FastQCFastQC Report
Tue 2 Jul 2019
HWC5JBCX2_l02_n02_SM2_9459_P2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l02_n02_SM2_9459_P2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1736003
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTTAAGGCCGTGTTTGAAGAGTCGATAGACACATTTGAAAAAAAGACG86850.5002871538816466No Hit
GTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCCTGCACCATCTG63510.36584038161224375No Hit
ACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCTGCTGT60580.3489625305947052No Hit
TTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATC59010.33991876742148486No Hit
TTCCAACACTGTGTCAAGCTTCCAGGTAGATTGCTTTCTTTGGCATATCC51480.2965432663422817No Hit
GTATAGGAAACTTAAGAGGGAGATAACGTTCCACGGGGCCAAAGAAATAG46070.26537972572628044No Hit
CTCCAACTCTATGCTGACAAAATGACTGTCGTCAGCATCCACAGCATTCT39960.23018393401393894No Hit
CAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGTGTG38650.22263786410507358No Hit
CTCATAGACTCAGGTACTCCTTCCGTAGAAGGGCCTCTTTTAAGGCCGTG36650.2111171466869585No Hit
TAGTAATACTATGGTCTCTAGTCGGCCAAAAATCACATTGAAATTCGCTT35030.2017853655782853No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGGTCTCCATTCCC34020.19596740328213716No Hit
GTATACTAGAGTCCCGTTTTCGTTTCATTACCAACACCACGTCTCCTTGC33240.1914743234890723No Hit
CCTCAATAGTATGTCCTGGAAAAGAAGGCAATGGTGAGATTTCGCCAACA31780.18306419977384833No Hit
GTATTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG31460.1812208849869499No Hit
ATCCTATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATA31300.1802992275935007No Hit
TTCTAACCGAGGTCGAAACGTATGTTCTCTCTATCGTTCCATCAGGCCCC30490.17563333703916412No Hit
GGTCTAAGAGATGATCTTCTTGAAAATTTGCAGACCTATCAGAAACGAAT29800.1716586895299144No Hit
CTCATGGAATGGCTAAAGACAAGACCAATTCTGTCACCTTTGACTAAGGG29660.17085223931064636No Hit
TCCTAGTTCCAGTACTGGTCTAAGAGATGATCTTCTTGAAAATTTGCAGA29500.16993058191719715No Hit
CTCTTAGACCAGTACTGGAACTAGGATGAGTCCCAATGGCTCTCATTGCC29150.167914456369027No Hit
ATGCCCAAGCAGAAAGTGGAAGGACCTCTTTGCATCAGAATGGACCAGGC27720.15967714341507475No Hit
TTCTTAATCTGTGTCTCACCTCTTCAATTAGCCATCTTATCTCTTCAAAC27530.1585826752603538No Hit
GTGTTATCATTCCATTCAAGTCCTCCGATGAGGACCCCAATTGCATTTTT27040.15576009949291564No Hit
GACTTGAAGATGTCTTTGCTGGGAAAAACACAGATCTTGAGGCTCTCATG26360.1518430555707565No Hit
GTATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAAATCTC25960.1495389120871335No Hit
CTATTGAGGATGTCAAAAATGCAATTGGGGTCCTCATCGGAGGACTTGAA25560.14723476860351048No Hit
ATTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGA24990.1439513641393477No Hit
TGTTTGAAGAGTCGATAGACACATTTGAAAAAAAGACGATCAAGAATCCA24590.1416472206557247No Hit
GCACTACAGCTAAGGCTATGGAGCAAATGGCTGGATCAAGTGAGCAGGCA23920.13778778032065614No Hit
CTGCTGTTCCTTTCGATATTCTTCCCTCATAGACTCAGGTACTCCTTCCG23420.13490760096612736No Hit
ATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGT23310.13427396150813103No Hit
ATTATATTCAGCATGGAAAGAATAAAAGAACTACGGAATCTAATGTCGCA23280.1341011507468593No Hit
GCATTCTGCTGTTCCTTTCGATATTCTTCCCTCATAGACTCAGGTACTCC22700.13076014269560596No Hit
GTTGTAATGCTTGCATGAATGTTATTTGTTCAAAGCTATTTTCAGTTGTT22150.1275919454056243No Hit
CTTCTGCATTGTCTCCGAAGAAATAAGATCCTTCGTTACTCATATCAAAA21360.12304126202546885No Hit
GTATATGAGGCCCATGCAACTGGCAAGTGCACCAGCAGAATAACTGAGAG21360.12304126202546885No Hit
GCAGTTAAACTGTATAGGAAACTTAAGAGGGAGATAACGTTCCACGGGGC21210.12217720821911021No Hit
ATATACAGGAGAGTCGATGGGAAATGGATGAGGGAACTCGTCCTTTATGA20940.12062191136766467No Hit
TATCAAAAGAGGGCACGATCGGGTTCGCTGCCTTCTCGTCTGAGAGCTCG20720.11935463245167204No Hit
GGCCTGGTGTGTGCAACATGTGAGCAGATTGCTGATTCCCAGCACAGGTC20610.1187209929936757No Hit
CAGGTACTCCTTCCGTAGAAGGGCCTCTTTTAAGGCCGTGTTTGAAGAGT20100.11578321005205636No Hit
AAACAAGGTAGTTTTTTACTCCAACTCTATGCTGACAAAATGACTGTCGT20060.11555279570369405No Hit
GTATATACATTGAAGTTTTACATTTGACTCAAGGAACGTGTTGGGAACAA20060.11555279570369405No Hit
TCTCTCTATCGTTCCATCAGGCCCCCTCAAAGCCGAGATCGCGCAGAGAC19760.1138246880909768No Hit
GTCCAAAATGCCCTCAATGGGAATGGAGACCCAAATAACATGGACAAAGC19740.11370948091679565No Hit
ATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGGTGT19360.11152054460735379No Hit
CACCAGGCCAAATGCCACTTCAGTGGTTACAGCCCCCATCCTATTGTATA18770.10812193296900983No Hit
GTCCCAATGGCTCTCATTGCCTGCACCATCTGCCTGGCCTGACTAGCAAT18400.10599060024665856No Hit
GCCTTAGCTGTAGTGCTGGCCAAAACCATTCTGTTCTCATGTTTTATTAA18380.10587539307247741No Hit
AGTAGAAACAAGGTAGTTTTTTACTCCAACTCTATGCTGACAAAATGACT18130.10443530339521302No Hit
GGTAGATATTGAAAGATGAGCCTTCTAACCGAGGTCGAAACGTATGTTCT18130.10443530339521302No Hit
CATATACAATAGGATGGGGGCTGTAACCACTGAAGTGGCATTTGGCCTGG18000.10368645676303555No Hit
CCCTAGAAGGTCCGGGGCTGCAGGTGCTGCAGTCAAAGGAATCGGAACAA17780.1024191778470429No Hit
CGTATGTTCTCTCTATCGTTCCATCAGGCCCCCTCAAAGCCGAGATCGCG17730.10213115991159001No Hit
CCCAAATAACATGGACAAAGCAGTTAAACTGTATAGGAAACTTAAGAGGG17500.10080627740850678No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGTAA100.0070741926145.11723145
TTCGAGG351.891749E-9124.38621145
TCGACCC203.854129E-4108.83793145
GAAGCCC750.0106.41931145
GGCAGCC352.7493297E-7103.655174145
AGCGTAA501.9463187E-10101.58207145
TGACGCC501.9463187E-10101.58207145
CAGGGGG451.0859367E-896.74483145
AACCGCC259.357825E-487.07034145
CGACGAA502.257002E-887.07034145
TCTCGGG502.257002E-887.07034145
AGCGAAA3350.084.313091
GCTGACC950.084.01524145
TGTGCCC353.4242345E-582.92414145
CGACTCC353.4242345E-582.92414145
TCACGCC801.8189894E-1281.62845145
AACTGCC451.2273558E-680.62069145
AGACGCC651.5552359E-978.14005145
GCAATCC1350.075.24598145
AATTCTA1650.074.621715
Produced by FastQC (version 0.11.5)