FastQCFastQC Report
Tue 2 Jul 2019
HWC5JBCX2_l02_n02_SM2_9497_P2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l02_n02_SM2_9497_P2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1424848
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTATGCACCGCGCACGCGACCGAGTAAGGCCACTCAATGACATCCATTT65000.4561890110383704No Hit
GAGTATGCACCGCGCACGCGACCGAGTAAGGCCACTCAATGACATCCATT41760.29308389386095923No Hit
ATTATTATGTGGTCATTCAATACCTCCATGTTGTCAGAGAGTACTTCCTT40130.28164407712261236No Hit
GTACACATGTGCGTGTTTGGCCTCCTCATCTTCATTGTCTACGAGGCACT39650.27827529673340595No Hit
CTACTATACTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAAT38190.2680285897162364No Hit
GGTATATGCCATCTACACGCTTTTTTGGTGCACCTCTCACGACAACTCCC37490.263115784981977No Hit
GTAAGAAATACAGTAAAATTGAATTTAATGCAATTCTTCTATCTCCAAAA37250.2614313947873738No Hit
TCTCACGACAACTCCCGCCTCGACAAATATTAAAGCGCTTCGCTTCCGAA35370.24823700492964865No Hit
GTGCATACTCTCGGGGCGGTGACTGCTGTGAGCACGTGCGATCTCGGATG32950.23125273713406622No Hit
CTATTGTCTTGCTCTGGTTTGCAGAGCAATTCCTCTCTGTCCGTAGAGGT28990.20346029892311318No Hit
CCCCGAGAGTATGCACCGCGCACGCGACCGAGTAAGGCCACTCAATGACA28170.19770529909155224No Hit
ATATTAAAGCGCTTCGCTTCCGAACAGACATAAAATGGACACGCAAGTCG27660.1941259699280204No Hit
GTCTACGAGGCACTATTGTCTTGCTCTGGTTTGCAGAGCAATTCCTCTCT27070.1899851773662875No Hit
CACGTGCTCACAGCAGTCACCGCCCCGAGAGTATGCACCGCGCACGCGAC25910.18184395809237194No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT25910.18184395809237194No Hit
GTTCACAGGAGACTGGGAAGAATATCCCGTACGAAACCCTTTACCCGAAG23860.16745645851346952No Hit
GACATAAAATGGACACGCAAGTCGGGTCAGCCCCGACCGCCTTAACGATT23680.1661931658675171No Hit
GTCCGTAGAGGTCCCACTTGCAGATGAGTACGGCCAAGGCCTTGCTTGGA23450.164578958597689No Hit
CTTTTTTGGTGCACCTCTCACGACAACTCCCGCCTCGACAAATATTAAAG23250.16317530010218634No Hit
TATATATGCCGGGTCATTGCAAAAACGGATCCTCGGTTCACAGGAGACTG22810.16008725141208044No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG22730.15952578801387937No Hit
GCATAAAGGCGGGTGTCCTGCTTTCTTATTTCATCGTTGTCCCACTCTCC22710.1593854221643291No Hit
GTACTTCCTTCATTGTTTCTTTGGCCTGGATTTCTTTTTGGTAACTGTGT21910.1537707881823184No Hit
CAATTATTATGTGGTCATTCAATACCTCCATGTTGTCAGAGAGTACTTCC20240.14205023974487105No Hit
GTAGTAACAAGAGCCGGGAAAGGTTGCATAAAGGCGGGTGTCCTGCTTTC20040.14064658124936835No Hit
TTTCATGTCTGTTCGGAAGCGAAGCGCTTTAATATTTGTCGAGGCGGGAG19990.14029566662549267No Hit
CACGAGCTCACAGCAGTCACCGCCCCGAGAGTATGCACCGCGCACGCGAC19880.13952365445296622No Hit
GGCATATGCCATCTACACGCTTTTTTGGTGCACCTCTCACGACAACTCCC19850.13931310567864083No Hit
GTTTAGTATACCGTTAGAAAGATATAATGAAGAAACAAGGGCAAAATTGA19320.13559341066555872No Hit
GAGTAAGGCCACTCAATGACATCCATTTCCCAGGTATATGCCATCTACAC18970.13313700829842903No Hit
CGTATATATGCCGGGTCATTGCAAAAACGGATCCTCGGTTCACAGGAGAC18740.13152280102860095No Hit
CTTTAATATTTGTCGAGGCGGGAGTTGTCGTGAGAGGTGCACCAAAAAAG18720.13138243517905068No Hit
GCCTTGTACACATGTGCGTGTTTGGCCTCCTCATCTTCATTGTCTACGAG17600.12352194760423568No Hit
CATTCACACAGGGCTCATAGCAGAGCAGCGAGATCTTCAGTGCCTGGAGT17570.12331139882991028No Hit
GTATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTCAT17460.1225393866573838No Hit
CTGTAGAACATGCTTGTAAATTCAAACATTCCAGTCTCATTACAGTAACT17140.12029353306457953No Hit
GTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAGTAAAA16600.1165036551267223No Hit
TATCTATAATGCTCGAACCATTTCAGATTCTTTCAATTTGTTCTTTTATC16260.11411743568436775No Hit
CTCTCCACCCTGAGCTTTTCTTAGTGTTTTAAGCGTGGGTTGGAGAGCGA16010.11236286256498938No Hit
GAATATCCCGTACGAAACCCTTTACCCGAAGACATCCGAGATCGCACGTG15480.10864316755190728No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA15410.10815188707848135No Hit
GTTTAGACGCGAAAATTGTTCACGCTCTGCCGAGGCGCTTACGGCCAAGG15100.1059762164104522No Hit
GCTCACAGCAGTCACCGCCCCGAGAGTATGCACCGCGCACGCGACCGAGT14720.10330926526899711No Hit
CGTCTAAACAGATCGAATACGTATATATGCCGGGTCATTGCAAAAACGGA14500.10176524092394418No Hit
ACTATACTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGC14280.10022121657889123No Hit
GCTATGAACACAGCAAAAACAATGAATGGAATGGGAAAAGGAGAAGACGT14260.10008085072934095No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACCCC1200.0102.78112145
CTTCGCC1150.088.32342145
GCGACCC502.2582753E-887.06165145
AGCACGG750.087.06165145
AGCACGC4250.083.5271151
GCACGCA4350.079.9415052
TCGCTCC554.3743057E-879.14696145
TTGCGCC451.1898525E-464.49012145
CGGGGCC808.043571E-963.482456145
ACGCTAC350.003556817862.186897145
CTGTAAA606.7785368E-660.45948145
CTACACC1200.060.45948145
TGACGCC902.0389052E-856.428852145
TGCACCC1850.054.903748145
CCATGCC400.00603447454.413532145
CATTGCC1004.6789864E-850.785965145
GCTATCC450.00961302548.367588145
GACCGAA450.00961302548.367588145
GTTGCCC450.00961302548.367588145
GTTACGG450.00966786248.2979971
Produced by FastQC (version 0.11.5)