2_l02_n02_SM2_9504_P2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l02_n02_SM2_9504_P2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1497936
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCAGACAGATAACTGACACGTTGCTGTTACTGGTTAAA41410.27644705781822454No Hit
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG38930.2598909432712746No Hit
CAACAGCAACGTGTCAGTTATCTGTCTGTTTAATGGCAAAAAGCAATGTG33630.22450892427980904No Hit
CTATAGAACATGCTTGTAAATTCAAACATTCCAGTCTCGTTACAGTAACT29330.19580275792824262No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC29030.19380000213627285No Hit
GTAAGAAATACAGTAAAAATTGAATTTAATGCGATTCTTCTACCTCCAAA27240.18185022591085334No Hit
ATACTATACTGCTCTATTACTCAACTGCTGCTTCTAGTTTGGCTGTAACA25490.17016748379102978No Hit
GTATAGATCTGTTCCTTTTGGTTCTCCAGCTATGAGTTCCAGACACTGCA22630.15107454524091818No Hit
GATATACAGAAAGCACTAATTGGTGCCTCTATCTGCTTTTTAAAACCCAA22280.14873799681695346No Hit
GTAGAAACAACAGCAACGTGTCAGTTATCTGTCTGTTTAATGGCAAAAAG20760.13859070080430674No Hit
CCTTAAGAGTACACAAGAAGCTATAAATAAGATAACCAAAAATCTCAATT19850.13251567490199848No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG18360.12256865446854871No Hit
CTCTTAAGGTCTGCCGCCACTGCCACTCCATGTGCTCCGTGAGATGTGTA18300.12216810331015478No Hit
GAGTGTTCCTAGTTTTACTTGCATTGAATAATTTCCAGGATTCAGGTACA18290.12210134478375578No Hit
ATATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTCAT17660.1178955576206193No Hit
CCCCTAGACCAAATGATGGAAGCATAACAGGGCCTTGCGAATCTGATGGA17570.11729473088302837No Hit
TACCTATACAGGCAGCAATTTCAACAACATTCCCATACACCGGTGTTCCC17390.11609307740784652No Hit
AACAAGAGCAGACAGATAACTGACACGTTGCTGTTACTGGTTAAACAGAC17340.11575928477585158No Hit
CTACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAAT17330.11569252624945257No Hit
GTATTTAGTATAGATCTGTTCCTTTTGGTTCTCCAGCTATGAGTTCCAGA17250.11515845803826065No Hit
TTATTATTGTCATTCCTATTGCCATATCTGCTGATTCATTTACTCCAGCA17200.11482466540626568No Hit
AGAGCAGACAGATAACTGACACGTTGCTGTTACTGGTTAAACAGACAGAT17070.11395680456307879No Hit
GTCTAGGGGTGTCCAAATAAGTCTTTGTGCACATCAATCTTATTTCCGCT16790.11208756582390704No Hit
GTTCTATAGAGATGGATTTGTATCTAACTTTGCAATGGAAATTCCTTCAT16680.11135322203351813No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC16670.11128646350711913No Hit
CCCATACACCGGTGTTCCCCCTTATTCCCATGGAACGGGAACAGGCTACA16050.10714743487038164No Hit
CTGTTAAAACAGGCCTCAAAAAGATTGCAACATTTAGCGTAGCATTGTTC15950.10647984960639172No Hit
GTCTACAGCAGAGGGACCCAGCATTTTCTTTAGTTTTCTCTCAAGTGCCA15930.10634633255359374No Hit
CCATAGAGGCTCTTCATTTGGGTTTTATTATCCGAATGGAACCCCCAAAC15740.10507792055201291No Hit
ATCCAATGATGACCAATAACCCCATGGACATCTTCGGAGCTTATGTGTAC15700.10481088644641694No Hit
CATTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAGCTCTGCTT15660.10454385234082096No Hit
GTAATAAAAGGGTCATTGCCTTTAATTGGTGAAGCAGATTGCCTTCATGA14980.10000427254568953No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCAA100.007072648145.12718145
GCTGCCC500.0145.12718145
GGACGGG202.1216783E-6145.12718145
AACGGCC500.0130.61447145
CGCACCC550.0118.74042145
TGACGCC1050.0117.48392145
GGCACCC800.0108.84539145
GCAGCCC501.9463187E-10101.58903145
GGAGCGT406.152841E-790.5409853
GGACCCC259.3550247E-487.07631145
CGGTGCA750.086.9135362
GGTTGCC353.4229175E-582.929825145
GCACGCA2550.079.528732
AGCACGC2600.077.999321
GGTGCAT851.8189894E-1276.693543
CACGCAC2650.076.532753
AACACGT4550.076.425366
AGACGCC406.635382E-572.56359145
GCCGACC502.2948152E-672.56359145
TCTCGGG406.635382E-572.56359145
Produced by FastQC (version 0.11.5)