FastQCFastQC Report
Thu 11 Jul 2019
HWC5JBCX2_l02_n02_SM2_9497_P2_DM2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l02_n02_SM2_9497_P2_DM2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1433504
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTATGCACCGCGCACGCGACCGAGTAAGGCCACTCAATGACATCCATTT65210.4548993236154207No Hit
GAGTATGCACCGCGCACGCGACCGAGTAAGGCCACTCAATGACATCCATT41950.29263957407862135No Hit
ATTATTATGTGGTCATTCAATACCTCCATGTTGTCAGAGAGTACTTCCTT40190.28036196620309395No Hit
GTACACATGTGCGTGTTTGGCCTCCTCATCTTCATTGTCTACGAGGCACT39810.27771111904815055No Hit
CTACTATACTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAAT38280.2670379712927205No Hit
GGTATATGCCATCTACACGCTTTTTTGGTGCACCTCTCACGACAACTCCC37600.26229435006808494No Hit
GTAAGAAATACAGTAAAATTGAATTTAATGCAATTCTTCTATCTCCAAAA37360.2606201308123312No Hit
TCTCACGACAACTCCCGCCTCGACAAATATTAAAGCGCTTCGCTTCCGAA35420.24708685849498851No Hit
GTGCATACTCTCGGGGCGGTGACTGCTGTGAGCACGTGCGATCTCGGATG33080.23076322075138958No Hit
CTATTGTCTTGCTCTGGTTTGCAGAGCAATTCCTCTCTGTCCGTAGAGGT29160.2034176395740786No Hit
CCCCGAGAGTATGCACCGCGCACGCGACCGAGTAAGGCCACTCAATGACA28280.1972788356363149No Hit
ATATTAAAGCGCTTCGCTTCCGAACAGACATAAAATGGACACGCAAGTCG27780.19379087885349464No Hit
GTCTACGAGGCACTATTGTCTTGCTCTGGTTTGCAGAGCAATTCCTCTCT27120.18918677590017188No Hit
CACGTGCTCACAGCAGTCACCGCCCCGAGAGTATGCACCGCGCACGCGAC26050.18172254838493648No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT25980.18123423443534165No Hit
GTTCACAGGAGACTGGGAAGAATATCCCGTACGAAACCCTTTACCCGAAG23940.1670033707614349No Hit
GACATAAAATGGACACGCAAGTCGGGTCAGCCCCGACCGCCTTAACGATT23720.165468669776994No Hit
GTCCGTAGAGGTCCCACTTGCAGATGAGTACGGCCAAGGCCTTGCTTGGA23570.1644222827421479No Hit
CTTTTTTGGTGCACCTCTCACGACAACTCCCGCCTCGACAAATATTAAAG23310.16260854521508136No Hit
TATATATGCCGGGTCATTGCAAAAACGGATCCTCGGTTCACAGGAGACTG22940.16002745719579436No Hit
GCATAAAGGCGGGTGTCCTGCTTTCTTATTTCATCGTTGTCCCACTCTCC22820.15919034756791747No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG22780.15891131102529185No Hit
GTACTTCCTTCATTGTTTCTTTGGCCTGGATTTCTTTTTGGTAACTGTGT22030.15367937585106145No Hit
CAATTATTATGTGGTCATTCAATACCTCCATGTTGTCAGAGAGTACTTCC20320.1417505636538161No Hit
GTAGTAACAAGAGCCGGGAAAGGTTGCATAAAGGCGGGTGTCCTGCTTTC20080.14007634439806238No Hit
TTTCATGTCTGTTCGGAAGCGAAGCGCTTTAATATTTGTCGAGGCGGGAG20030.13972754871978035No Hit
CACGAGCTCACAGCAGTCACCGCCCCGAGAGTATGCACCGCGCACGCGAC19970.1393089939058419No Hit
GGCATATGCCATCTACACGCTTTTTTGGTGCACCTCTCACGACAACTCCC19900.13882067995624706No Hit
GTTTAGTATACCGTTAGAAAGATATAATGAAGAAACAAGGGCAAAATTGA19420.1354722414447396No Hit
GAGTAAGGCCACTCAATGACATCCATTTCCCAGGTATATGCCATCTACAC19040.1328213942897962No Hit
CTTTAATATTTGTCGAGGCGGGAGTTGTCGTGAGAGGTGCACCAAAAAAG18820.13128669330535525No Hit
CGTATATATGCCGGGTCATTGCAAAAACGGATCCTCGGTTCACAGGAGAC18780.13100765676272963No Hit
GCCTTGTACACATGTGCGTGTTTGGCCTCCTCATCTTCATTGTCTACGAG17710.12354342924749426No Hit
CATTCACACAGGGCTCATAGCAGAGCAGCGAGATCTTCAGTGCCTGGAGT17660.12319463356921222No Hit
GTATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTCAT17530.12228776480567895No Hit
CTGTAGAACATGCTTGTAAATTCAAACATTCCAGTCTCATTACAGTAACT17190.11991595419336117No Hit
GTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAGTAAAA16650.11614896086791526No Hit
TATCTATAATGCTCGAACCATTTCAGATTCTTTCAATTTGTTCTTTTATC16330.11391666852691028No Hit
CTCTCCACCCTGAGCTTTTCTTAGTGTTTTAAGCGTGGGTTGGAGAGCGA16060.11203317186418732No Hit
GAATATCCCGTACGAAACCCTTTACCCGAAGACATCCGAGATCGCACGTG15520.10826617853874143No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA15450.1077778645891466No Hit
GTTTAGACGCGAAAATTGTTCACGCTCTGCCGAGGCGCTTACGGCCAAGG15170.10582460879076723No Hit
GCTCACAGCAGTCACCGCCCCGAGAGTATGCACCGCGCACGCGACCGAGT14790.10317376163582384No Hit
CGTCTAAACAGATCGAATACGTATATATGCCGGGTCATTGCAAAAACGGA14500.10115074670178806No Hit
ACTATACTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGC14340.10003460053128557No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGACCC301.595169E-596.721855145
CTTCGCC850.093.87709145
AGCACGC4900.082.785891
TGTGCGG451.228982E-680.60155145
CTACACC1350.080.60155145
GCACGCA5000.078.2326662
TTACCCC1200.072.54139145
CGGGGCC902.8012437E-1064.48124145
AGACGGG451.19066615E-464.48124145
AGCACGG954.5474735E-1061.087486145
GTTCGGG400.0060377754.40604145
ATTGCGG400.0060377754.40604145
CCGACCC701.6906364E-551.81528145
GCATACC701.6906364E-551.81528145
AATGGCC858.892457E-751.20569145
ACGGGAA901.3187819E-648.360928145
TCGCTCC604.931901E-448.360928145
TGCCACG1551.4551915E-1146.8009145
ACTCATT657.317048E-444.640858145
CGGTGCG1307.410563E-944.577022
Produced by FastQC (version 0.11.5)