FastQCFastQC Report
Tue 2 Jul 2019
HWC5JBCX2_l02_n02_SM2_9443_P2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHWC5JBCX2_l02_n02_SM2_9443_P2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1676038
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATATACAGAAAGCACTAATTGGTGCCTCTATCTGCTTTTTAAAACCCAA62270.3715309557420536No Hit
GTCTAATTGTCTCCCTCTTCTGGTGATAATCGGTGCTCTTGACCAAATTG62140.37075531700355246No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT57650.3439659482660894No Hit
ATATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTCAT52180.3113294567306947No Hit
CATTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAGCTCTGCTT48140.2872249913188126No Hit
GTAAGAAATACAGTAAAAATTGAATTTAATGCGATTCTTCTACCTCCAAA44920.2680130164113224No Hit
CTACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAAT43040.2567960869622288No Hit
GTATTAAGGGACATGAACAACAAAGATGCAAGGCAAAAGATAAAGGAGGA39070.23310927317877042No Hit
GCTTTAGCACTTCCATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGA38640.23054369888988197No Hit
GTTCATAGCTGAGACCATCTGCATTTCCCGTCTCACTCCTGGCACTGAGG35020.2089451432485421No Hit
GCCTTGGAATGGATAAAAAACAAAAGATGCTTAACTGATATACAGAAAGC34200.2040526527441502No Hit
ACTATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGC33830.2018450655653392No Hit
GTGTGAATGTGATGCTTGTTTTTCGCACAAAGCACAGAGTGTTCCTAGTT33460.1996374783865282No Hit
GTATAGTATGGTTATCCAATCCATCATCATTTAAAGATGCAGCAGTAATG32440.19355169751521145No Hit
GTATGAAAAGTTCCTCAGAGAGCAACTGTCCGAAATACAGTTGGATTGAT31830.18991216189609064No Hit
CTTTTCATACAGTATGGCTCAAACCCTTCAATTCCAGCAGATGGATTCAT31580.1884205489374346No Hit
GGTCAAGACCGTCTAAACAGACTAAAGAGGAAATTAGAGTCAAGAATAAA31480.18782390375397218No Hit
ATATTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAG31240.18639195531366234No Hit
GTCCTATTGTCCAAGCCATGAAATGGAGAGCTGATAAAATAAAAGAACAA30670.1829910777679265No Hit
GCTATGAGCTCTGTGTGAATGTGATGCTTGTTTTTCGCACAAAGCACAGA27880.16634467714932477No Hit
CAATATGACCACAACACAAATTGAGGTGGGTCCGGGAGCAACCAATGCCA27290.16282447056689645No Hit
AGCAGGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT27090.1616311801999716No Hit
CTTCTGACTTGCCCCAAGGGATCTCAATACTCCAATGTTGCTTTGCAGTT26950.1607958769431242No Hit
CATTCACACAGAGCTCATAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGT25180.15023525719583924No Hit
ATTACATCCTTTGCAATTCCTTCCCCATTCTTCTGACTTGCCCCAAGGGA24530.14635706350333344No Hit
GTATGGCTCAAACCCTTCAATTCCAGCAGATGGATTCATAAATAAAAGTA24370.14540243120979357No Hit
GCACTAATTGGTGCCTCTATCTGCTTTTTAAAACCCAAAGACCAGGAAAG24150.14408981180617622No Hit
GAGTGTTCCTAGTTTTACTTGCATTGAATAATTTCCAGGATTCAGGTACA23890.1425385343291739No Hit
GGTCATATGCATTCAATCTATGCAGAGTTGATAAGGACTTGTATCCATTG23420.1397343019669005No Hit
GAGCCATACTGTATGAAAAGTTCCTCAGAGAGCAACTGTCCGAAATACAG23280.1388989987100531No Hit
CTCTTGAAGAGAGAAAAGCAATTGGAGTAAAAATGATGAAAGTACTTTTA22810.1360947663477797No Hit
CTCTTAAGGTCTGCCGCCACTGCCACTCCATGTGCTCCGTGAGATGTGTA22780.13591577279274097No Hit
TGACTACCCTGGTCAAGACCGTCTAAACAGACTAAAGAGGAAATTAGAGT22320.1331712049488138No Hit
AAATTACACTGTTGGTTCGGTGGGAAAGAATTTGACCTAGACTCTGCCTT22170.13227623717362016No Hit
CCCCTAGACCAAATGATGGAAGCATAACAGGGCCTTGCGAATCTGATGGA22050.13156026295346526No Hit
GCACAGAGTGTTCCTAGTTTTACTTGCATTGAATAATTTCCAGGATTCAG22000.13126194036173405No Hit
GCTCATAGCAGAGCAGCGAGATCCTCAGTGCCAGGAGTGAGACGGGAAAT21960.13102328228834906No Hit
GCTATGAACACAGCAAAAACAATGAATGGAATGGGAAAAGGAGAAGACGT21490.12821904992607566No Hit
CTGATATACAGAAAGCACTAATTGGTGCCTCTATCTGCTTTTTAAAACCC21320.12720475311418952No Hit
CTATTGTCCAAGCCATGAAATGGAGAGCTGATAAAATAAAAGAACAAATT21030.12547448208214848No Hit
CTCCAATGTTGCTTTGCAGTTCTTCTGCCAGTTTTTGGACGTCTTCTCCT20920.12481817238033982No Hit
GAATAAAGGGGCCAAATAAAGAGACAATAAACAGAGAGGTATCAATTTTG20870.12451984978860862No Hit
GTCTAGGGGTGTCCAAATAAGTCTTTGTGCACATCAATCTTATTTCCGCT20340.12135763031625774No Hit
GTCCAAGCCATGAAATGGAGAGCTGATAAAATAAAAGAACAAATTGAAAG20310.12117863676121901No Hit
GTCATGTACCTGAATCCTGGAAATTATTCAATGCAAGTAAAACTAGGAAC20030.11950803024752421No Hit
GTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAATGCAAGTAAAA19920.11885172054571555No Hit
ACATTACGCATATCCCTTCTTATTGTCAAACGGAACTTCCCTTCTTTCTG19800.11813574632556065No Hit
ATTCATCACAGAGCCCTTATCGGGAATGGGAACAACAGCAACAAAAAAGA19690.11747943662375197No Hit
GTATTGTCCCTGAGAGTATTAGTAAACGGAACATTCCTCAAACACCCCAA19540.11658446884855833No Hit
CTGTAAGATCATCAGTAGCAACAAGTTTAGCAACAAGCCTTCCACTCTGG19180.1144365461880936No Hit
GACCTAGACTCTGCCTTGGAATGGATAAAAAACAAAAGATGCTTAACTGA19120.11407855907801615No Hit
CTATAGAACATGCTTGTAAATTCAAACATTCCAGTTTCGTTACAGTAACT18960.11312392678447625No Hit
CAATACTCCAATGTTGCTTTGCAGTTCTTCTGCCAGTTTTTGGACGTCTT18580.11085667508731903No Hit
ATACTATACTGCTCTATTACTCAACTGCTGCTTCTAGTTTGGCTGTAACA18580.11085667508731903No Hit
GTTCAAACATTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAGC18460.11014070086716411No Hit
GGTTCAAACATTATAGGTATTTCTTCACAAGAGCTGAATTTCCCATAGAG18400.10978271375708666No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGATGACAAGC18100.10799277820669936No Hit
TAATTGTACTACTCATGGTAGTAACATCCAATGCAGATCGAATCTGCACT17720.10572552650954216No Hit
CCATAGAGGCTCTTCATTTGGGTTTTATTATCCGAATGGAACCCCCAAAC17620.10512888132607973No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCGCC151.2266533E-4145.10373145
TCCCGAA100.007076143145.10371145
ATAAGCC1100.0125.31686145
AGCACGC7850.0121.7978441
GCACGCA7950.0120.2693942
CACGCAC8850.0109.678833
ACGTGCA700.0103.478542
GGCACCC800.099.758804145
CGCACTT10450.093.584665
CGATAAA554.1472958E-1092.33874145
GAAACAC2750.092.1927264
ACGCACT10750.090.967574
GCGAGAA502.2584572E-887.062225145
GGTGCAT1000.086.924573
AAACACG3050.083.129555
CGCACCC702.910383E-1182.91641145
CGGTGCA1050.082.782842
CTTAACC451.2280179E-680.61318145
AACACGA2850.076.2609946
GGTGACC300.001930487172.551865145
Produced by FastQC (version 0.11.5)