FastQCFastQC Report
Thu 11 Jul 2019
HWC5JBCX2_l02_n02_SM2_9504_P2_DM2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l02_n02_SM2_9504_P2_DM2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1506916
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCAGACAGATAACTGACACGTTGCTGTTACTGGTTAAA41530.2755959854431169No Hit
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG39120.25960305683926643No Hit
CAACAGCAACGTGTCAGTTATCTGTCTGTTTAATGGCAAAAAGCAATGTG33770.22410008255271033No Hit
CTATAGAACATGCTTGTAAATTCAAACATTCCAGTCTCGTTACAGTAACT29430.19529953892585916No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC29110.1931759965386259No Hit
GTAAGAAATACAGTAAAAATTGAATTTAATGCGATTCTTCTACCTCCAAA27330.1813637920096409No Hit
ATACTATACTGCTCTATTACTCAACTGCTGCTTCTAGTTTGGCTGTAACA25630.17008247307746416No Hit
GTATAGATCTGTTCCTTTTGGTTCTCCAGCTATGAGTTCCAGACACTGCA22710.15070514879396063No Hit
GATATACAGAAAGCACTAATTGGTGCCTCTATCTGCTTTTTAAAACCCAA22350.14831616360832323No Hit
GTAGAAACAACAGCAACGTGTCAGTTATCTGTCTGTTTAATGGCAAAAAG20830.13822933726896522No Hit
CCTTAAGAGTACACAAGAAGCTATAAATAAGATAACCAAAAATCTCAATT19980.13258867780287686No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG18420.1222364086651147No Hit
GAGTGTTCCTAGTTTTACTTGCATTGAATAATTTCCAGGATTCAGGTACA18350.12177188376790743No Hit
CTCTTAAGGTCTGCCGCCACTGCCACTCCATGTGCTCCGTGAGATGTGTA18340.12170552306830638No Hit
ATATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTCAT17730.11765752039264298No Hit
CCCCTAGACCAAATGATGGAAGCATAACAGGGCCTTGCGAATCTGATGGA17640.11706027409623364No Hit
TACCTATACAGGCAGCAATTTCAACAACATTCCCATACACCGGTGTTCCC17420.11560033870501077No Hit
AACAAGAGCAGACAGATAACTGACACGTTGCTGTTACTGGTTAAACAGAC17410.11553397800540972No Hit
CTACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAAT17370.11526853520700557No Hit
GTATTTAGTATAGATCTGTTCCTTTTGGTTCTCCAGCTATGAGTTCCAGA17270.11460492821099516No Hit
TTATTATTGTCATTCCTATTGCCATATCTGCTGATTCATTTACTCCAGCA17240.11440584611219205No Hit
AGAGCAGACAGATAACTGACACGTTGCTGTTACTGGTTAAACAGACAGAT17100.1134767963177775No Hit
GTCTAGGGGTGTCCAAATAAGTCTTTGTGCACATCAATCTTATTTCCGCT16820.11161869672894839No Hit
GTAGTAACAAGAGGATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC16750.11115417183174112No Hit
GTTCTATAGAGATGGATTTGTATCTAACTTTGCAATGGAAATTCCTTCAT16730.11102145043253905No Hit
CCCATACACCGGTGTTCCCCCTTATTCCCATGGAACGGGAACAGGCTACA16120.10697344775687564No Hit
GTCTACAGCAGAGGGACCCAGCATTTTCTTTAGTTTTCTCTCAAGTGCCA16020.10630984076086523No Hit
CTGTTAAAACAGGCCTCAAAAAGATTGCAACATTTAGCGTAGCATTGTTC15980.10604439796246107No Hit
CCATAGAGGCTCTTCATTTGGGTTTTATTATCCGAATGGAACCCCCAAAC15800.10484990536964237No Hit
ATCCAATGATGACCAATAACCCCATGGACATCTTCGGAGCTTATGTGTAC15740.10445174117203614No Hit
CATTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAGCTCTGCTT15720.10431901977283405No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACGGG202.1226842E-6145.11342145
CGCACCC550.0131.92128145
GCAGCCC458.367351E-11112.86599145
TGACGCC850.0110.969086145
CTTGGCC404.791218E-9108.83506145
GGCACCC301.593524E-596.74227145
CGGTGCA900.096.5753562
AGCACGC2500.084.017761
GCGAGAA353.4245364E-582.92195145
GCACGCA2550.082.373092
CACGCAC2600.080.7893
GCTGCCC900.080.61856145
GGTGCAT1150.075.580713
CGTGCTA451.1996511E-464.383573
ACGTGCA2600.064.0740362
AACACGT3400.061.785976
GCGGTGC2250.061.1623571
CACCCCC851.2978489E-859.752583145
ACACGTG3450.058.7927977
TAGTAAC29550.057.847162
Produced by FastQC (version 0.11.5)