FastQCFastQC Report
Tue 2 Jul 2019
HWC5JBCX2_l02_n02_SM2_9464_P2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l02_n02_SM2_9464_P2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1844746
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCCAACACTGTGTCAAGTTTCCAGGTAGATTGCTTTCTTTGGCATATCC100690.5458204002068577No Hit
CTTTTAAGGCCGTGTTTGAAGAGTCGATAGACGCATTTGAAAAAAAGACG71800.38921347437533405No Hit
GTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCCTGCACCATCTG57300.31061186743324015No Hit
GAATCACAGTATCTACCAAAAGAAGCCAACAAGCTGTAATCCCAAATATC48960.2654023914403392No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT46350.2512541021907623No Hit
TTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATC44190.2395451731566297No Hit
GTATACTAGAGTCCCGTTTTCGTTTCATTACCAACACCACGTCTCCTTGC41690.22599317195971694No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCACGGGGCCAAAGAAATAG41590.22545109191184046No Hit
GTATTATTATCTCCTGAGGAGGTCAGTGAGACACAGGGAACTGAAAGACT36480.1977508014653508No Hit
GTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG35980.19504040122596825No Hit
GGGTGACAAAGACATAATGGATTCCAACACTGTGTCAAGTTTCCAGGTAG34930.1893485607232649No Hit
CTTCTGCATTGTCTCCGAAGAAATAAGATCCTTCGTTACTCATATCAAAA33240.18018740791415186No Hit
CAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGTGTG32770.17763963168913227No Hit
TATCAAAAGAGGGCACGATCGGGTTCGCTGCCTTCTCGTCTGAGAGCTCG31330.16983367899971052No Hit
CTCATAGACTCAGGTACTCCTTCCGTAGAAGGGCCTCTTTTAAGGCCGTG30630.16603911866457496No Hit
TAGTAATACTATGGTCTCTAGTCGGCCAAAAATCACATTGAAATTCGCTT29840.1617566862863505No Hit
CTCATGGAATGGCTAAAGACAAGACCAATTCTGTCACCTTTGACTAAGGG29480.1598051981139951No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT29400.15937153407569388No Hit
GTATATACATTGAAGTTTTACATTTGACTCAAGGAACGTGTTGGGAACAA28780.1560106377788595No Hit
GTTGAGACCATGGTCATTTTAAGTGTCTCATCAGATTCTCCTTTCAGAAT28770.1559564297740719No Hit
CAAGTTGTAGACCAAAAACTGAGTGATGCCCCATTCCTCGATAGGCTTCG28640.15525172571183243No Hit
CCTCAATAGTATGTCCTGGAAAAGAAGGCAATGGTGAGATTTCGCCAACA28100.15232449345329926No Hit
CTCCAGTACAGAGAGGGTGGTGGTTAGCATTGATCGGTTTTTGAGAGTTC27470.14890938915167726No Hit
GTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATCTC26010.1409950204526802No Hit
ATCCTATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATA25590.13871828425159888No Hit
GTATTGGCTTCTAGTGGCCTTGGGGACTAAAGATTGAAATGGTTCAAATT25570.13860986824202357No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCC25290.13709204410796932No Hit
GTACTGGAGTATTCATCCACACCCATTTTGCTAACTCTTATTCCTCTCAT24100.13064129153823886No Hit
CTCTTAGACCAGTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCC23900.12955713144248585No Hit
CTATTGAGGATGTCAAAAATGCAATTGGGGTCCTCATCGGAGGACTTGAA23890.1295029234376982No Hit
GGTGGCTTTACATTTAAAAGAACAAGCGGGTCGTCAGTCAAAAAAGAAGA23740.12868980336588343No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC23410.12690093920789094No Hit
TTCTTAATCTGTGTCTCACCTCTTCAATTAGCCATCTTATCTCTTCAAAC22770.12343162690148128No Hit
GTGTTATCATTCCATTCAAGTCCTCCGATGAGGACCCCAATTGCATTTTT22740.12326900288711833No Hit
GACATAATGGATTCCAACACTGTGTCAAGTTTCCAGGTAGATTGCTTTCT22060.11958285856155806No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA21620.11719770635090143No Hit
GTGTCAAGTTTCCAGGTAGATTGCTTTCTTTGGCATATCCGGAAACAAGT21310.11551725820248425No Hit
GGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATCAGAAACGAAT21010.11389101805885471No Hit
TTCTTAAACAGAACCCGACTGAAGAACAAGCTGTGGATATATGCAAGGCT20920.11340314601576584No Hit
TGTTTGAAGAGTCGATAGACGCATTTGAAAAAAAGACGATCAAGAATCCA20640.11188532188171163No Hit
CTCCTGAGGAGGTCAGTGAGACACAGGGAACTGAAAGACTGACAATAACT20600.11166848986256103No Hit
ATATACAGGAGAGTCGATGGGAAATGGATGAGGGAACTCGTCCTTTATGA20480.11101799380510921No Hit
TCCTAGTTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGA20440.11080116178595861No Hit
CATTAACACTGCCCTGCTCAATGCATCCTGTGCAGCAATGGACGATTTTC20260.1098254176997809No Hit
ATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT20230.10966279368541793No Hit
TAATAATACATTCCCACGTTGGTCTCGAACTCTCAAAAACCGATCAATGC19930.10803655354178841No Hit
CCCTAGAAGGTCCGGGGCTGCAGGTGCTGCAGTCAAAGGAATCGGAACAA19870.10771130551306252No Hit
ATTATATTCAGCATGGAAAGAATAAAAGAACTACGGAATCTAATGTCGCA19760.10711501746039835No Hit
GTTGTAATGCTTGCATGAATGTTATTTGTTCAAAGCTATTTTCAGTTGTT19380.10505511327846762No Hit
TTACTAAGGAGTGCCATAGGCCAAATTTCAAGGCCGATGTTCTTGTATGT19350.10489248926410465No Hit
GACTTGAAGATGTCTTTGCTGGGAAAAACACAGATCTTGAGGCTCTCATG19150.10380832916835163No Hit
CCTTAGTAACATATCTCCTATCTCAAGGACACAATATTTCTCCCATTTAT19100.10353728914441337No Hit
ATCCCATATTATCCATGTTCTCATTTGAAGCGATTTGTACTCCTCTAGTT19010.10304941710132451No Hit
CTGCTGTTCCTTTCGATATTCTTCCCTCATAGACTCAGGTACTCCTTCCG18650.10109792892896909No Hit
CTCTTATTCCTCTCATTGACATCTCTGTACTTGGAGTCATATCTGGTAAT18570.10066426489066788No Hit
CATTTTAAGTGTCTCATCAGATTCTCCTTTCAGAATCTTTTCTACGATTT18480.10017639284757901No Hit
GCACTACAGCTAAGGCTATGGAGCAAATGGCTGGATCAAGTGAGCAGGCA18460.10006797683800371No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACC100.007078177145.09016145
GCTGACC1500.0116.072136145
AGCGAAA4350.0114.881251
TCACGCC458.367351E-11112.8479145
TGACGCC458.367351E-11112.8479145
GAAGCCC1200.0108.81763145
ACCGACC203.8570302E-4108.81762145
CGAAAGC5450.093.023113
GAGCGAA800.090.531421
AGACGCC554.37758E-879.14008145
CCTCGCC851.8189894E-1276.81244145
AGCGTAA406.644183E-572.54508145
GGTCTTT608.000825E-872.54508145
CGACTAA300.001931233772.54508145
TTATATT3900.068.711032
TATATTC4000.066.993253
GTGGGCC1300.066.96469145
CAGGGGG651.3927092E-766.96469145
GCCCGGG451.1904568E-464.48451145
ATCGACC451.1904568E-464.48451145
Produced by FastQC (version 0.11.5)